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All Journal Biodiversitas Journal of Biological Diversity
ILHAM ZULFAHMI
Department of Fisheries Resources Utilization, Faculty of Marine and Fisheries, Universitas Syiah Kuala. Jl. Teuku Nyak Arief Darussalam, Banda Aceh 24415, Aceh, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Brine shrimp cytotoxicity bioassay of red mangrove (Rhizophora mucronata) leaves using different solvents and its potency as antibacterial ILHAM ZULFAHMI; EPA PAUJIAH; ZULTIRA HARINA ROZA; KAMALIAH HELMI; BADRATUN NAFIS; FIRMAN MUHAMMAD NUR; PUTROE NURUL FAZILA
Biodiversitas Journal of Biological Diversity Vol. 25 No. 9 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250945

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Abstract. Zulfahmi I, Paujiah E, Roza ZH, Helmi K, Nafis B, Nur FM, Fazila PN. 2024. Brine shrimp cytotoxicity bioassay of red mangrove (Rhizophora mucronata) leaves using different solvents and its potency as antibacterial. Biodiversitas 25: 3227-3236. This study evaluates the cytotoxicity of ethanol, ethyl acetate, and n-hexane extracts of red mangrove (Rhizopora mucronata) leaves and their potential against pathogenic bacteria. Red mangrove leaf was evaluated for its cytotoxicity using the Brine Shrimp Lethality Test (BSLT). Each solvent's LC50 - 24-hour value was determined using probit analysis and measured using one-way ANOVA with a 95% confidence interval (P<0.05). The secondary metabolic compounds in each extract were presented and analyzed descriptively using GC-MS analysis. The effect of incubation time on the bacterial inhibition zone was tested using independent t-test analysis against S. aureus, V. parahaemolyticus, and A. hydrophyla. The results showed that the LC50 value of ethanol extract was 402.45 ppm, ethyl acetate extract was 230.97 ppm, and n-hexane extract was 180.31 ppm. The secondary metabolite compounds observed in red mangrove leaves extracted with different solvents (ethanol, ethyl acetate, and n-hexane) include terpenoids, alkaloids, ketones, phenols, turpentine oil, essential oils, unsaturated fats, and camphor. The ethyl acetate extract from red mangrove leaves inhibited the growth of S.aureus and V. parahaemolyticus. A. hydrophila and V. parahaemolyticus exhibited the lowest inhibition zone, which varied based on time and concentration.
Morphological diversity of fish size and otolith dimension of the protandrous hermaphroditic fish Eleutheronema tetradactylum from Pattani Bay, Thailand TEUKU HARIS IQBAL; SUKREE HAJISAMAE; SOFIYUDIN MAAE; ILHAM ZULFAHMI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 4 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260430

Abstract

Abstract. Iqbal TH, Hajisamae S, Maae S, Zulfahmi I. 2025. Morphological diversity of fish size and otolith dimension of the protandrous hermaphroditic fish Eleutheronema tetradactylum from Pattani Bay, Thailand. Biodiversitas 26: 1807-1815. This study examines the morphological diversity of otolith dimensions across different sexes and size classes of Eleutheronema tetradactylum from Pattani Bay, Thailand. A total of 303 specimens (166 males, 137 females) were collected using monofilament gillnets from May to December 2021. Measurements of Standard Length (SL) and Body Weight (BW), Otolith Length (OL), Otolith Height (OH), and Otolith Weight (OW) were taken to the nearest size of 0.01 mm and weight of 0.001 g. Ordinary Least Squares (OLS) regression, Student's t-test, and One-way Analysis of Variance (ANOVA) were applied to estimate relationships and significant differences between sexes and size classes. The highest correlation was observed between SL and BW with OL in males (R2: 0.95). Significant differences were found in otolith parameters between sexes and size classes; females exhibited significantly larger otoliths than males, and the larger size of fish was also associated with significantly larger otoliths than those of smaller fish (P<0.01). These findings highlight the correlation between fish size and otolith dimensions, demonstrating otolith analysis as a useful tool for growth estimation and stock differentiation of E. tetradactylum populations.
Co-Authors BADRATUN NAFIS EPA PAUJIAH FIRMAN MUHAMMAD NUR KAMALIAH HELMI PUTROE NURUL FAZILA SOFIYUDIN MAAE SUKREE HAJISAMAE TEUKU HARIS IQBAL ZULTIRA HARINA ROZA