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DARLINA MD NAIM
School of Biological Sciences, Universiti Sains Malaysia. 11800 Pulau Pinang, Malaysia

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Population genetic structure of Kawakawa (Euthynnus affinis Cantor, 1849) in Malaysian waters based on COI gene KHALED BINASHIKHBUBKR; AHMAD DWI SETYAWAN; DARLINA MD NAIM
Nusantara Bioscience Vol. 15 No. 2 (2023)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n150213

Abstract

Abstract. Binashikhbubkr K, Setyawan AD, Naim DM. 2023. Population genetic structure of Kawakawa (Euthynnus affinis (Cantor, 1849)) in Malaysian waters based on COI gene. Nusantara Bioscience 15: 258-268. Kawakawa (Euthynnus affinis Cantor, 1849) is widely distributed in the subtropical and tropical waters of the Indo-Pacific region. Still, insufficient data about its stock, management, and protection in Malaysia and nearby waters raises concerns about overfishing and depletion. Therefore, to ensure effective and successful management of a species, it is imperative to conduct a molecular-based assessment of the stock structure. The present study investigated the population genetic structure of E. affinis in Malaysian waters using the mtDNA COI gene. Furthermore, the 632 bp segment of the COI region was sequenced in 372 individuals from 19 distinct populations in Malaysian waters. The results revealed that the genetic divergence varied from low to high. The average Haplotype diversity (Hd) and nucleotide diversity (?) were calculated to be 0.5401 and 0.0045, respectively. Examining haplotype distribution unveiled the presence of 22 unique haplotypes within the COI gene of E. affinis. The analysis of the Neighbor Joining (NJ) tree and the Minimum Spanning Network (MSN) revealed the formation of three distinct clades among E. affinis samples. Analysis of Molecular Variance (AMOVA) showed a significant genetic structure among the 19 populations of E. affinis [(FST = 0.5354 (P < 0.05)]. The neutrality test and mismatch distribution analysis indicated that the specimens underwent a period of population expansion. This study is a significant milestone, providing the first comprehensive documentation of the genetic structure of E. affinis in Malaysia.
Morphometric variations of Kawakawa (Euthynnus affinis Cantor, 1849) in Malaysian and Yemeni waters KHALED BINASHIKHBUBKR; AHMAD DWI SETYAWAN; DARLINA MD NAIM
Nusantara Bioscience Vol. 15 No. 2 (2023)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n150214

Abstract

Abstract. Binashikhbubkr K, Setyawan AD, Naim DM. 2023. Morphometric variations of Kawakawa (Euthynnus affinis Cantor, 1849) in Malaysian and Yemeni waters. Nusantara Bioscience 15: 269-278. Euthynnus affinis Cantor, 1849 (Kawakawa), a small epipelagic tuna that migrates, is one of the most commercially significant tunas in Indo-Pacific subtropical and tropical waters. Unfortunately, there is a lack of knowledge on the region's stock structure and management of the particular migratory species. The present investigation aimed to distinguish body shape differences among 20 populations of E. affinis across Malaysian and Yemen coastal waters. A total of 416 specimens of E. affinis were sampled from five central geographic regions: the South China Sea, Straits of Malacca, Sulu Sea, Celebes Sea, and the Arabian Sea. Multivariate analyses, such as Principal Component Analysis (PCA), Discriminant Function Analysis (DFA), and Cluster Analysis (CA) of 12 morphometric variables, were conducted to differentiate 20 populations of E. affinis. The findings revealed significant differences in the body form among 20 populations. Morphological homogeneity was observed among populations from Peninsular Malaysia and Malaysian Borneo (Sabah and Sarawak). As expected, Yemen's (Mukalla City) populations were clearly segregated and formed a separate cluster. The highest percentage of correctly classified specimens (100%) came from populations in Pulau Tenggol (Terengganu) and Kukup (Johor). In contrast, the highest percentage of misclassified specimens (100%) came from populations in Kota Kinabalu (Sabah), Bintawa (Sarawak), Lumut (Perak), and Endau (Johor). Based on the Mahalanobis distances, hierarchical cluster analyses revealed that 20 populations of E. affinis could be clustered into three distinct groups. This is the first report on E. affinis from Peninsular Malaysia, Malaysian Borneo (Sabah and Sarawak), and the waters of Yemen using morphometric techniques.