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Influences of bull age and season on sperm motility, sperm concentration, and ejaculate volume of Ongole Grade cattle in Singosari National Artificial Insemination Center Suyadi, S.; Purwantara, B.; Furqon, A.; Septian, W. A.; Novianti, I.; Nursita, I. W.; Nugraha, C. D.; Putri, R. F.; Pratiwi, H.; Herwiyati, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 4 (2020): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.4.261-267

Abstract

Ongole Grade cattle is one of local cattle in Indonesia.This study was aimed to analyze the effect of bull age and season on semen quality of Ongole Grade cattles macroscopically (sperm motility, and sperm concentration) and microscopically (semen ejaculate volume) to support the policy and management on improvement of semen production. Data were collected from 533 ejaculates of 10 bulls aged 3, 4, and 5 yr at Singosari National Artificial Insemination Center(SNAIC). The 4-year-old bulls hadhighestsperm motiliy and semen ejaculate volume. The lowest ejaculate volume was reported in Ongole Gradecattlesaged 3 yr. Sperm motility and concentrationweresignificantly higher (P<0.01) in wet season than dry season. In conclusion, the bull age and season should be considered by Singosari National Artificial Insemination Centerwhen collecting the semen, especially on bulls aged 3 yr old and in dry season.
Correlations between age of Bali bull and semen production at National Artificial Insemination Center, Singosari - Indonesia Nugraha, C. D.; Herwijanti, E.; Novianti, I.; Furqon, A.; Septian, W. A.; Busono, W.; Suyadi, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.3.258-265

Abstract

The aim of this research was to analyze the correlation of age of Bali bull to the body weight and semen qualities. The materials were 50 Balibulls data collected from 2012 to 2018. Therecordeddatawere consisted ofsemen productions (n = 1304) and body weight (n = 1544). The observation was conducted to analysis the interrelationship between the Bali bulls age and body weight (BW), to the semen volume (SV), individual motility (IM) and sperm concentration (SC). The obtained data was analyzed with One-Way ANOVA analysisby using SPSS 24 and followed with Duncan’s Multiple Range Test to determine any significant differences. The correlation between Bali bulls age (the independent variable) and semen quality (the dependent variable) was computed by using Pearson correlation method and presented in linear regression models. The results showed that the Bali bulls agesignificantly affect (P<0.05) the BW from the age of 1 to 10 years old. The age also showed a significant effect (P<0.05) on the semen qualities. The age correlated significantly (P<0.05) with BW, SV, IM, SC were 0.658, 0.386, 0.134 and 0.086, respectively. Inconclusion,the age of Bali bull could be used as an indicator todetermineits BWand semenqualities. 
The estimation of semen production based on body weight and scrotal circumference on PO Bull at Singosari National Artificial Insemination Center Muthiapriani, L.; Herwijanti, E.; Novianti, I.; Furqon, A.; Septian, W. A.; Suyadi, Suyadi
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 29, No 1 (2019): April
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2019.029.01.09

Abstract

Ongole Crossbred cattle or more popular known as Peranakan Ongole (PO) cattle is one of local breeds cattle in Indonesia. For improving the population and genetic quality, superior bull with high capacity to produce semen is seriously required. This study aimed to find out the estimation value of semen production of PO bull based on body weight and scrotal circumference. A total sampling of 9 bull candidates of PO cattle available at National Artificial Center (NAIC) Singosari, Malang, Indonesia were used in this study. The method used was case study. Scrotal circumference was directly measured as a primary data. While body weight, semen volume, semen concentration and sperm motility were collected from BBIB Singosari from secondary data. Data was analyzed using simple linear regression. The results showed that with the limited number of samples, there is no significant correlation (P>0.05) between body weight and scrotal circumference on semen volume, semen concentration, and sperm motility.  It was concluded that with limited number of samples involved in this study, there was no significantly on semen production based on body weight and scrotal circumference on PO bull. The suggestion was to improve the correlation value and significant result. The number of samples should be increased.
Expression and Association of SCD Gene Polymorphisms and Fatty Acid Compositions in Chicken Cross Furqon, A.; Gunawan, A.; Ulupi, N.; Suryati, T.; Sumantri, C.
Media Peternakan Vol. 40 No. 3 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (666.827 KB) | DOI: 10.5398/medpet.2017.40.3.151

Abstract

Stearoyl-CoA desaturase (SCD) is an integral membrane protein of endoplasmic reticulum (ER) that catalyzes the rate limiting step in the monounsaturated fatty acids from saturated fatty acids. Selection for fatty acids traits based on molecular marker assisted selection is needed to increase a value of chicken meat. This study was designed to analyze expression and associations of SCD gene polymorphisms with fatty acid traits in F2 kampung-broiler chicken cross. A total of 62 F2 kampung-broiler chicken cross (29 males and 33 females) were used in this study. Fatty acid traits were measured at 26 weeks of age. Samples were divided into two groups based on fatty acid traits (the highest and the lowest). Primers in exon 2 region were designed from the genomic chicken sequence. The SNP g.37284A>G was detected and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was then used to genotype. The expression of SCD gene was analyzed using quantitative real time PCR (qRT-PCR). The result showed that there were three genotypes (AA, AG, and GG) found in this study. The SCD|AciI polymorphism was significantly associated with palmitoleic acid (C16:1), fatty acids total and saturated fatty acid in 26 weeks old of F2 kampung-broiler chicken cross (P<0.05). The SCD gene was expressed for polyunsaturated fatty acids in liver tissue in two groups of chickens. In conclusion, the SCD gene could be a candidate gene that affects fatty acids traits in F2 kampung-broiler chicken cross.
Deteksi Kandungan Gelatin Babi pada Produk Permen Jelly Menggunakan Penada Gen Sitokrom B Nuraini, H.; Furqon, A.; Sari, S. A.; Dewi, M. K.; Zainatha, F. M.; Sumantri, C.
Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan Vol. 1 No. 2 (2013): Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan
Publisher : Department of Animal Production and Technology, Faculty of Animal Science, IPB University in associated with Animal Scientist's Society of Indonesia (HILPI)

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Abstract