Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal eJournal Kedokteran Indonesia
Raphael Salim
Indonesia International Institute for Life Science, Jakarta, Indonesia
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
Brief Report: Evaluation of Ziehl-Neelsen Slide Restaining with 0.4% Malachite Green for Identifying Cyclospora cayetanensis Oocysts Sri Wahdini; Raphael Salim; Ika Puspa Sari; Agnes Kurniawan
eJournal Kedokteran Indonesia Vol. 13 No. 3 (2025): Vol. 13 No. 3 - Desember 2025
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23886/ejki.13.1249.1

Abstract

Cyclospora cayetanensis oocysts are commonly identified using Ziehl-Neelsen acid-fast staining. However, slides stored for seveal periods often exhibit color fading, reducing visual clarity and limiting their suitability for training and educational purposes. This study evaluated the effectiveness of restaining with 0.4% malachite green to enhance contrast and visualization of oocysts on faded slides. A quantitative experimental laboratory design was employed. Ten positive slides stored at the Clinical Parasitology Laboratory, Faculty of Medicine Universitas Indonesia, were selected based on the degree of fading and staining thickness. Each slide was restained with 0.4% malachite green for 30 seconds after destaining with methanol. Following drying, slides were examined under a light microscope, and oocysts were manually counted. Restaining restored the background to a bluish-green hue, improving visual contrast and facilitating oocyst identification. Restaining Ziehl-Neelsen slides with 0.4% malachite green is a simple and effective method for enhancing visualization and identification of C. cayetanensis oocysts.
Co-Authors Agnes Kurniawan Ika Puspa Sari Sri Wahdini