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All Journal Jurnal Teknologi Pertanian Marine Research in Indonesia Makara Journal of Science Manilkara: Journal of Bioscience
Tutik Murniasih
Research Center for Oceanography Indonesian Institute of Sciences Jl. Pasir putih No. 1 Ancol Timur Jakarta 14430
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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CARBON SOURCE OPTIMIZATION FOR ANTIBIOTIC PRODUCTION FROM AAPTOS-ASSOCIATED BACTERIA Rhodobacteracea bacterium SP.2.11 Murniasih, Tutik; Bayu, Asep
Marine Research in Indonesia Vol 40, No 2 (2015)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.902 KB) | DOI: 10.14203/mri.v40i2.63

Abstract

Marine sponge Aaptos-aaptos is thought to produce antimicrobial aaptamine and its derivatives. To investigate whether its associated bacteria are in fact the producer of such bioactive compounds, a study of antibacterial compounds derived from Aaptos-associated bacteria was conducted. In this research, approximately 10 bacterial colonies were isolated from the sponge Aaptos aaptos. Among the bacteria isolated, the one that showed the most potential for producing antibacterial compounds was Rhodobacteracea bacterium. Extra and intracellular bacterial extract from this strain strongly inhibited the growth of pathogenic bacteria Staphylococcus aureus and Vibrio eltor, while were moderately effective against Bacillus subtilis. Optimization of antibacterial activity was conducted by culturing Rhodobacteraceae bacterium in various carbon sources such as glucose, lactose, amylum, molasses and glycerol. The highest production of biomass was obtained by culturing this bacteria in SYP (Seawater Yeast  Peptone) medium, enriched with 1% glycerol as the carbon source and with a harvesting time of around 56-104 hours. The highest activity (8 U/ml) was reached when culturing this strain in SYP medium without any adding of carbon sources. Data analysis using a statistical tool indicated that carbon sources added to medium do not have a significant effect on antibacterial activity. Characterizing the compound responsible for the antibacterial properties will be the topic of further work.Keywords : carbon source, Rhodobacteracea bacterium, Aaptos aaptos, associated bacteria 
BIODEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBON (PAH), PHENANTHRENE BY MARINE BACTERIUM THALASSOSPIRA SP. C.260 Murniasih, Tutik; Lisdiyanti, Puspita; Yopi, -
Marine Research in Indonesia Vol 35, No 1 (2010)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9518.239 KB) | DOI: 10.14203/mri.v35i1.4

Abstract

Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) compound that is known to be reported toxic to marine flora and fauna. Remediation of this environmental pollutant using chemical and physical methods causes environmental issues. Bioremediation using marine has been applied to degrade such various PAH compounds. Screening of marine microorganism in degrading this recalcitrant is very importance for bioremediation application in Indonesian waters. The purpose of this study was to screen and isolate bacterial with potential application in biodegradation of phenanthrene and other harmful PAH in marine environments. Several potential bacteria strains were isolated from oil contaminated sea water in Cilacap area. Sequence analysis using 16S rRNA gene marine bacterium strain C.260 showed 96% sequence homology to sequence of Thalassospira sp. In biodegradation of phenanthrene, within 28 days experiments, this bacterium degraded 50% and 99.75% of phenanthrene in medium with and without enrichment with NPK fertilizer respectively. Using sublimation method, this bacterium also degradeds phenothiazine, fluoranthene, and dibenzothiophene.
BIODIVERSITY OF HYDROCARBON-PRODUCING MICROALGAE FROM OIL CONTAMINATED IN COASTAL ZONE OF BATAM ISLAND Susilaningsih, Dwi; Siburian, Marlinang Diarta; Murniasih, Tutik
Marine Research in Indonesia Vol 33, No 2 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5544.715 KB) | DOI: 10.14203/mri.v33i2.484

Abstract

Addressing to the environmental and energy crises in Indonesia, the new alternative of green-economic-energy sources is required. In this study marine microalgae were isolated from oil-polluted coastal zone in Batam Island for alternative energy sources. The research was carried out by collecting the sea water samples from several peninsulas of Batam Island. The abundance of microalgal diversity was observed. The isolation was done by capillary micropipette technique. The method of specific screening for hydrocarbon-producing microalgae was done using selected media, CO2 rate and light intensity period treatments. The results showed that the Cyanobacteria and diatom occupied the Batams coastal waters. From the isolated samples, 11 strains positively deposit the hydrocarbon in their cells. Further study for determining the isolates and their properties is still in progress.
BIODEGRADATION OF POLYCYCLIC AROMATIC HYDROCARBON (PAH), PHENANTHRENE BY MARINE BACTERIUM THALASSOSPIRA SP. C.260 Murniasih, Tutik; Lisdiyanti, Puspita; Yopi, -
Marine Research in Indonesia Vol 35 No 1 (2010)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9518.239 KB) | DOI: 10.14203/mri.v35i1.4

