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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) AGRIVITA, Journal of Agricultural Science
Elina, Juanita
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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Clonal Fidelity of Micro-propagated Phalaenopsis Plantlets Based on Assessment Using Eighteen Ph-Pto SNAP Marker Loci Raynalta, Erick; Elina, Juanita; Sudarsono, Sudarsono; Sukma, Dewi
AGRIVITA, Journal of Agricultural Science Vol 40, No 3 (2018): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i3.1493

Abstract

Phalaenopsis amabilis is an Indonesia native orchid species having large, white flowers with yellow labellum coloration. This studies aimed to develop Phal. amabilis micropropagation methods and evaluate the regenerated plantlet fidelity. Media supplemented with Thidiazuron (TDZ) and Polyvinylpyrrolidone (PVP) and medium pH adjustment effects to induce protocorm-like bodies (PLBs) from leaf explants and proliferate secondary PLBs were investigated. Clonal fidelity among regenerated plantlets was evaluated using eighteen SNAP marker loci. The results showed that the ½ MS medium supplemented with 3 mg L-1 TDZ and 0.5 g L-1 PVP was the best for PLB induction while the ½ MS medium supplemented with 0.5 mg L-1 TDZ was the best for PLB proliferation. For PLB induction, the media pH was adjusted into pH=7 for efficient PLB regeneration. Based on the assessment using 18 SNAP marker loci, four variant alleles in three loci (11.8%) out of a total 34 plantlets were detected. The mutation frequency at the evaluated SNAP marker loci was 2.5 x 103 (0.25%). Changes in SNP alleles may not always result in phenotype changes and allele variant occurrences may not affect phenotype fidelity of micro-propagated Phal. amabilis plantlets. Therefore, further studies about the phenotype fidelity among plantlets are necessary.
Isolasi dan Karakterisasi Gen Pto Asal 20 Aksesi Anggrek Phalaenopsis Elina, Juanita; Sukma, Dewi; ,, Giyanto; sudarsono, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 2 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (970.136 KB) | DOI: 10.24831/jai.v45i2.12905

Abstract

Bacterial soft rot disease because of Dickeya sp. infection is the main problem in Phalaenopsis production in Indonesia, but the percentage of infected plants has never been recorded in detail. Isolation and characterization of Pto gene from Phalaenopsis could be useful to support breeding for resistance Phalaenopsis. Encoding serine-threonine kinase, Pto gene confers resistance to bacterial infection of Pseudomonas syringae in tomato. The objectives of this study were to isolate, sequence and characterize fragment of Pto gene from 20 genotypes of Phalaenopsis (16 species and 4 hybrids) and to evaluate their molecular diversity. Genomic fragments of Phalaenopsis were amplified using Pto specific degenerate primers; and the PCR amplicons were sequenced. Searching the identity of determined sequences was done using BLAST against all accessions in NCBI GenBank DNA database and in Conserve Domain Database. PCR amplification using Pto specific primers produced a single DNA fragment of ~500 bp. The determined nucleotide sequences from the amplicon were ~449 bp. The nucleotide sequences of the amplicons from 20 Phalaenopsis genotypes showed high sequence identity to Pto from Musa acuminata. Translation of the amplicon results in ~149 amino acid residues. Comparison of the translated polypeptides identify indicated there were low variations of Pto gene among accessions since they contain the PTO catalytic domain and the Serine/Threonine kinases, sub family of Interleukin-1 Receptor Associated Kinase (STK_IRAK) which are the conserved domains for PTO.Keywords: catalytic domain, disease resistance, fragment Pto gene, phylogenetic, RGA
Co-Authors ,, Giyanto Dewi Sukma Erick Raynalta, Erick Sudarsono Sudarsono, dan