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Image Processing-Based Morris Water Maze Rat Tracking System: Design and Analysis Sutrisno Ibrahim; Gayatri Dewani; Joko Hariyono; Faisal Rahutomo; Nanang Wiyono; Ratih Yudhani
Journal of Fuzzy Systems and Control Vol. 4 No. 3 (2026): Vol. 4 No. 3 2026
Publisher : Peneliti Teknologi Teknik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59247/jfsc.v4i3.411

Abstract

Four experimental rats consisting of one normal rat and three Alzheimer's-induced rats were analyzed. The proposed system employed ImageJ integrated with the RatsTrack and Macro RatsTrack plugins using a centroid based tracking approach. The system successfully extracted movement trajectories, distance travelled, speed, acceleration, and quadrant dwell time during acquisition and probe trials. The system performance was validated by comparing quadrant dwell times obtained from the proposed system with manual observations. The percentage differences ranged from 2.29% to 70.97%. demonstrating that the proposed system can effectively represent rat behavioral patterns, although discrepancies remain in certain quadrants requiring further refinement.