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Penyimpanan Serbuk Sari Jagung dan Potensinya untuk Produksi Benih Hibrida Fariroh, Indri; Palupi, Endah Retno; Suwarno, dan Faiza Chairani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 2 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.285 KB) | DOI: 10.24831/jai.v45i2.12535

Abstract

Production of maize hybrid seed is hindered by relatively poor production by the male line pollen; short live of the pollen which was ascribed to desiccation and dehydration sensitive and flowering asynchrony between male and female lines. The aims of this study were to enhance production and viability of male line pollen and also to determine the suitable storage condition for maintaining pollen viability in order to be used for production of hybrid seed. The research consisted of two experiments. The first experiment was arranged in split plot randomized block design with NPK dosages as main plot (0, 300, 600, 900 kg ha-1) and application of boron as sub-plot (0, 1.5, 3, 4.5, 6 kg ha-1), replicated three times. The second experiment used randomized block design with two factors, i.e. storage temperature (-196 oC and -20 oC) and storage period (0, 1, 2, 3, 4 weeks). The result showed that NPK 900 kg ha-1 increased the number of spike per tassel from 9.3 to 10.7. Application of NPK 600 kg ha-1 or boron 1.5 kg ha-1 improved pollen viability. Combination of NPK 900 kg ha-1 and boron 6 kg ha-1 resulted in the highest pollen viability of 95.3%. Pollen after being stored for four weeks either in -196 ºC or -20 ºC was possible to be used for seed production with 20% seed set.Keywords: boron, freezer, liquid nitrogen, NPK dosage, pollen viability  
Perilaku Biokimia Benih Kedelai Selama Penyimpanan dalam Kondisi Terkontrol Noviana, Irma; Qadir, Abdul; Suwarno, dan Faiza Chairani
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 44 No. 3 (2016): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.751 KB) | DOI: 10.24831/jai.v44i3.12931

Abstract

ABSTRACTPhysiological and biochemical changes in seeds indicates levels of viability and vigor during deterioration. The aims of the research were to study the patterns of biochemical changes in soybean seeds and its relationship to seed vigor during storage under controlled conditions. The experiment was arranged in a completely randomized design using two varieties as a factor which was nested into storage period and replicated four times. Two varieties were Gepak Kuning and Mallika, stored in controlled condition at temperature of 19-22 °C and 64-67% of relative humidity for six months. The seeds were evaluated for protein, peroxide value, electrical conductivity and seed viability. The exponential regression analysis was used to determine the patterns of biochemical changes of each soybean varieties during the storage period. The results showed that the biochemical behaviour of soybean seeds stored under controlled conditions for 24 weeks following the exponential equation of y = a + b expcx, which a, b, and c were constants that has different value for each variety. Electrical conductivity and peroxide value could be used as indicators of seed vigor during storage under controlled conditions for 24 weeks. Seed storage under controlled conditions are to maintain seed vigor >80% up to 12 weeks for Mallika and 24 weeks for Gepak Kuning with low oxidative process and less membrane damages.Keywords:deterioration, storability, vigor