Abstract

Phenanthrene is a polycyclic aromatic hydrocarbon (PAH) compound that is known to be reported toxic to marine flora and fauna. Remediation of this environmental pollutant using chemical and physical methods causes environmental issues. Bioremediation using marine has been applied to degrade such various PAH compounds. Screening of marine microorganism in degrading this recalcitrant is very importance for bioremediation application in Indonesian waters. The purpose of this study was to screen and isolate bacterial with potential application in biodegradation of phenanthrene and other harmful PAH in marine environments. Several potential bacteria strains were isolated from oil contaminated sea water in Cilacap area. Sequence analysis using 16S rRNA gene marine bacterium strain C.260 showed 96% sequence homology to sequence of Thalassospira sp. In biodegradation of phenanthrene, within 28 days experiments, this bacterium degraded 50% and 99.75% of phenanthrene in medium with and without enrichment with NPK fertilizer respectively. Using sublimation method, this bacterium also degradeds phenothiazine, fluoranthene, and dibenzothiophene.
CARBON SOURCE OPTIMIZATION FOR ANTIBIOTIC PRODUCTION FROM AAPTOS-ASSOCIATED BACTERIA Rhodobacteracea bacterium SP.2.11 Murniasih, Tutik; Bayu, Asep
Marine Research in Indonesia Vol 40 No 2 (2015)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (759.902 KB) | DOI: 10.14203/mri.v40i2.63

Abstract

Marine sponge Aaptos-aaptos is thought to produce antimicrobial aaptamine and its derivatives. To investigate whether its associated bacteria are in fact the producer of such bioactive compounds, a study of antibacterial compounds derived from Aaptos-associated bacteria was conducted. In this research, approximately 10 bacterial colonies were isolated from the sponge Aaptos aaptos. Among the bacteria isolated, the one that showed the most potential for producing antibacterial compounds was Rhodobacteracea bacterium. Extra and intracellular bacterial extract from this strain strongly inhibited the growth of pathogenic bacteria Staphylococcus aureus and Vibrio eltor, while were moderately effective against Bacillus subtilis. Optimization of antibacterial activity was conducted by culturing Rhodobacteraceae bacterium in various carbon sources such as glucose, lactose, amylum, molasses and glycerol. The highest production of biomass was obtained by culturing this bacteria in SYP (Seawater Yeast  Peptone) medium, enriched with 1% glycerol as the carbon source and with a harvesting time of around 56-104 hours. The highest activity (8 U/ml) was reached when culturing this strain in SYP medium without any adding of carbon sources. Data analysis using a statistical tool indicated that carbon sources added to medium do not have a significant effect on antibacterial activity. Characterizing the compound responsible for the antibacterial properties will be the topic of further work.Keywords : carbon source, Rhodobacteracea bacterium, Aaptos aaptos, associated bacteria 
BIODIVERSITY OF HYDROCARBON-PRODUCING MICROALGAE FROM OIL CONTAMINATED IN COASTAL ZONE OF BATAM ISLAND Susilaningsih, Dwi; Siburian, Marlinang Diarta; Murniasih, Tutik
Marine Research in Indonesia Vol 33 No 2 (2008)
Publisher : Research Center for Oceanography - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5544.715 KB) | DOI: 10.14203/mri.v33i2.484

Abstract

Addressing to the environmental and energy crises in Indonesia, the new alternative of green-economic-energy sources is required. In this study marine microalgae were isolated from oil-polluted coastal zone in Batam Island for alternative energy sources. The research was carried out by collecting the sea water samples from several peninsulas of Batam Island. The abundance of microalgal diversity was observed. The isolation was done by capillary micropipette technique. The method of specific screening for hydrocarbon-producing microalgae was done using selected media, CO2 rate and light intensity period treatments. The results showed that the Cyanobacteria and diatom occupied the Batam's coastal waters. From the isolated samples, 11 strains positively deposit the hydrocarbon in their cells. Further study for determining the isolates and their properties is still in progress.
POTENSI BAKTERI LAUT SEBAGAI SUMBER ANTIBIOTIK BARU PENGHAMBAT STAPHYLOCCUS AUREUS Mardiana, Nur Agustin; Murniasih, Tutik; Rukmi, Widya Dwi; Kusnadi, Joni
Jurnal Teknologi Pertanian Vol 21, No 1 (2020)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.714 KB) | DOI: 10.21776/ub.jtp.2020.021.01.6

