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All Journal Jurnal Fitopatologi Indonesia Innofarm Jurnal Inovasi Pertanian Jurnal Sumberdaya Hayati Jurnal Pertanian Terpadu Caraka Tani: Journal of Sustainable Agriculture Jurnal Kridatama Sains dan Teknologi ZIRAA'AH MAJALAH ILMIAH PERTANIAN Agrica: Journal of Sustainable Dryland Agriculture Jurnal HPT (Hama Penyakit Tumbuhan) Biodiversitas Journal of Biological Diversity
Putri Diana
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Andalas
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences

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Eksplorasi Bakteri Endofit dari Akar Tanaman Adam Hawa dan Potensinya sebagai Agens Hayati dan Pemacu Pertumbuhan Tanaman Padi Ankardiansyah Pandu Pradana; Diana Putri; Abdul Munif
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.157 KB) | DOI: 10.14692/jfi.11.3.73

Abstract

Rhoeo discolor has been known to have a good adaptation to various environmental conditions. This character might be due to mutualistic association with endophytic bacteria. The objective of this study was to isolate endophytic bacteria from roots of R. discolor and to evaluate their potency as biocontrol agents and plant growth promoters. The methods to isolate endophytic bacteria involved the following methods, sterilization of root surface, grinding of root tissues, dilution, and plating in the medium tryptone soya agar (TSA). A total of 21 isolates of endophytic bacteria were isolated from the roots of R. discolor. Based on hypersensitivity test on tobacco leaves, 19 isolates showed negative reaction (no necrosis symptom) and only 2 isolates showed positive reaction (necrosis was developed). The results on biocontrol and growth promoters assay showed that 7 isolates were able to inhibit the growth of Fusarium oxysporum under in vitro test and 12 isolates were able to increase the growth of rice seedlings.
Potensi Seduhan Limbah Baglog Jamur Tiram (Pleurotus ostreatus) dalam Menekan Populasi Radopholus similis dan Meloidogyne spp. pada Tanah Asal Perakaran Tanaman Lada (Piper nigrum L) ANKARDIANSYAH PANDU PRADANA; DIANA PUTRI; ABDUL MUNIF
Jurnal Sumberdaya Hayati Vol. 2 No. 2 (2016)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.2.2.39-44

Abstract

Indonesia is one of the largest pepper producing countries in the world. One of the pepper-producing provinces in Indonesia is the Bangka Belitung Islands (Babel). However, the infection of Radopholus similis and Meloidogyne spp. which causes yellow disease becomes one of obstacles in pepper cultivation in Babel. Thus, alternative solution to overcome this yellow disease is by reducing the amount of nematode inoculum of R. similis and Meloidogyne spp. in soil. This study aimed to determine the effectiveness of the infusion of oyster mushrooms baglog waste enriched with molasses to control the population of R. similis and Meloidogyne spp. and to increase the population of rhizobacteria. Infusion of oyster mushrooms baglog waste was mixed with 1% of molasses and was poured into soil obtained from the pepper plant roots. The concentrations used were 10%, 20%, 30% and 50%. Population of phytonematode, rhizobacteria, proteolytic bacteria, and group of fluorescence bacteria were calculated before and 7 days after treatment. Results showed that the populations of R. similis and Meloidogyne spp. in the soil poured with the influsion of oyster mushroom baglog waste decreased by 29.11% (R. similis) and 24.61% (Meloidogyne spp.), compared to the before and control treatments. The suppression of nematode population was found to be the highest in the influsion treatment at concentration of 50%. Overall, treatment of all concentrations succeeded to increase the population of rhizobacteria, proteolytic bacteria, and group of fluorescence bacteria in soil. Moreover, the highest increase was found in soil treated with concentration of 50%. This study provided new information that the infusion application of oyster mushrooms baglog waste enriched with molasses had the potential to increase the population of rhizobacteria and suppress the amount of pathogens R. similis and Meloidogyne spp. 
Pengaruh Beberapa Dosis Abu Janjang Kelapa Sawit Terhadap Pertumbuhan Bibit Kakao (Theobroma cacao L.) Siska Efendi; Putri Diana; Nasrez Akhir
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 45, No 1 (2020)
Publisher : Pusat Publikasi Jurnal Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v45i1.2601

