Jambi City Regional Regulation Number 2 of 2014 concerning the Eradication of Prostitution and Immoral Acts is implemented as an effort by the local government to suppress the practice of prostitution, which is considered a form of social pathology. This policy has had a significant impact, including the closure of the two largest localizations in Jambi City, namely Payo Sigadung (Pucuk) and Langit Biru. However, its effectiveness is still debatable because the practice of prostitution still occurs covertly in various places. This study aims to juridically analyze the substance and implementation of Jambi City Regional Regulation Number 2 of 2014 and to assess its effectiveness in preventing social pathologies. The research method used is qualitative with an exploratory approach. The data used are secondary sources from legal documents, online media, and relevant scientific journals. Data analysis was conducted using NVivo 12 Plus to explore themes, word frequencies, and relationships among legal concepts related to the implementation of local regulations. The results of the study show that this regulation has a strong legal basis and has contributed to suppressing the practice of prostitution administratively. However, its implementation still faces various obstacles such as weak legal sanctions, limited inter-agency coordination, and suboptimal rehabilitation and economic empowerment programs for former sex workers. Juridically, Regional Regulation Number 2 of 2014 plays a vital role in preventing social pathologies. However, it still requires a more comprehensive legal approach and synergy with social, economic, and cultural strategies to create a sustainable moral order in Jambi City.