Postoperative patients experience a metabolic stress response that may affect nitrogen balance and nutritional status, potentially leading to a prolonged length of hospital stay. This study aimed to analyze the relationship between nitrogen balance and nutritional status with the length of hospital stay among postoperative patients at Ulin Regional General Hospital, Banjarmasin. This study was an analytical observational study with a cohort prospektif design. The study sample consisted of 15 respondents selected using the total sampling technique. The data collected included respondent characteristics, nitrogen balance, nutritional status based on Mid-Upper Arm Circumference (MUAC), and length of hospital stay. The relationships between variables were analyzed using Spearman’s Rank correlation test. The results showed that 66.7% of respondents had a positive nitrogen balance, 53.3% had poor nutritional status before surgery, and 80% had a hospital stay of ≤7 days. The analysis indicated that nitrogen balance was not significantly associated with the length of hospital stay (p = 0.934; r = 0.023). Similarly, preoperative nutritional status (p = 0.936; r = 0.022) and postoperative nutritional status (p = 0.657; r = 0.125) were not significantly associated with the length of hospital stay among postoperative patients at Ulin Regional General Hospital, Banjarmasin. It is expected that hospitals, particularly healthcare professionals and nutrition departments, will enhance the monitoring of nutritional status and nitrogen balance while optimizing nutritional intake for postoperative patients.