Abstract

          The plant medium condition in the nursery will affect the growth of cacao seedlings. The oil Palm ash is one of the soil ameliorants because it content complete nutrient both macro and micro, the capability to increasing soil pH and having a high base saturation. The objectives of the research were to study the effect of oil palm ash on the growth of cacao seedlings and to get the dose for cacao in nurseries. This research was conducted in experimenta field of 3rd Campus Andalas University Dharmasraya, in December 2018 until March 2019. The experimental method used was a Completely Randomized Design (CRD) consisted of 5 treatments and 5 replications, each of experiment unit consistent of 2 plant polybag so the total of 50 plants was obtained. The Treatments were some doses of oil palm ash of 0 g of oil palm ash/polybag, 150 g of oil palm ash/polybag, 300 g of oil palm ash/polybag, 450 g of palm oil ash/polybag, and 600 g of oil palm ash/polybag. Data analysis was performed by analysis of variance (F test) at the level of 5%. If its results are significant, then further tests with DNMRT at the level of 5%. The results of the present study showed that oil palm ash had an influence on the growth of cacao seedlings on the variables of stem height, stem diameter, number of leaves, and the root length. The dosage of 450 g oil palm ash/polybag showed the best results for stem height and number of leaves of cacao seedlings, while the dosage of 600 g oil palm ash/polybag showed the best results for stem diameter and root length of cacao seedlings. The recommended dose for cacao in the nursery is 450 g of oil palm ash/polybag. 
Interaksi Faktor Lingkungan dan Sistem Budidaya terhadap Serangan Penyakit Karat Daun pada Tanaman Jagung Putri, Diana
Jurnal Kridatama Sains dan Teknologi Vol 7 No 01 (2025): Jurnal Kridatama Sains dan Teknologi
Publisher : Universitas Ma'arif Nahdlatul Ulama Kebumen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53863/kst.v7i01.1636

Abstract

Corn is an important food crop in the world besides rice and wheat. In Indonesia, corn is not only used as food but also as animal feed and industrial materials, so the need for corn will continue to increase. Many obstacles are limiting factors in meeting the needs and productivity of corn, one of which is the attack of diseases such as corn leaf rust disease by Puccinia sp. This study aims to determine the incidence and severity of leaf rust disease attacks on monoculture and polyculture lands and to determine the importance of environmental factors on the development of the disease. The study was conducted in the experimental field and Phytopathology Laboratory of the Agroecotechnology Study Program, Campus III, Andalas University, Dharmasraya. The study was descriptive in nature using a survey method. Observation parameters included land conditions, symptoms of corn leaf rust, microscopic identification of pathogens, and the incidence and severity of the disease. The results showed that the pathogen causing leaf rust was identified as Puccinia polysora with symptoms of attack in the form of brownish yellow spots (pustules). The high incidence of the disease reached 100% while the severity reached 11.42% in polyculture lands and 15.00% in monoculture lands. This shows that the severity of corn leaf rust disease in the experimental land of the Agroecotechnology Study Program is included in the mild category
INSIDENSI DAN SEVERITAS PENYAKIT PADA PERKEBUNAN GAMBIR (Uncaria gambir Roxb) DESA DURIAN TINGGI KABUPATEN LIMA PULUH KOTA, SUMATERA BARAT: INCIDENCE AND SEVERITY OF PLANT DISEASE OF GAMBIER (Uncaria gambir Roxb) CROPS DURIAN TINGGI VILLAGE, LIMA PULUH KOTA REGENCY, WEST SUMATERA Waruwu, Arifda Ayu Swastini; Putri, Diana
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 13 No. 3 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2025.013.3.4

