PRABANG SETYONO
Bioscience Program, School of Graduates, Sebelas Maret University, Surakarta 57126, Central Java, Indonesia

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Alternative supplementary biochemic food for growing up the fresh water lobster (Cherax quadricarinatus) EDI PRIYONO; OKID PARAMA ASTIRIN; PRABANG SETYONO
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010304

Abstract

Priyono E, Astirin OP, Setyono P. 2009. Alternative supplementary biochemic food for growing up the fresh water lobster (Cherax quadricarinatus). Nusantara Bioscience 1: 123-130. This research denotes to know the influence of biochemic composition to the rapid grow of fresh water lobster on the stadium of post larva (PL) of 60 within three months. This research used the complete random planning dealing with 4 treatments and each treatment would get 3 times cycle. The treatments cover, group K tested animal was treted with 100% mill food containing 30% of protein. Group A is given with food and biochemic food containing 13,34% of protein. Group B is the treated with mill food which is mixed with biochemic food containing 10,7% of protein. While group C was tested by treating them with mill food and biochemic food containing 13,58%. After all the above mentioned would be set up within 3:1 comparation. The variable of this research were the length of the abdomen, cephalothorax, total length, and the wet weight. The data analysis is using ANOVA system on 95% power test completed by of SPSS version 13. The result of the research shows that mentioned treatments give us the same influence toward the growth of fresh water lobster. The composition of biochemic food with the containing protein around 10,7%,13.34% and 13,38% has given the same effect to the lobster growth on post larva 60 level. There is strong correlation between abdomen and cephalothorax and between the total length and the lobsters weight.
Variation of morphology, karyotype and protein band pattern of adenium (Adenium obesum) varieties DWI HASTUTI; SURANTO SURANTO; PRABANG SETYONO
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010205

Abstract

Hastuti D, Suranto, Setyono P. 2009. Variation of morphology, karyotype and protein band pattern of adenium (Adenium obesum) varieties. Nusantara Bioscience 1: 78-83. The aim of this research to find out the Adenium obesum variation from six varieties, namely: obesum, cery, red lucas, red fanta , white bigben and harry potter based on morphology, karyotype, as well as protein banding pattern. The chromosome preparation was made using semi-permanent squash method from the tip of root plant; while protein banding pattern was made using SDS-PAGE method. Qualitative data included shape and color of the leave and flower described from each variety. Data were presented in morphometry and analyzed using ANOVA and then followed by DMRT with 5% of confidence levels, indicated significance difference. Protein banding pattern, the root, stem, leave and all organs were analyzed using Hierarchical Cluster Analysis method with Average Linkage (between Groups) using SPSS 10.0. The result of research shows that the six A. obesum varieties have morphological character with no variation of light green to dark green leave, not hairy, smooth leave bone, meanwhile for light red to dark red flower crown color although some of them are white and the same funnel color, yellow. All varieties of A. obesum have same number of chromosome, 2n = 22 and shows the difference ranging from 2.56 to 5.13 μm. In the banding pattern formed qualitatively, there is variation among the six varieties.