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All Journal PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Journal of Pharmaceutical and Sciences.
Yunita Al Azzahra
Akademi Farmasi Bumi Siliwangi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Analisis Kadar Alkaloid Ekstrak Dan Fraksi Biji Trembesi (Samanea saman Merr.) Berdasarkan Kepolaran Pelarut Menggunakan Spektrofotometri UV-Vis Raihan Ahista Qolbi; Yunita Al Azzahra
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1101

Abstract

Samanea saman (Merr.) seeds are plant parts that have not been extensively studied, despite their potential as a source of bioactive compounds, particularly alkaloids. Alkaloid compounds are known to possess various pharmacological activities, such as antimicrobial, anticancer, and analgesic effects, making them promising candidates for the development of natural medicines. This study aimed to analyze the alkaloid content in the extract and fractions of S. saman seeds using solvents with different polarity levels,namely n-hexane (non-polar), ethyl acetate (semi-polar), and distilled water (polar). Extraction was carried out using the Ultrasound Assisted Extraction (UAE) method, while quantitative analysis of alkaloid content was performed using UV-Vis spectrophotometry with caffeine as the standard solution. Qualitative testing showed that the ethyl acetate and aqueous fractions were positive for alkaloids. Quantitative analysis of the extract revealed that S. saman seed extract contained alkaloids at a concentration of 142.59 mgCE/g extract. Further analysis of the fractions indicated that the ethyl acetate fraction had the highest alkaloid content 77.34 mgCE/g fraction, compared to the aqueous fraction 24.65 mgCE/g fraction and the n-hexane fraction 45.7 mgCE/g fraction. These findings suggest that semi-polar solvents are the most effective for extracting alkaloid compounds from S. saman seeds, thereby highlighting their potential for further development as raw materials for phytopharmaceuticals or natural-based herbal preparations.
Analisis Kadar Flavonoid Total Ekstrak dan Fraksi Biji Trembesi (Samanea saman (Jacq.) Merr) Menggunakan Metode Spektrofotometri UV-Vis Yunita Al Azzahra; Mila Warmila
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 23 No. 01 Juli 2026
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

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Abstract

Salah satu senyawa metabolit sekunder yang banyak ditemukan pada berbagai bagian tumbuhan adalah flavonoid. Trembesi (Samanea saman (Jacq.) Merr) merupakan tanaman yang mudah dijumpai di Indonesia dan kerap digunakan sebagai pohon pelindung yang memainkan peran penting dalam ekosistem lokal. Namun, pemanfaatan biji trembesi masih sangat terbatas karena sering dianggap sebagai limbah. Hasil uji kualitatif menunjukkan bahwa biji trembesi mengandung senyawa aktif seperti alkaloid, saponin, dan flavonoid. Penelitian ini bertujuan untuk menganalisis kadar flavonoid total pada ekstrak dan fraksi biji trembesi. Metode yang digunakan dalam penelitian ini meliputi uji kualitatif dan uji kuantitatif. Uji kualitatif dilakukan dengan menggunakan pereaksi serbuk Mg dan HCl pekat, yang menunjukkan hasil positif terhadap keberadaan flavonoid. Sementara itu, uji kuantitatif dilakukan menggunakan spektrofotometri UV-Vis dengan standar kuersetin. Hasil penelitian menunjukkan bahwa kandungan total dalam ekstrak biji trembesi adalah 84,924 ± 0,008 mg QE/g, sedangkan fraksi etil asetat mengandung 13,909 ± 0,273 mg QE/g, fraksi air 1,295 ± 0,061 mg QE/g dan fraksi n-heksana 0,234 ± 0,007 mg QE/g.
Co-Authors Mila Warmila Raihan Ahista Qolbi