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Laras Puspita Taib
Universitas Bina Mandiri Gorontalo
Health Professions Medicine & Pharmacology Nursing Public Health

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COMPARISON OF TOTAL CHOLESTEROL LEVELS IN SAMPLES SERUM TO BE CHECKED IMMEDIATELY AND SERUM SAMPLE WHICH WAS POSTPONED FOR 7 DAYS IN THE REFRIGERATOR WITH A TEMPERATURE OF 2-8°C AT BHAYANGKARA HOSPITAL Sri Miranda Maku; Muh. Rizman Naim; Laras Puspita Taib
Journal of Health, Technology and Science (JHTS) Vol. 7 No. 2 (2026): Journal of Health, Technology, and Science (JHTS)
Publisher : LPPM Universitas Bina Mandiri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47918/kdv97t04

Abstract

Total cholesterol measurement is an essential laboratory examination for assessing cardiovascular risk and monitoring lipid metabolism. However, immediate analysis is not always feasible in routine clinical practice, making refrigerated storage a common pre-analytical procedure. This study aimed to compare total cholesterol levels between serum samples analyzed immediately after collection and serum samples stored at 2–8°C for seven days. A quantitative analytical observational study with a repeated-measurement comparative design was conducted at Bhayangkara Hospital, Gorontalo, Indonesia, from June to July 2025. Fifteen paired serum samples were selected using accidental sampling. Total cholesterol concentrations were measured using the BioSystem BA200 automated chemistry analyzer. Differences between immediate and delayed analyses were evaluated using the paired-samples t-test after confirmation of data normality. Of the 15 samples, seven (47%) had normal cholesterol levels and eight (53%) had abnormal levels. The mean total cholesterol concentration increased slightly from 203 mg/dL to 206 mg/dL after seven days of refrigerated storage. However, the paired-samples t-test showed no statistically significant difference between the two conditions (p = 0.052). These findings indicate that serum storage at 2–8°C for up to seven days maintains total cholesterol stability and can be considered an acceptable pre-analytical practice when immediate laboratory analysis is not feasible.
THE EFFECT OF CIGARETTES ON RED BLOOD CELLS IN ACTIVE TEENAGE SMOKERS IN LEKOBALO VILLAGE, KOTA BARAT DISTRICT Laras Puspita Taib; Parhan Hanuna
Journal of Health, Technology and Science (JHTS) Vol. 6 No. 4 (2025): Journal of Health, Technology, and Science (JHTS)
Publisher : LPPM Universitas Bina Mandiri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47918/00ndev29

Abstract

Cigarette smoking during adolescence may affect hematological function through exposure to carbon monoxide, oxidative compounds, and other tobacco-related toxicants. This study aimed to determine the association between cigarette smoking frequency and red blood cell (RBC) values among active adolescent smokers in Lekobalo Village, Kota Barat District. A quantitative analytical observational study with a cross-sectional design was conducted in September 2025. The study involved 30 active smokers aged 15–19 years who consumed 3–10 cigarettes per day and were selected using random sampling. Smoking frequency was assessed through self-reported daily cigarette consumption, while RBC values were determined through laboratory examination of venous blood samples. Descriptive statistics and inferential analysis were performed, with statistical significance set at p < 0.05. Participants reported a mean smoking frequency of 6.13 ± 2.193 cigarettes per day, while the mean RBC concentration was 4.2020 ± 0.29847 million/µL, with values ranging from 3.70 to 4.76 million/µL. Inferential analysis demonstrated a statistically significant association between smoking frequency and RBC examination results (F = 11.623, p < 0.001). These findings indicate that variation in cigarette consumption was associated with variation in RBC values among adolescent smokers. However, the cross-sectional design does not establish a causal relationship. Early smoking prevention, cessation counseling, and appropriate hematological monitoring should be strengthened to reduce potential biological consequences of tobacco exposure among adolescents.
Co-Authors Muh. Rizman Naim Parhan Hanuna Sri Miranda Maku