Abstract

ABSTRAK Penemuan akan antibiotik baru yang semakin menurun dan meningkatnya penyakit yang disebabkan oleh Staphylococcus aureus  telah meningkatkan minat terhadap bakteri laut sebagai produsen senyawa antibiotik baru. Tujuan dari penelitian ini adalah mengetahui aktivitas antibakteri dari lima isolat bakteri laut terhadap Staphylococcus aureus  dan mengidentifikasi jenis bakteri laut yang memiliki aktivitas antibakteri tertinggi dengan 16S rRNA. Hasil penelitian menunjukkan dari lima isolat bakteri laut, isolat M1.SP3121015.101.a menunjukkan aktivitas antibakteri tertinggi dengan rerata daya hambat sebesar 10,20 mm. Karakterisasi M1.SP3.121015.101.a dilakukan dengan menggunakan 16S rRNA didapatkan cluster gen pada ± 1500 bp. Berdasarkan sekuens isolat M1.SP3121015.101.a memiliki kemiripan dengan Bacillus tequilensis strain K2.4.2 dengan 100% kesamaan.  Penelitian ini memberikan manfaat yaitu didapatkannya  isolat bakteri laut yang memiliki kemampuan sebagai antibakteri dan dapat digunakan pada penelitian selanjutnya seperti proses isolasi senyawa bioaktif maupun proses optimasi.    Kata Kunci: 16S rRNA; Aktivitas Antibakteri; Bakteri Laut;  Staphylococcus aureus    ABSTRACT  A decreasing invention on new antibiotic and increasing disease caused by Staphylococcus aureus  has raised increasing interest in marine bacteria as promising producers of new antibiotic agent. The aim of this study is to explore marine bacteria activity to inhibit Staphylococcus aureus . The result showed that among five isolates of marine bacteria, the M1.SP3121015.101.a strain showed the highest antibacterial activity with inhibition zone was 10,20 mm. The characterization ofM1.SP3.121015.101.a was carried out using 16S rRNA, it showed that gene cluster at ±1500 bp. The sequences of M1.SP3121015.101.a  have been closes to Bacillus tequilensis strain K 2.4.2 with 100% similarity. This study provides some information about the isolate of marine bacteria that has the highest antibacterial activity and it can be used in further research such as the process of isolation of bioactive compounds and the optimization process.Keywords: 16S rRNA; Antibacterial Activity; Marine Bacteria; Staphylococcus aureus  
BIODEGRADASI FENANTREN OLEH BAKTERI LAUT Pseudomonas sp KalP3b22 ASAL KUMAI KALIMANTAN TENGAH Murniasih, Tutik; Yopi, Yopi; Budiawan, Budiawan
Makara Journal of Science Vol. 13, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The Biodegradation of Phenanthren by Marine Bacteria Pseudomonas sp KalP3b22 from Kumai Central Borneo. The study of the potential marine bacteria that degrade Polycyclic Aromatic Hydrocarbons (PAHs) was already done among several marine bacterium isolated from the contaminated sea water collected in Kumai Port area. The biodegradation rate of phenanthrene by selected bacteria Pseudomonas sp Kalp-3b22 was 59,5% after 29 days cultivation. The structural determination of degradation product was deduced as 1-naphtalenol.
Uji Aktivitas Antimikroba dari Ekstrak Metanol Teripang Asal Perairan Lampung Untari, Febriana; Rasyid, Abdullah; Murniasih, Tutik; Wibowo, Joko Tri
MANILKARA: Journal of Bioscience Vol 3 No 2 (2025): Februari
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v3i2.11162.2025

Abstract

Sea cucumbers possess various biological activities, including antihypertensive, antioxidant, anticoagulant, antimicrobial, hemolytic, cytotoxic, and immunomodulatory properties. These activities are attributed to the presence of primary and secondary metabolites in sea cucumbers, such as peptides, vitamins, minerals, fatty acids, saponins, steroids, carotenoids, collagen, gelatin, chondroitin sulfate, and amino acids. This study aims to evaluate the antimicrobial activity of methanol extracts from several sea cucumber species collected from Lampung waters. The agar disk diffusion method was used in the study. The results of methanol extracts from five sea cucumber species tested againts five microbial species showed varying outcomes. The highest yield of sea cucumber methanol extract was found in Bohadschia marmorata (3.88%), while the lowest was Metriatyla lessoni (2.68%). In the antibacterial potential test, no antibacterial activity was observed against the four tested bacteria. However, the fungus Candida albicans exhibited an inhibition zone. Four sea cucumber species demonstrated antifungal potential against C. albicans, with the following inhibition zone diameters: B. Marmorata (13.35 ± 2.76 mm), Holothuria lessoni (12.9 ± 0.71 mm), Stichopus quadrifasciatus (10.4 ± 0 mm), and Holothuria leucospilota (8.25 ± 0.07 mm).
Co-Authors - Yopi Abdullah Rasyid Agustin Mardiana, Nur Bayu, Asep Bayu, Asep Budiawan Budiawan DWI SUSILANINGSIH Hani Mulyani, Hani Joko Tri Wibowo, Joko Tri Joni Kusnadi Maryani, Faiza Nina Artanti Pangestuti, Ratih PUSPITA LISDIYANTI Putra, Masteria Yunovilsa Rizna Triana Dewi Rukmi, Widya Dwi Saraswati, Vienna Siburian, Marlinang Diarta Siburian, Marlinang Diarta Untari, Febriana YOPI YOPI