<![CDATA[Cancer metastasis remains a major therapeutic challenge involving molecular dysregulation and tumor–microenvironment interactions. Stathmin 1 (STMN1), a microtubule-destabilizing regulator related to cell motility and tumor aggressiveness, is relevant for preliminary anti-metastasis-oriented screening. Nanostructured lipid carriers (NLC) may improve the dispersion and delivery performance of natural-product extracts. This study formulated shallot leaf (Allium cepa L.) extract into an NLC dispersion using emulsification–ultrasonication under homogenized and non-homogenized conditions, followed by PSA/DLS and SEM characterization. Phytochemical candidates curated from databases and literature were docked to STMN1 to identify ligands with favorable predicted binding profiles. Macrophage phagocytic function was evaluated using a latex-bead assay at 0, 5, and 10 µL. PSA/DLS and SEM indicated particulate structures, but particle-size variation and aggregation suggested the need for further formulation optimization and stability evaluation. Docking ranked sinapic acid (−7.5 kcal/mol) and ferulic acid (−7.4 kcal/mol) as the best phenolic candidates among the tested ligands, although the GDP control showed stronger predicted affinity. Phagocytic capacity increased from 33.5% (0 µL) to 37% (5 µL) and 91% (10 µL), while the phagocytic index increased from 0.63 to 0.77 and 4.50, respectively. The 10 µL dose was the most responsive condition for enhancing macrophage phagocytic activity. These findings should be interpreted as preliminary evidence of STMN1-oriented in silico prioritization and macrophage functional enhancement, not direct proof of anti-metastatic activity or M2 macrophage inhibition.]]>
