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All Journal Biodiversitas Journal of Biological Diversity
IDA AYU PUTRI DARMAWATI
Department of Agroecotechnology, Faculty of Agriculture, Universitas Udayana. Jl. PB. Sudirman, Denpasar 80231, Bali, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Genetic diversity and mutant detection of Dendrobium bicaudatum using SRAP markers IDA AYU PUTRI DARMAWATI; YUYUN FITRIANI; GEDE WIJANA; WAFA’ NUR HANIFAH; RINDANG DWIYANI; NI LUH MADE PRADNYAWATHI; FITRIA DWI DARMAYATI
Biodiversitas Journal of Biological Diversity Vol. 27 No. 2 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270223

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Abstract. Darmawati IAP, Fitriani Y, Wijana G, Hanifah WN, Dwiyani R, Pradnyawathi NLM, Darmayati FD. 2026. Genetic diversity and mutant detection of Dendrobium bicaudatum using SRAP markers. Biodiversitas 27 (2): d270223. https://doi.org/10.13057/biodiv/d270223. Natural occurring mutations have been an important source of genetic diversity and new phenotypes for ornamental plants. However, the occurrence rate is relatively low for most plants, including Dendrobium bicaudatum. Mutation induction triggers mutations in plants and can be achieved through physical or chemical methods. One widely used chemical is Ethyl Methane Sulphonate (EMS). Molecular techniques can detect genetic changes in mutated orchids early, without the need for the plants to mature. Thus, this study aimed to investigate genetic variation and detect mutants in D. bicaudatum orchids following EMS treatment using Sequence Related Amplified Polymorphism (SRAP). This study used four SRAP primer combinations. The DNA concentration of sample B2.Chimera approaches purity, indicated by an A160/280 ratio of 1.67. The results indicated that the four primers exhibited high orchid polymorphism, ranging from 91% to 100%. A total of 47 loci and 258 DNA bands were generated using four primers, yielding a PIC value of 0.32-0.37. The high level of polymorphism detected by SRAP indicates genetic diversity in mutant plants, which importantly contributes to conservation. The analysis of genetic similarity via dendrograms revealed two primary clusters at a coefficient of 0.23: one comprising albino explants and the other consisting of non-albino explants. This result suggests that albino mutants possess genetic traits that significantly differ from those of non-albinos. Non-albino explants, including chimeras and normals, exhibited high similarity coefficients ranging from 0.63 to 0.96.
First record and potential biocontrol implication of Beauveria bassiana infecting Lema yerburyi on Vanda tricolor from Bali, Indonesia I PUTU SUDIARTA; DEWA AYU PUTU KANIANITA SANJAYA; SHAH MAHAPATI DINARKAYA; GUSTI NGURAH ALIT SUSANTA WIRYA; I WAYAN DIKSA GARGITA; I KADEK WISMA YUDHA; IDA AYU PUTRI DARMAWATI; YUYUN FITRIANI; I MADE ARIMBAWA; DEWA GEDE WIRYANGGA SELANGGA; RINDANG DWIYANI; NOR KARTINI ABU BAKAR; YUSMIN MOHD-YUSUF
Biodiversitas Journal of Biological Diversity Vol. 26 No. 11 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261133

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Abstract. Sudiarta IP, Sanjaya DAPK, Dinarkaya SM, Wirya GNAS, Gargita IWD, Yudha IKW, Darmawati IAP, Fitriani Y, Arimbawa IM, Selangga DGW, Dwiyani R, Bakar NKA, Mohd-Yusuf Y. 2025. First record and potential biocontrol implication of Beauveria bassiana infecting Lema yerburyi on Vanda tricolor from Bali, Indonesia. Biodiversitas 26: 5755-5762. The application of the entomopathogenic fungus Beauveria sp. for biological control has emerged as a viable solution to the problem of excessive insecticide application. Morphological and molecular identification targeting the amplification range of 500-600 bp for entomopathogenic fungal rDNA and 700 bp for insect COI. Pathogenic tests through Koch's postulates, isolated and cultured using Potato Sucrose Agar (PSA) media. The results of the identification of the orchid beetle were identified as Lema yerburyi, and the common name "Orchid lema" was proposed to distinguish it from other orchid beetle pests. Macroscopic identification showed that the isolate Beauveria sp. has a white colony color, with a pale-yellow underside, aseptate hyphae and conidia, with conidia arranged in chains on conidiophores that show a zigzag pattern. Molecular analysis of Beauveria sp. isolates targeting the Internal Transcribed Spacer (ITS) region produced a DNA fragment of ~577 bp. Phylogenetic tree construction showed that this isolate grouped with B. bassiana from Malaysia. This study aimed to identify and confirm the entomopathogenic fungus naturally infecting L. yerburyi in Bali, and to evaluate its potential as a biological control agent. This finding expands the known host range of B. bassiana and provides a scientific basis for its potential application in Integrated Pest Management (IPM) programs for orchids. Future studies should evaluate field efficacy, optimize formulation, and assess its compatibility with existing cultural and biological practices to advance sustainable orchid production.
Co-Authors DEWA AYU PUTU KANIANITA SANJAYA DEWA GEDE WIRYANGGA SELANGGA FITRIA DWI DARMAYATI GEDE WIJANA GUSTI NGURAH ALIT SUSANTA WIRYA I KADEK WISMA YUDHA I MADE ARIMBAWA I PUTU SUDIARTA I WAYAN DIKSA GARGITA NI LUH MADE PRADNYAWATHI NOR KARTINI ABU BAKAR RINDANG DWIYANI RINDANG DWIYANI SHAH MAHAPATI DINARKAYA WAFA’ NUR HANIFAH YUSMIN MOHD-YUSUF YUYUN FITRIANI