Abstract

Kabupaten Lima Puluh Kota merupakan salah satu daerah penghasil gambir utama di Sumatera Barat. Berbagai tanaman, termasuk Uncaria gambir, rentan terserang penyakit. Penelitian ini bertujuan untuk menganalisis insidensi dan severitas penyakit yang menyerang U. gambir. Penelitian dilakukan pada bulan Mei 2025 dengan menggunakan metode Purposive Random Sampling (PRS) dan survei lapangan di tiga lokasi di Nagari Durian Tinggi. Wawancara dengan petani yang bekerja di perkebunan gambir juga dilakukan untuk melengkapi data hasil pengamatan mengenai gejala penyakit. Berbagai jenis penyakit bercak daun berhasil didokumentasikan, antara lain bercak ungu, bercak hitam, dan bercak coklat. Dari ketiga jenis tersebut, bercak ungu merupakan penyakit yang paling dominan. Insidensi penyakit tertinggi mencapai 100% di semua lahan. Tingkat severitas bercak ungu tertinggi terjadi di Lahan 1 sebesar 74%, sedangkan severitas terendah ditemukan di Lahan 2 dan 3 sebesar 51% .
INSIDENSI DAN INTENSITAS BEBERAPA PENYAKIT UTAMA TANAMAN GAMBIR DI KABUPATEN LIMA PULUH KOTA Putri, Diana; Waruwu, Arifda Ayu Swastini; Pradana, Ankardiansyah Pandu
Innofarm : Jurnal Inovasi Petanian Vol 27 No 2 (2025): JURNAL INNOFARM : Jurnal Inovasi Petanian
Publisher : FAKULTAS PERTANIAN UNIVERSITAS SLAMET RIYADI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33061/innofarm.v27i2.12785

Abstract

Gambir is one of Indonesia’s superior commodities in the international market. However, problems that causes a decrease in the quality and productivity of gambir plants is disease attacks. The percentage and intensity of disease attacks on gambir plants in Lima Puluh Kota Regency have never been reported. This studi aimed termine the level of attack occurrence and intensity of diseases that attack gambir plants as basic information related to plant health status in an effort to manage diseases effectively. The study was conducted in three gambir land locations, using a survey method with Simple Random Sampling. Direct observation includes symptoms of attack, percentage and intensity of disease. The results of observations on land 1 and 2 found five main diseases namely red rust, brown spots, black spots, black mosaic and chlorosis, while on land 3 brown spot disease, dieback and black mosaic were found. The highest incidence of disease on land 1 was red rust which reached 100%, on land 2 red rust and brown spots were 100%, and on land 3 brown spot disease was 96%. The highest intensity of disease attack was red rust reaching 82% which was included in the category of very severe attacks. The high incidence and intensity of attacks of this disease are influenced by the age of the plants and the cultivation techniques used by gambir farmers, such as the lack of weed and disease control and fertilization. Keywords: Cultivation, disease intensity, gambir, red rust
Eksplorasi dan Karakterisasi Bakteri Endofit Pada Tanaman Gambir (Uncaria gambir Roxb.) di Kabupaten Lima Puluh Kota Diana Putri; Ankardiansyah Pandu Pradana; Arifda Ayu Swastini Waruwu
Jurnal Pertanian Terpadu Vol 14 No 1 (2026): Jurnal Pertanian Terpadu Jilid XIV Nomor 1 Juni 2026
Publisher : Sekolah Tinggi Pertanian Kutai Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36084/jpt..v14i1.741

