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All Journal Biodiversitas Journal of Biological Diversity
ANKARDIANSYAH PANDU PRADANA
Department of Plant Protection, Faculty of Agriculture, Universitas Jember. Jl. Kalimantan No. 37, Jember 68121, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Molecular detection of Dorylaimus stagnalis (Durjan, 1845), in the rhizosphere of soybean plants in Jember District, Indonesia ANKARDIANSYAH PANDU PRADANA; ZIDNA NURUL IZZATIKA; HARDIAN SUSILO ADDY
Biodiversitas Journal of Biological Diversity Vol. 26 No. 4 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260440

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Abstract. Pradana AP, Izzatika ZN, Addy HS. 2025. Molecular detection of Dorylaimus stagnalis (Durjan, 1845), in the rhizosphere of soybean plants in Jember District, Indonesia. Biodiversitas 26: 1913-1919. Nematodes, members of the phylum Nematoda, are ubiquitous soil organisms whose community composition provides critical insights into soil health and ecosystem function. The genus Dorylaimus is of particular ecological interest due to its adaptability and role in nutrient cycling within diverse terrestrial environments. This study documents the first molecular and morphometric confirmation of Dorylaimus stagnalis (Dujardin, 1845), in the rhizosphere of soybean (Glycine max) in Jember District, Indonesia. Soil samples were systematically collected from 15 distinct sites within soybean fields using a standardized protocol, and nematodes were extracted via the Whitehead tray method. Detailed morphological observations were made using an Olympus BX50 light microscope, and morphometric measurements were obtained following De Man’s ratios, which collectively confirmed diagnostic features consistent with D. stagnalis as reported in previous studies. Molecular characterization was performed by PCR amplification of the 28S-D2D3 region followed by Sanger sequencing. Subsequent phylogenetic analysis revealed 94% sequence similarity to established reference sequences (AY592994.1 and AY592995.1) and a low genetic distance of 0.1058, thereby substantiating the taxonomic identity of the specimens. This study’s integrative approach not only verifies the presence of D. stagnalis in Indonesia but also indicates subtle morphometric variations potentially attributable to local environmental pressures. Its findings expand the current understanding of nematode biodiversity in Indonesian agroecosystems and provide a framework for future research on sustainable soil management practices.
Iron(II) chloride enhances the biocontrol activity of Pseudomonas fluorescens against root-knot nematodes ANKARDIANSYAH PANDU PRADANA; MOHAMMAD HOESAIN; IIS NUR ASYIAH; MUH ADIWENA; YELVI ANGELINA; DIANA PUTRI; RACHMI MASNILAH; RIFANI RUSIANA DEWI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 8 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260850

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Abstract. Pradana AP, Hoesain M, Asyiah IN, Adiwena M, Angelina Y, Putri D, Masnilah R, Dewi RR. 2025. Iron(II) chloride enhances the biocontrol activity of Pseudomonas fluorescens against root-knot nematodes. Biodiversitas 26: 4243-4256. Root-knot nematodes (Meloidogyne incognita) account for over 15% of global crop losses, highlighting the urgent need for sustainable biocontrol solutions. Microbial-based strategies, particularly those employing Pseudomonas fluorescens, present environmentally responsible alternatives to chemical nematicides while enhancing soil microbial diversity. The aim of this study was to evuluate the systematic assessment of Iron(II) Chloride (FeCl?) supplementation for improving the nematicidal efficacy of P. fluorescens. Five treatment combinations of FeCl? (0, 50, and 75 ppm) and pH (6 and 8) were evaluated in vitro. The results showed that at 24 hours, J2 mortality reached 16.8% in the 75 ppm FeCl? treatments (pH 6 and 8), significantly exceeding the control (4.4%, p<0.05). By 96 hours, FeCl?-fortified cultures achieved 55.6-56.4% J2 mortality, compared to 35.0% in the control (p<0.05). At 168 hours, maximum J2 mortality of 97.0% was recorded with 75 ppm FeCl? at pH 8, nearly three times higher than the control (p<0.01). Similarly, inhibition of egg hatching was markedly enhanced; after 168 hours, 82.8% of eggs remained unhatched in the 50 ppm FeCl? at pH 6 treatment, versus 29.8% in the control (p<0.01). Gas Chromatography-Mass Spectrometry analysis revealed a significant increase in bioactive fatty acid derivatives, such as methyl linoleate, in FeCl?-supplemented cultures, supporting a mechanism of nematode membrane disruption. Collectively, these findings demonstrated that FeCl? supplementation significantly enhances the nematicidal activity of P. fluorescens, offering a novel and effective strategy for sustainable management of root-knot nematodes. This approach offers considerable potential for integration into environmentally compatible crop protection strategies, contributing to the development of sustainable nematode management practices in agricultural systems.
Species composition and abundance of weeds in tomato fields with nematicidal potential against Meloidogyne incognita MUH. ADIWENA; ADITYA MURTILAKSONO; ANKARDIANSYAH PANDU PRADANA; AISYAH RIZKY PARAMESWARI; DIANA PUTRI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 11 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261145

Abstract

Abstract. Adiwena M, Murtilaksono A, Pradana AP, Parameswari AR, Putri D. 2025. Species composition and abundance of weeds in tomato fields with nematicidal potential against Meloidogyne incognita. Biodiversitas 26: 5891-5905. Root-knot nematodes (Meloidogyne incognita) cause major crop losses worldwide, yet sustainable management options remain limited. This study assessed weed community structure in tomato fields and tested the nematicidal activity of dominant species. Quadrat sampling (1×1 m) recorded seven species from six families, with moderate diversity (Shannon-Wiener H?: 1.125), low evenness (E: 0.578), and high dominance (Simpson’s D: 0.470). Alternanthera philoxeroides was ecologically dominant (IVI: 72.0%; RD: 62.5%), while Eleusine indica and Cyperus rotundus were most frequent (RF: 23.8%). Aqueous root extracts (20% w/v) were tested in vitro, and proportion data were arcsine square-roots transformed and analyzed using one-way ANOVA followed by Tukey’s HSD test (?: 0.05). A. philoxeroides achieved the strongest egg-hatch inhibition (98.2±0.45% unhatched eggs at 168 h vs. 69.6±3.58% in control), while Cleome rutidosperma induced the highest J2 mortality (21.8±6.14%). Gas chromatography-mass spectrometry (GC-MS) revealed 12 constituents in A. philoxeroides and 18 in C. rutidosperma. Key compounds included methyl palmitate, methyl oleate, and methyl linoleate in A. philoxeroides; and lauric acid, methyl palmitate, and 9,12-octadecadien-1-ol in C. rutidosperma. These phytochemicals are consistent with the observed suppression of egg hatching and juvenile survival. To our knowledge, this is the first study in Indonesia to explicitly link weed community diversity with in vitro nematicidal activity against M. incognita in tomato fields. By integrating ecological survey and bioassay data, this work demonstrates that naturally occurring weeds can serve both as indicators of agroecosystem health and as practical, locally available sources of botanical nematicides. The findings emphasize two major contributions: first, to nematode management through eco-friendly suppression of root-knot nematodes; and second, to biodiversity conservation by recognizing weeds as functional components of sustainable agroecosystems.
Co-Authors ADITYA MURTILAKSONO AISYAH RIZKY PARAMESWARI HARDIAN SUSILO ADDY IIS NUR ASYIAH MOHAMMAD HOESAIN MUH ADIWENA MUH. ADIWENA Putri Diana RACHMI MASNILAH RIFANI RUSIANA DEWI YELVI ANGELINA ZIDNA NURUL IZZATIKA