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AMALIA SUTRIANA
Laboratory of Pharmacology, Faculty of Veterinary Medicine, Universitas Syiah Kuala. Jl. Teungku Hasan Krueng Kalee No. 4, Banda Aceh 23111, Aceh, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Phytocomponents analysis and antioxidant activity of Malacca fruit extract (Phyllanthus emblica) using three different solvents NAZARUDDIN NAZARUDDIN; ROSMAIDAR ROSMAIDAR; TONGKU N. SIREGAR; SRI WAHYUNI; AMALIA SUTRIANA
Biodiversitas Journal of Biological Diversity Vol. 25 No. 5 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250507

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Abstract. Nazaruddin, Rosmaidar, Siregar TN, Wahyuni S, Sutriana A. 2024. Phytocomponents analysis and antioxidant activity of Malacca fruit extract (Phyllanthus emblica) using three different solvents. Biodiversitas 25: 1911-1919. Malacca plant (Phyllanthus emblica L.) grows widely in Aceh Province, Indonesia representing a wide group of phytochemical components of medicinal usage. This study aims to assess the antioxidant activity and bioactive compounds in P. emblica fruit extract, which was extracted by subsequent extraction using n-hexane, ethyl acetate, and ethanol. The extracts were then subjected to phytochemical and GC-MS to identify and quantify the bioactive compounds and measure the radical scavenging using DPPH assay to estimate the antioxidant activity. The results showed that P. emblica fruit extracted with ethanol and ethyl acetate contained flavonoids, phenolics, terpenoids, and saponin compounds, while n-hexane extract only contained steroids. The antioxidant activity of ethanol, ethyl acetate, and n-hexane extracts was very strong, with IC50 values of 18.81, 20.75, and 31.01 ppm, respectively. The highest antioxidant activity was found in the ethanol extract of P. emblica fruit. The GC-MS analysis revealed that the most abundant chemical component in ethanol and ethyl acetate extract was 1, 2, 3 benzenetriol, while in n-hexane extract was eugenol. Based on bioactive components and antioxidant activity, ethanol is suggested as the optimal solvent to gain a high content of polyphenols and flavonoids as well as high antioxidants from P. emblica fruit for utilization in pharmacognosy.
Moringa oleifera as a potential bioactive agent against Gram-positive and negative bacteria: In-silico analysis of 1YN5 and 3RG1 receptor binding CUT NILA THASMI; HAFIZUDDIN HAFIZUDDIN; HUSNURRIZAL HUSNURRIZAL; DASRUL DASRUL; AMALIA SUTRIANA; BASRI A. GANI; MUHAMMAD NAZAR
Biodiversitas Journal of Biological Diversity Vol. 25 No. 8 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250812

Abstract

Abstract. Thasmi CN, Hafizuddin H, Husnurrizal H, Dasrul, Sutriana A, Gani BA, Nazar M. 2024. Moringa oleifera as a potential bioactive agent against Gram-positive and negative bacteria: In-silico analysis of 1YN5 and 3RG1 receptor binding. Biodiversitas 25: 3411-3421. The natural antioxidant extract of Moringa oleifera Lam. simultaneously possesses anti-inflammatory, anti-infertility, antimicrobial, and antioxidant properties. This research aims to investigate the active chemical compounds isolated from the leaves of M. oleifera and examine the chemical interaction between the selected compounds and the 1YN5 and 3RG1 proteins based on the molecular docking model. M. oleifera leaves were collected from the Kajhu Village, District of Aceh Besar, Province of Aceh, Indonesia. The bioactive compounds were isolated through cold extraction by using the maceration technique. Chemical analysis and characterization of bioactive compounds were carried out by Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography-Mass Spectrometry (GC-MS). The antioxidant bioactivity/Free Radical Scavenging Potency (FRSP) was determined using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical assay. The molecular docking process refers to the modified MOE software docking protocol. The data obtained were analyzed descriptively. The ethanolic extract of M. oleifera leaf contains 38 bioactive compounds. Several bioactive compounds have high peak area percentages, including Oxirane, hexadecyl- (20.22%), TRICYCLO [20.8.0.0E7,16] TRIACONTA (14.35%), acetic acid (CAS), ethyl acetate (11.32%), n-Hexadecanoic acid (8.94%), and oleic acid (8.12%). Molecular docking showed that oleic acid, oxirane, hexadecyl, and 1-Heptacosanol have greater affinity to the 1YN5 gene, while octadecanoic acid and tetracontane have better binding affinity to the 3RG1 proteins. The bioactive compound 1-Heptacosanol bound both to the 1YN5 and 3RG1 proteins. The ethanolic extract of M. oleifera leaves possessed better IC50 (6.07 ppm), relatively twice lower when compared to the positive standard, Vitamin C (10.58 ppm). The M. oleifera ethanol extract exhibits antioxidant bioactivity in the very strong category. The bioactive compound 1-Heptacosanol shows binding affinity with both 1YN5 and 3RG1 proteins.
Co-Authors BASRI A. GANI CUT NILA THASMI DASRUL DASRUL HAFIZUDDIN HAFIZUDDIN HUSNURRIZAL HUSNURRIZAL MUHAMMAD NAZAR NAZARUDDIN NAZARUDDIN ROSMAIDAR ROSMAIDAR SRI WAHYUNI TONGKU N. SIREGAR