Abstract

Bakteri endofit dapat ditemukan dari semua jaringan tanaman tetapi tidak menyebabkan gangguan atau kerusakan pada tanaman tersebut. Tujuan penelitian untuk mengeksplorasi dan mengkarakterisasi morfologi bakteri endofit dari akar tanaman gambir. Sampel akar gambir diambil dari Sumatera Barat, Kabupaten Lima Puluh Kota dari 5 titik Lokasi yang berbeda. Isolasi dilakukan untuk memperoleh isolat tunggal bakteri endofit. Karakterisasi meliputi pengamatan morfologi koloni, uji gram dengan KOH 3% serta uji patogenesitas pada daun tembakau. Hasil isolasi diperoleh 23 isolat tunggal bakteri endofit, dimana dari pengamatan morfologi didominasi oleh bentuk koloni yang tidak beraturan, permukaan yang halus tidak mengkilap, elevasi datar dan warna koloni putih susu. Dari 23 isolat yang diuji gram dengan KOH 3%, sebanyak 11 isolat tergolong gram negatif dan 12 isolat lainnya kelompok gram positif. Uji patogenesitas pada daun tembakau diperoleh hasil sebanyak 7 isolat menunjukkan reaksi hipersensitif yang ditandai dengan gejala nekrosis, sedangkan 16 isolat lainnya tidak menunjukkan gejala nekrosisi pada daun tembakau
EKSPLORASI DAN KARAKTERISASI BAKTERI ENDOFIT DARI TANAMAN KOPI SOLOK RADJO, SUMATERA BARAT Diana Putri; Arifda Ayu Swastini Waruwu; Sri Heriza; Zahlul Ikhsan
AGRICA Vol. 19 No. 1 (2026): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v19i1.6901

Abstract

Endophytic bacteria in a plant tissue do not cause disturbance or damage to the plant. Phytohormones produced by endophytic bacteria can increase plant growth and function as antimicrobials. The study aims to explore, select, and characterize the morphology of endophytic bacteria originating from the stems and roots of coffee plants from Solok Radjo. Morphological observations were carried out after obtaining a single isolate of endophytic bacteria, then a Gram test with 3% KOH and a hypersensitive reaction test on water henna plants were carried out to determine the pathogenicity of the endophytic bacteria. A total of 35 isolates were obtained from the isolation results, namely 23 isolates from the stem and 12 isolates from the roots. The results of morphological observations of endophytic bacterial colonies on Nutrient Agar (NA) media were dominated by irregular colony shapes, smooth and shiny surfaces, flat elevations, and yellowish-white colony colors. Gram testing with 3% KOH obtained 17 isolates classified as Gram-positive and 18 isolates as Gram-negative. Hypersensitive observations that showed symptoms of necrosis (positive reaction) were 19 isolates, and no symptoms of necrosis (negative reaction) were 16 isolates.
Antifungal Activity of Rice Husk-Derived Liquid Smoke: Growth Suppression of Rhizoctonia solani and Bioactive Compound Profiling Ankardiansyah Pandu Pradana; Deril Indana Damayanti; Muh Adiwena; Ahmed Ibrahim Alrashid Yousif; Diana Putri
Caraka Tani: Journal of Sustainable Agriculture Vol 40, No 3 (2025): July
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v40i3.95289

Abstract

Sheath blight poses a major challenge in rice cultivation, with current control methods relying heavily on synthetic fungicides. Environmentally sustainable alternatives, such as bioactive compounds derived from agricultural waste, offer promising potential for integrated disease management. This study investigated the antifungal efficacy of rice husk-derived liquid smoke against Rhizoctonia solani, the causal agent of rice sheath blight, and identified its active compounds using gas chromatography-mass spectrometry (GC-MS) analysis. Liquid smoke was produced through the pyrolysis of rice husks and incorporated into potato dextrose agar at concentrations of 1%, 2%, 3%, 4%, and 5% (v/v). R. solani was isolated from infected rice plants, and its pathogenicity was confirmed on rice seeds and seedlings. The in vitro antifungal activity was assessed by measuring colony diameter and calculating the percentage of mycelial growth inhibition over 7 days. The results demonstrated that liquid smoke significantly inhibited the growth of R. solani in a concentration-dependent manner (p < 0.001; exact p = 4.36 × 10-²⁴), with the 5% concentration achieving 100% inhibition. Qualitative microscopic observations revealed morphological abnormalities in fungal hyphae at higher concentrations. GC-MS analysis identified 40 bioactive compounds in the liquid smoke, including phenolic compounds and organic acids, which are known for their antimicrobial properties. The findings suggest that rice husk-derived liquid smoke possesses potent antifungal activity against R. solani due to the presence of these compounds. This study concludes that rice husk liquid smoke can serve as an effective, eco-friendly alternative to synthetic fungicides for controlling sheath blight disease in rice cultivation.
Iron(II) chloride enhances the biocontrol activity of Pseudomonas fluorescens against root-knot nematodes ANKARDIANSYAH PANDU PRADANA; MOHAMMAD HOESAIN; IIS NUR ASYIAH; MUH ADIWENA; YELVI ANGELINA; DIANA PUTRI; RACHMI MASNILAH; RIFANI RUSIANA DEWI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 8 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260850

