Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Journal of Multidisciplinary Applied Natural Science
Mimi Sugiarti
Department of Medical Laboratory Technology, Poltekkes Kemenkes Tanjungkarang, Bandar Lampung-35145 (Indonesia)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Multiphase Nanoparticles from Eggshell Waste as Antibacterial Agents Against Rice Blight in Lampung Rodhiansyah Djayasinga; Wasinton Simanjuntak; Sumardi Sumardi; Sutopo Hadi; Mimi Sugiarti; Hartanti Hartanti; Rudy Tahan Mangapul Situmeang
Journal of Multidisciplinary Applied Natural Science Vol. 6 No. 3 (2026): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.384

Abstract

This study reports the first isolation of an Enterobacter cloacae complex (ECC) strain, closely related to Enterobacter hormaechei (99.86% 16S rRNA identity), associated with bacterial blight symptoms in rice in Lampung, Indonesia. Acknowledging 16S rRNA limitations within ECC, species identification is reported cautiously, while pathogenicity profile fulfilled Modified Koch's Postulates. To address chemical bactericide limitations, we developed sustainable multiphase nanoparticles from chicken eggshell waste through mechanical and thermal decomposition approaches. The produced nanoparticles were characterized using XRD, TEM, FTIR, SEM-EDS, PSA, and UV-DRS. The results showed that the nanoparticles consisting of Ca(OH)2, CaO, and β-tricalcium phosphate, with a dimension of 11–31 nm (XRD) and 20–23 nm (TEM). The FT-IR/EDS data confirmed OH⁻, Ca, O, and P elements, while UV-DRS showed a bandgap energy of 5.96 eV. Descriptive statistical analysis (n=6) evaluated antibacterial activity via disk diffusion (20 µg/disk) against the ECC isolate, compared to ampicillin sulbactam (SAM 20). Nanoparticles yielded a mean inhibition zone of 10.06±0.02 mm for responsive replicates, demonstrating moderate activity. Within the zones, bacterial cells showed morphological damage, likely caused by Ca²⁺ and OH⁻ release, inducing membrane disruption and oxidative stress. Furthermore, spatially graded element distribution (Ca, O, P) and β-TCP as a controlled release matrix support sustained bioactivity. These findings reveal eggshell-derived nanoparticles as a promising, economical, and environmentally friendly antibacterial agent for sustainable rice disease management.
Co-Authors Hartanti Hartanti Rodhiansyah Djayasinga Rudy Tahan Mangapul Situmeang Sumardi Sumardi Sutopo Hadi Wasinton Simanjuntak