Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal JURNAL BIOLOGI INDONESIA Indonesian Journal of Jamu
Suparto, Irma H
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
IDENTIFIKASI MOLEKULER VIRUS PAPILLOMA GENITAL PADA DUA SPESIES PRIMATA DI FASILITAS PENANGKARAN PUSAT STUDI SATWA PRIMATA-INSTITUT PERTANIAN BOGOR Sari, Isti Kartika; Suparto, Irma H; Iskandriati, Diah
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.339

Abstract

Tulisan Pendek
Cytotoxic and Antiproliferative Activities of Melia azedarach Leaves Ethanolic Extract on A549 Human Lung Cancer Cells Irshan, Yusuf; Suparto, Irma H; Sulistiyani, Sulistiyani
Jurnal Jamu Indonesia Vol. 2 No. 1 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v2i1.26

Abstract

Melia azedarach (Meliaceae) is known locally as mindi, gringging, renceh, or cakra-cikri is known to have pharmacological properties. The leaves contain flavonoids such as kaempferol and quercetin that have anticancer activity. The objective of this research is to evaluate the potency of Melia azedarach leaves ethanolic extract as anticancer by inhibition of cancer cell proliferation. Cytotoxic effect was analyzed by Brine Shrimp Lethality Test (BSLT) and cell viability (MTT) methods using Chang human normal liver cells and A549 human lung cancer cells. Antiproliferative effect of the extract was analyzed by cell direct calculation method using hemacytometer. The leaves were extracted with ethanol 96% by maceration method. Phytochemical investigation showed that the extract contains flavonoids, alkaloids, tannins, saponins, and steroids. Assay of BSLT showed that crude ethanolic extract of M. azedarach has a cytotoxic effect with LC50 value of 63.98 µg/mL, which is an indication for very potential bioactive compund as anticancer. Consistent with BSLT assay, cell viability (MTT) assay showed that this extract was able to reduce cell viability with IC50 values of 299.22 µg/mL on Chang cells and 130.56 µg/mL on A549 cancer cells. As control, Curcuma zedoaria extract at 75 µg/mL reduced A549 cells viability to 38.8%, which was equivalent to the effect of M. azedarach extract at 50 µg/mL. These data suggest that Melia azedarach extract is potentially more bioactive than Curcuma zedoaria extract. This result is supported by the ability of the extract (5 µg/mL) to inhibit A549 cells proliferation as much as 73.53%.
Co-Authors DIAH ISKANDRIATI Sari, Isti Kartika SULISTIYANI SULISTIYANI Yusuf Irshan