Abstract

Abstract. Pradana AP, Hoesain M, Asyiah IN, Adiwena M, Angelina Y, Putri D, Masnilah R, Dewi RR. 2025. Iron(II) chloride enhances the biocontrol activity of Pseudomonas fluorescens against root-knot nematodes. Biodiversitas 26: 4243-4256. Root-knot nematodes (Meloidogyne incognita) account for over 15% of global crop losses, highlighting the urgent need for sustainable biocontrol solutions. Microbial-based strategies, particularly those employing Pseudomonas fluorescens, present environmentally responsible alternatives to chemical nematicides while enhancing soil microbial diversity. The aim of this study was to evuluate the systematic assessment of Iron(II) Chloride (FeCl?) supplementation for improving the nematicidal efficacy of P. fluorescens. Five treatment combinations of FeCl? (0, 50, and 75 ppm) and pH (6 and 8) were evaluated in vitro. The results showed that at 24 hours, J2 mortality reached 16.8% in the 75 ppm FeCl? treatments (pH 6 and 8), significantly exceeding the control (4.4%, p<0.05). By 96 hours, FeCl?-fortified cultures achieved 55.6-56.4% J2 mortality, compared to 35.0% in the control (p<0.05). At 168 hours, maximum J2 mortality of 97.0% was recorded with 75 ppm FeCl? at pH 8, nearly three times higher than the control (p<0.01). Similarly, inhibition of egg hatching was markedly enhanced; after 168 hours, 82.8% of eggs remained unhatched in the 50 ppm FeCl? at pH 6 treatment, versus 29.8% in the control (p<0.01). Gas Chromatography-Mass Spectrometry analysis revealed a significant increase in bioactive fatty acid derivatives, such as methyl linoleate, in FeCl?-supplemented cultures, supporting a mechanism of nematode membrane disruption. Collectively, these findings demonstrated that FeCl? supplementation significantly enhances the nematicidal activity of P. fluorescens, offering a novel and effective strategy for sustainable management of root-knot nematodes. This approach offers considerable potential for integration into environmentally compatible crop protection strategies, contributing to the development of sustainable nematode management practices in agricultural systems.
Co-Authors Abdul Munif ABDUL MUNIF ADITYA MURTILAKSONO Ahmed Ibrahim Alrashid Yousif AISYAH RIZKY PARAMESWARI ANKARDIANSYAH PANDU PRADANA Ankardiansyah Pandu Pradana Ankardiansyah Pandu Pradana Ankardiansyah Pandu Pradana Ankardiansyah Pandu Pradana Arifda Ayu Swastini Waruwu Deril Indana Damayanti IIS NUR ASYIAH MOHAMMAD HOESAIN MUH ADIWENA Muh Adiwena MUH. ADIWENA Nasrez Akhir RACHMI MASNILAH RIFANI RUSIANA DEWI Siska Efendi Sri Heriza Waruwu, Arifda Ayu Swastini YELVI ANGELINA Zahlul Ikhsan