Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Jurnal Teknologi Laboratorium Makara Journal of Science JOURNAL OF CHEMICAL NATURAL RESOURCES (JCNaR)
Amanda, Hilda
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Other

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Aktivitas Anti-Inflamasi Senyawa Squale dari Ekstrak Metanol Abroma augusta L pada Mus musculus Latief, Madyawati; Muhaimin, Muhaimin; Amanda, Hilda; Prahandika, Graha; Tarigan, Indra Lasmana
Jurnal Teknologi Laboratorium Vol 9 No 2 (2020): 2020 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i2.228

Abstract

Abroma augusta L plant traditionally was used to treat swellings, cuts, sores, and bruises. In the province of Jambi, A. augusta is used in folk medicine to treat wounds. This study aims to isolate the steroid compound from the root of A. augusta L and determine its anti-inflammatory activities. Extraction and fractionation have been done with graded maceration using solvents with different polarities, which are n-hexane, ethyl acetate, and methanol. The separation was performed by column chromatography, followed by preparative thin-layer chromatography. The characterization of the isolate was carried out using UV-Vis spectrophotometry and infrared spectrophotometry, GC-MS. The anti-inflammatory activities of methanol extract and isolate of A. augusta was performed in this study was designed to evaluate the dose-response relationship of the anti-inflammatory activity in rat models of chronic inflammation chromatography to obtain isolate 2.1.1 that characterize and showed maximum absorbance at 265. The result of IR showed the presence of functional groups, -C=C-H, -C=H, -CH, CH3, CH2, and –CO belongs to the steroid compound. The results of the GC-MS show that isolates contain squalene compounds with a value of m/z 410, Isolate and crude extract showed an anti-inflammatory activity that almost approached the positive control of sodium 4-chlorophenolate. It could be concluded that isolate and extract provide good anti-inflammatory activity, that promise for new drug candidate squalene-based A. augusta.
Isolation and Characterization of Ethyl Acetate Fraction from Abroma augusta L as an Anti-Inflammatory Agent Latief, Madyawati; Tarigan, Indra Lasmana; Muhaimin, Muhaimin; Amanda, Hilda; Yulianti, Nike Desvi
Makara Journal of Science Vol. 25, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abroma augusta is a bush plant that lives on the edge of the river. This plant is commonly used as an anti-inflammatory drug for joints and broken bones. It contains several secondary metabolites, such as alkaloids, triterpenoids, steroids, and flavonoids, which exhibit anti-inflammatory activity. UV-visible (UV-Vis) spectrophotometry of isolate 1.3 indicated absorption at a maximum wavelength of 282 nm. The wavelength suggested that the electron transition π–π* is the absorption of UV spectra typical for triterpenoid compounds that have chromophores in the form of non-conjugated double bonds. FT-IR spectrophotometer characterization data from isolate 1.3 revealed the presence of triterpenoid compounds having carboxyl groups C-O (ester), C-(CH3)2 (gem-dimethyl), carbonyl C = O esters, and C-H (alkyl). Analyses of UV-Vis, FT-IR, GC-MS, 1H-NMR, and 13C-NMR spectra showed that the compound obtained was the triterpenoid β-amyrin. Activity test demonstrated that isolate 1.3 with a 10 mg dose showed stronger anti-inflammatory activity than the positive control of sodium diclofenac.
The Isolation and Characterization of Phenolic Compound of Euphorbia Plant/Patikan Cina (Euphorbia thymifolia Linn) Amanda, Hilda
Journal of Chemical Natural Resources Vol. 1 No. 1 (2019): Journal of Chemical Natural Resources
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (726.65 KB) | DOI: 10.32734/jcnar.v1i1.833

Abstract

Euphorbia plant, Euphorbia thymifolia Linn, is one the Euphorbiaceae families that still need to be developed due to its benefits. An effort to be able to use this plant maximumly is by studying the active component in the plant. This study aims to isolate and characterize phenolic compound in euphorbia plant extract using ethyl acetate. 500 gram of euphorbia plant (Patikan Cina) powder was macerated using methanol. Then, the macerated extract was evaporated to eliminate excess solvent and as a result, a solvent-free extract was obtained. Next, the result was partitioned with a solution of methanol : ethyl acetate (1:1) in order to get methanol and ethyl acetate extracts. The ethyl acetate extract of the partition was evaporated to get concentrated ethyl acetate extract. Next, it was isolated in a vacuum liquid and gravitational column chromatography to get pure isolate. Silica gel 60 (0.040 – 0.063 mm) and n-hexane motion phase were used in the separation of concentrated ethyl acetate in vacuum liquid chromatography. There were 16 fractions produced from the yield. Then the fractions were put in TLC. With silica gel 60 (0.2 – 0.5 mm) mesh and n-hexane mobile phase, ethyl acetate gave 5 fractions in gravitational column chromatography and fraction 3 produced a single spot. UV and IR spectroscopy were used to determine the constituents in the isolate. From UV spectrum, max is 268.97 nm. While IR data shows the presence O-H group in 3521.38 cm-1, C – H (alkane) presents at wave number 2926.45 cm-1 reinforced by the appearance of 5 other alkanes absorption at 2857.00 cm-1, 1447.31 cm-1, 1370.18 cm-1, 868.774 cm-1 and 757.887 cm-1 wavelengths. There is also C = O (carbonyl) groups in wave number 1693.19 cm-1. The identification of structures based on UV and IR spectra data has shown that the isolate is a phenolic compound.
Co-Authors Indra Lasmana Tarigan Madyawati Latief Muhaimin Muhaimin Prahandika, Graha Yulianti, Nike Desvi