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All Journal Jurnal Teknologi Pertanian Jurnal Pengabdian UNDIKMA
Rukmi, Widya Dwi
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POTENSI BAKTERI LAUT SEBAGAI SUMBER ANTIBIOTIK BARU PENGHAMBAT STAPHYLOCCUS AUREUS Mardiana, Nur Agustin; Murniasih, Tutik; Rukmi, Widya Dwi; Kusnadi, Joni
Jurnal Teknologi Pertanian Vol 21, No 1 (2020)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.714 KB) | DOI: 10.21776/ub.jtp.2020.021.01.6

Abstract

ABSTRAK Penemuan akan antibiotik baru yang semakin menurun dan meningkatnya penyakit yang disebabkan oleh Staphylococcus aureus  telah meningkatkan minat terhadap bakteri laut sebagai produsen senyawa antibiotik baru. Tujuan dari penelitian ini adalah mengetahui aktivitas antibakteri dari lima isolat bakteri laut terhadap Staphylococcus aureus  dan mengidentifikasi jenis bakteri laut yang memiliki aktivitas antibakteri tertinggi dengan 16S rRNA. Hasil penelitian menunjukkan dari lima isolat bakteri laut, isolat M1.SP3121015.101.a menunjukkan aktivitas antibakteri tertinggi dengan rerata daya hambat sebesar 10,20 mm. Karakterisasi M1.SP3.121015.101.a dilakukan dengan menggunakan 16S rRNA didapatkan cluster gen pada ± 1500 bp. Berdasarkan sekuens isolat M1.SP3121015.101.a memiliki kemiripan dengan Bacillus tequilensis strain K2.4.2 dengan 100% kesamaan.  Penelitian ini memberikan manfaat yaitu didapatkannya  isolat bakteri laut yang memiliki kemampuan sebagai antibakteri dan dapat digunakan pada penelitian selanjutnya seperti proses isolasi senyawa bioaktif maupun proses optimasi.    Kata Kunci: 16S rRNA; Aktivitas Antibakteri; Bakteri Laut;  Staphylococcus aureus    ABSTRACT  A decreasing invention on new antibiotic and increasing disease caused by Staphylococcus aureus  has raised increasing interest in marine bacteria as promising producers of new antibiotic agent. The aim of this study is to explore marine bacteria activity to inhibit Staphylococcus aureus . The result showed that among five isolates of marine bacteria, the M1.SP3121015.101.a strain showed the highest antibacterial activity with inhibition zone was 10,20 mm. The characterization ofM1.SP3.121015.101.a was carried out using 16S rRNA, it showed that gene cluster at ±1500 bp. The sequences of M1.SP3121015.101.a  have been closes to Bacillus tequilensis strain K 2.4.2 with 100% similarity. This study provides some information about the isolate of marine bacteria that has the highest antibacterial activity and it can be used in further research such as the process of isolation of bioactive compounds and the optimization process.Keywords: 16S rRNA; Antibacterial Activity; Marine Bacteria; Staphylococcus aureus  
Dari Biji ke Cita Rasa: Pelatihan Brewing dan Cupping untuk Pemberdayaan Petani Kopi Desa Bayan Kabupaten Lombok Utara Cahayani, Mimi; Juniawan, Alvin; Swandayani, Rosalina Edy; Andini, Arista Suci; Fibrianto, Kiki; Rukmi, Widya Dwi; Pangestuti, Erdiana
Jurnal Pengabdian UNDIKMA Vol. 7 No. 1 (2026): February
Publisher : LPPM Universitas Pendidikan Mandalika (UNDIKMA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jpu.v7i1.18433

Abstract

This community service program aims to enhance the capacity of coffee farmer groups in post-harvest processing and professional coffee quality assessment, as well as to improve farmers’ understanding of coffee flavor profiles in order to increase their competitiveness in the market. The program was implemented through training and mentoring activities involving 35 coffee farmers from the “Muda Maju Tanjung Biru” group. Program evaluation was conducted using questionnaires and analyzed with a non-parametric statistical test (McNemar’s test). The results indicate that the training was effective in improving the partners’ knowledge and skills, as evidenced by a 79% increase in participants’ average knowledge scores after the program. In the cupping score training, 40% of participants (14 farmers) successfully achieved basic scoring competency. Overall, this community service program has proven to enhance farmers’ professionalism in coffee post-harvest processing and quality evaluation.
Co-Authors Agustin Mardiana, Nur Alvin Juniawan Andini, Arista Suci Joni Kusnadi KIKI FIBRIANTO Mimi Cahayani Pangestuti, Erdiana Rosalina Edy Swandayani Tutik Murniasih