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All Journal KESANS : International Journal of Health and Science
Tiwi Yuniastuti
Environmental Health Study Program, STIKES Widyagama Husada Malang, Indonesia
Chemistry Health Professions Medicine & Pharmacology Physics Public Health

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The Effectiveness of Butterfly Pea (Clitoria ternatea L.) and Golden Trumpet (Allamanda cathartica) Flower as Natural Larvicides Against Aedes aegypti Nayla Mahdiyafada Santoso; Tiwi Yuniastuti; Irfany Rupiwardani
Jurnal KESANS : Kesehatan dan Sains Vol 5 No 11 (2026): KESANS: International Journal of Health and Science
Publisher : Rifa'Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54543/kesans.v5i11.715

Abstract

Introduction: Aedes aegypti is a major dengue vector, requiring alternative plant-based larvicides. Objective: This study compared the larvicidal effectiveness of butterfly pea (Clitoria ternatea L.) and golden trumpet (Allamanda cathartica) flower extracts against Aedes aegypti larvae. Method: A true experimental study with a completely randomized design (CRD) was conducted using butterfly pea and golden trumpet flower extracts prepared by maceration with distilled water at concentrations of 15%, 25%, 35%, and 45%, with three replications per treatment. Twenty-five third- to fourth-instar Aedes aegypti larvae were exposed to each treatment, and mortality was assessed after 24 hours. Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Results and Discussion: Butterfly pea extract produced mortality of 38%, 58%, 50%, and 61%, while golden trumpet extract produced 17%, 34%, 37%, and 46%, respectively. Butterfly pea showed higher mortality at all concentrations. The Kruskal-Wallis test showed significant differences among concentrations for both extracts (p = 0.018 and p = 0.010). However, the Mann-Whitney test showed a significant difference between extracts only at 25% concentration (p = 0.043). Conclusion: Both extracts exhibited larvicidal activity, with butterfly pea showing higher effectiveness descriptively
Analysis of Organophosphate Pesticide use and Cholinesterase Enzyme Levels Among Farmers in Banjarejo Village, Pakis District M Gibran Deva Primartha; Beni Hari Susanto; Tiwi Yuniastuti
Jurnal KESANS : Kesehatan dan Sains Vol 5 No 12 (2026): KESANS: International Journal of Health and Science
Publisher : Rifa'Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54543/kesans.v5i12.722

Abstract

Introduction: Organophosphate pesticides are widely used to control agricultural pests and may inhibit cholinesterase activity, making blood cholinesterase a useful biomarker of exposure. Objective: This study analyzed the use of organophosphate pesticides and cholinesterase levels among farmers in Banjarejo Village, Pakis District, Malang Regency. Method: This observational analytic study used a cross-sectional design. Nine active pesticide-spraying farmers were selected by purposive sampling. Data on age, sex, knowledge, spraying frequency and duration, personal protective equipment (PPE), working period, and pesticide dose were collected using questionnaires and observation. Cholinesterase levels were examined from blood samples. Fisher's Exact Test was used for bivariate analysis with α=0.05. Results and Discussion: Seven respondents (77.8%) had normal cholinesterase levels and two (22.2%) had abnormal levels. Fisher's Exact Test showed no significant association between sex (p=1.000), knowledge (p=0.444), spraying frequency (p=1.000), working period (p=0.444), and pesticide dose (p=1.000) with cholinesterase levels. Age, spraying duration, and PPE use could not be statistically tested because their categories were constant. Conclusion: Most respondents had normal cholinesterase levels, but abnormal results were still found. The small sample and limited variation among exposure categories restrict statistical interpretation. Safe pesticide handling, correct dosing, and complete PPE use should nevertheless be strengthened
Co-Authors Beni Hari Susanto Irfany Rupiwardani M Gibran Deva Primartha Nayla Mahdiyafada Santoso