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SAFIRA RAMADHANI
Profession Program in Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Kampus C Mulyorejo, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Detection of multidrug-resistant Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia GIOVANNI DWI SYAHNI PUTRA; ASWIN RAFIF KHAIRULLAH; MUSTOFA HELMI EFFENDI; MOCHAMAD LAZUARDI; SHENDY CANADYA KURNIAWAN; DANIAH ASHRI AFNANI; OTTO SAHAT MARTUA SILAEN; YUSAC KRISTANTO KHODA WARUWU; SAUMI KIREY MILLANNIA; AGUS WIDODO; SAFIRA RAMADHANI; MUHAMMAD THORIQ IHZA FARIZQI; KATTY HENDRIANA PRISCILIA RIWU
Biodiversitas Journal of Biological Diversity Vol. 24 No. 1 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240149

Abstract

Abstract. Putra GDS, Khairullah AR, Effendi MH, Lazuardi M, Kurniawan SC, Afnani DA, Silaen OSM, Waruwu YKK, Millannia SK, Widodo A, Ramadhani S, Farizqi MTI, Riwu KHP. 2023. Detection of multidrug-resistant (MDR) Staphylococcus aureus isolated from dairies milk in Medowo Village of Kediri District, Indonesia. Biodiversitas 24: 423-430. Bacterial resistance has emerged as a major concern in dairy farms in Indonesia due to the pervasive usage of antibiotics. Furthermore, no specific research has been done to explain the prevalence of Staphylococcus aureus in isolated milk from dairy cows in Kediri, particularly in Medowo Village, and their antibiotic resistance. Moreover, to control the emergence of diseases in humans brought on by dairy cow's milk or infections transmitted through milk, additional research on the prevalence and resistance of bacteria in dairy farms in the Kediri district is urgently required. In Medowo, the Village Kandangan of District, Kediri Regency, Indonesia, 100 dairy cow's milk samples were taken from farms in numerous hamlets. The Kirby-Bauer method performed an antibiotic sensitivity test using disk diffusion. The sensitivity test was attached with antibiotic discs on tetracycline, penicillin, gentamicin, erythromycin, and cefoxitin. According to sample evaluation results, S. aureus was detected in 94 (94%) of the 100 isolated milk samples based on morphological culture features, Gram staining, and biochemical assays. According to the profile of antibiotic resistance derived from the findings of the S. aureus antibiotic resistance test, 23 isolates (24.47%) were proven to be multidrug-resistant (MDR) because they were resistant to three to four classes of antibiotics. The use of antibiotics typically rises in response to an increase in the prevalence of disease in cattle, which could lead to higher levels of antibiotic residue in milk and possibly higher levels of bacterial resistance to antibiotics. Therefore, dairy farming requires methods for prudently and correctly employing antibiotics.
Phenotypic detection strategies of multidrug-resistant Staphylococcus aureus isolated from cat nasal swab in Madiun city, Indonesia SAUMI KIREY MILLANNIA; ASWIN RAFIF KHAIRULLAH; MUSTOFA HELMI EFFENDI; SUZANITA UTAMA; SHENDY CANADYA KURNIAWAN; DANIAH ASHRI AFNANI; OTTO SAHAT MARTUA SILAEN; SAFIRA RAMADHANI; SANCAKA CHASYER RAMANDINIANTO; YUSAC KRISTANTO KHODA WARUWU; AGUS WIDODO; GIOVANNI DWI SYAHNI PUTRA; MUHAMMAD THORIQ IHZA FARIZQI; KATTY HENDRIANA PRISCILIA RIWU
Biodiversitas Journal of Biological Diversity Vol. 24 No. 2 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240233

Abstract

Abstract. Millannia SK, Khairullah AR, Effendi MH, Utama S, Kurniawan SC, Afnani DA, Silaen OSM, Ramadhani S, Ramandinianto SC, Waruwu YKK, Widodo A, Putra GDS, Farizqi MTI, Riwu KHP. 2023. Phenotypic detection strategies of multidrug-resistant Staphylococcus aureus isolated from cat nasal swab in Madiun city, Indonesia. Biodiversitas 24: 940-946. Over the past ten years, the rise of bacterial disease resistance to antibiotics has presented challenges for veterinarians treating pets. Every day direct contact between pets and their owners is a risk for the spread of harmful bacteria. One of the most frequent patients in veterinary hospitals or clinics is cats. The prevalence of S. aureus infection is rising and becoming more serious as a result of antibiotic resistance, or what is known as multi-drug resistance (MDR). Pet owners and their pets may carry MDR S. aureus through zoonotic transmission. In 2018, MDR events were reported to infect ICU patients at Madiun City Hospital, East Java, Indonesia. Based on this backdrop, the authors decided to perform a study on the S. aureus bacteria resistance test of cefoxitin, tetracycline, erythromycin, gentamicin, and ampicillin bacteria obtained from cat nasal swabs in Madiun City. Making an MSA medium is the first step in bacterial isolation. Then, the Gram stain test identifies the species of Gram bacteria present in the colony. The following step is the catalase and coagulase assays, pinpointing the S. aureus bacterium. The Kirby Bauer disc diffusion method was used to conduct an antibiotic sensitivity test. With the aid of tweezers, paper discs containing the medicines cefoxitin 30 µg, tetracycline 30 µg, erythromycin 15 µg, gentamicin 10 µg and ampicillin 10 µg, are set apart from one another by 25 to 30 mm on an MHA medium. According to sample examination findings, S. aureus was present in 80 (80%), and six isolates (7.5%) were confirmed to be Multi-drug Resistance (MDR). The spread of MDR bacteria in people and animals can result in recurrent infections, a rise in complications, and an increase in morbidity and mortality. Furthermore, rational antibiotic use must be made for both humans and animals to stop the emergence of antibiotic resistance in the veterinary clinic in Madiun City.
Co-Authors AGUS WIDODO AGUS WIDODO ASWIN RAFIF KHAIRULLAH ASWIN RAFIF KHAIRULLAH DANIAH ASHRI AFNANI GIOVANNI DWI SYAHNI PUTRA KATTY HENDRIANA PRISCILIA RIWU KATTY HENDRIANA PRISCILIA RIWU MOCHAMAD LAZUARDI MUHAMMAD THORIQ IHZA FARIZQI MUSTOFA HELMI EFFENDI OTTO SAHAT MARTUA SILAEN OTTO SAHAT MARTUA SILAEN SANCAKA CHASYER RAMANDINIANTO SAUMI KIREY MILLANNIA SHENDY CANADYA KURNIAWAN SHENDY CANADYA KURNIAWAN SUZANITA UTAMA YUSAC KRISTANTO KHODA WARUWU