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All Journal Biodiversitas Journal of Biological Diversity
VACHIRAPORN PIKULTHONG
Department of Science, Faculty of Science and Technology, Suan Sunandha Rajabhat University. U-Thong nok Rd., Dusit, Bangkok 10300, Thailand
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Local herbs for pain relief in the area of Tumbon Khao Hin Son, Chachoengsao, Thailand VACHIRAPORN PIKULTHONG; SUPAMAS BHIROMPAN; MANUSSAWEE DECHKLA; KANLAYA MOKKAPAN; NARUMON BOONMAN; CHANATE WANNA; KWANCHAYANAWISH MACHANA; SIRIRAT PHAKPAKNAM
Biodiversitas Journal of Biological Diversity Vol. 23 No. 10 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231007

Abstract

Abstract. Pikulthong V, Bhirompan S, Dechkla M, Mokkapan K, Boonman N, Wanna C, Machana K, Phakpaknam S. 2022. Local herbs for pain relief in the area of Tumbon Khao Hin Son, Chachoengsao, Thailand. Biodiversitas 23: 5012-5019. Local herbs have been used in Thailand for years. However, data collection on the use of local herbs in particular areas has not been thorough. This study aimed to survey local herbs treating pain relief in the area of Tumbon Khao Hin Son, Amphoe Phanom Sarakham, Chachoengsao, Thailand. Semi-structured interviews were used. Purposive sampling was used to determine the sample group. In the survey and study on botanical characteristics, from November 2019 - March 2020, 68 plants were identified and classified into 29 families. The local herbs which were mostly used for pain relief included 7 species from the Acanthaceae family, 6 types from Fabaceae, Lamiaceae, and 5 types from Zingiberaceae. The leaves of herbaceous plants had the highest frequency of use. A remedy, Ko-Klan, was found and prepared using a boiling method that could relieve pain. The surveyed herbs were used to treat 4 main symptoms which were body pain, stomachache, headache, and toothache. It was found that 36 species of herbs, or a majority of them, were used to directly relieve the pain. The interviews showed that 27% of the respondents had some home-grown herbs which were used for cooking and basic illness treatment. The age of the respondents was relevant to the use of herbs. The findings in this study will be the foundation for local herb studies which support and are aware of Thai local wisdom and knowledge as guidance to alter herbs for pharmaceutical purposes in the future.
Screening of indole-3-acetic acid PGPB from three agricultural systems at Nakhon Pathom, Thailand METHANEE HOMTHONG; WANPHEN KAEWPUK; SUKANYA YAMSUAN; ANUNYA THONGSIMA; KANLAYA MOKKAPAN; VACHIRAPORN PIKULTHONG
Biodiversitas Journal of Biological Diversity Vol. 23 No. 11 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231147

Abstract

Abstract. Homthong M, Kaewpuk W, Yamsuan S, Thongsima A, Mokkapan K, Pikulthong V. 2022. Screening of indole-3-acetic acid PGPB from three agricultural systems at Nakhon Pathom, Thailand. Biodiversitas 23: 5935-5941. Plant growth-promoting bacteria (PGPB) are associated with plant roots and enhance growth via a variety of mechanisms, including fixation of atmospheric nitrogen, solubilization of phosphorus, phytohormone synthesis, and plant phenolic compound stimulation. Sixty-five isolated bacteria from the three agricultural systems in Nakhon Pathom were screened for IAA production. The results showed that seven isolates—C01-28, C02-48, C02-51, G01-34, G03-25, G03-38, and O02-46—produced IAA under L-tryptophan as a precursor at values of 9.08 ± 0.51, 68.64 ± 29.07, 40.67 ± 18.04, 10.65 ± 1.07, 63.30 ± 1.15, 37.73 ± 1.80, and 121.28 ± 15.20 mg mL-1, respectively. Capability on plant growth-promoting traits revealed that none of the isolates stimulated ten-morning glory (Ipomoea aquatica Forsk.) seedling growth, which was not significantly different from the control. Isolates O02-46 enhanced salicylic acid (SA) in ten-morning glory seedlings the most, showing 7.18 ± 1.78 µg g-1 of fresh weight, while isolates G01-34 enhanced phenolic compound (PC) production the most showing 218.18 ± 29.55 mg GAE g-1 extract, respectively. The 16S rRNA analysis revealed that isolates O02-46, C02-48, G03-25, and C02-51 were identified as similar to Paenibacillus alvei at 99.20% (Accession number AY826588.1), Bacillus megaterium at 79.91% (Accession number MH031358.1), Bacillus sp. at 98.57% (Accession number JF322976.1), and Lysinibacillus fusiformis at 99.61% (Accession number HE610782.1).
Co-Authors ANUNYA THONGSIMA CHANATE WANNA KANLAYA MOKKAPAN KANLAYA MOKKAPAN KWANCHAYANAWISH MACHANA MANUSSAWEE DECHKLA METHANEE HOMTHONG NARUMON BOONMAN SIRIRAT PHAKPAKNAM SUKANYA YAMSUAN SUPAMAS BHIROMPAN WANPHEN KAEWPUK