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All Journal Program Kreativitas Mahasiswa - Penelitian Indonesian Journal of Jamu
Yusuf Irshan
Biokimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Medicine & Pharmacology

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POTENSI PERMEN TIRAMISU YANG BERKHASIAT ANTIKOLESTEROL Waliyuddin, Waliyuddin; Yunita, Elvira; Karomah, Nailatul; Irshan, Yusuf; Amellia, Amellia
Program Kreativitas Mahasiswa - Penelitian PKM-P 2013
Publisher : Ditlitabmas, Ditjen DIKTI, Kemdikbud RI

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Abstract

Tiramisu candy is made of oyster mushroom extract ethanol. Oyster mushroom (Pleurotus ostreatus) contains lovastatin that inhibit cholesterol synthesis in the body. Ethanol extract of oyster mushroom was expected to give an effect in lowering cholesterol. This experiment used 35 male Sprague dawley rats that were divided into 5 groups (n=7).  The treatment period was for 14 days, normal group fed by standard woof, hypercholesterolemic group (HK) were fed by high- cholesterol woof (3%) and PTU (0.5 mg/kg BW), the group of lovastatin(lovastatin+HK0.2857mg/kgBW), treatment I group (HK+ oyster mushroom ethanol extract 30 mg/kg BW), and treatment II group (HK+ oyster mushroom ethanol extract 60 mg/kg BW). Blood was taken through the eyes of rats with hematocrit-heparin. Candy is made with a mixture of 5% starch, maltodextrin 10%, 4% magnesium stearate, sucrose 81%, and ethanol extract 2.1 grams of white oyster mushroom.The results showed that the ethanol extract of oyster mushroom (30 mg/kg BW and 60 mg/kg BW ) may decrease 53.89%  and 66.43% of cholesterol concentration. AST and ALT results of the analysis showed that the ethanol extract of white oyster mushroom dose of 30mg/ kg and 60 mg/ kg did not cause liver damage. Based on HPLC analysis,  a gram of oyster mushroom ethanol extract containing 3.6x10- 6 grams of lovastatin. Tiramisu candy successfully made by weight 100 mg to extract content of 2.1 mg and 7.56x10-6mg of lovastatin. Keywords : AST ALT, candy, cholesterol, lovastatin, Pleurotus ostreatus.
Cytotoxic and Antiproliferative Activities of Melia azedarach Leaves Ethanolic Extract on A549 Human Lung Cancer Cells Irshan, Yusuf; Suparto, Irma H; Sulistiyani, Sulistiyani
Jurnal Jamu Indonesia Vol. 2 No. 1 (2017): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v2i1.26

Abstract

Melia azedarach (Meliaceae) is known locally as mindi, gringging, renceh, or cakra-cikri is known to have pharmacological properties. The leaves contain flavonoids such as kaempferol and quercetin that have anticancer activity. The objective of this research is to evaluate the potency of Melia azedarach leaves ethanolic extract as anticancer by inhibition of cancer cell proliferation. Cytotoxic effect was analyzed by Brine Shrimp Lethality Test (BSLT) and cell viability (MTT) methods using Chang human normal liver cells and A549 human lung cancer cells. Antiproliferative effect of the extract was analyzed by cell direct calculation method using hemacytometer. The leaves were extracted with ethanol 96% by maceration method. Phytochemical investigation showed that the extract contains flavonoids, alkaloids, tannins, saponins, and steroids. Assay of BSLT showed that crude ethanolic extract of M. azedarach has a cytotoxic effect with LC50 value of 63.98 µg/mL, which is an indication for very potential bioactive compund as anticancer. Consistent with BSLT assay, cell viability (MTT) assay showed that this extract was able to reduce cell viability with IC50 values of 299.22 µg/mL on Chang cells and 130.56 µg/mL on A549 cancer cells. As control, Curcuma zedoaria extract at 75 µg/mL reduced A549 cells viability to 38.8%, which was equivalent to the effect of M. azedarach extract at 50 µg/mL. These data suggest that Melia azedarach extract is potentially more bioactive than Curcuma zedoaria extract. This result is supported by the ability of the extract (5 µg/mL) to inhibit A549 cells proliferation as much as 73.53%.
Co-Authors Amellia Amellia Elvira Yunita Nailatul Karomah SULISTIYANI SULISTIYANI Suparto, Irma H Waliyuddin Waliyuddin