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Pengaruh Ekstrak Etanol Pegagan (Centella asiatica ) Terhadap Volume Hippocampus Tikus (Rattus norvegicus) Pasca Stres Kronik Zulhamidah, Yenni; Wiyono, Nanang; Ratna Sari, Dwi Cahyani; Partadiredja, Ginus
Majalah Kesehatan Pharmamedika Vol 5, No 1 (2013): JUNI 2013
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/mkp.v5i1.1103

Abstract

Background. Chronic stress caused by restraint stress induces an increase in corticosterone (glucocorticoid) that result decreased hippocampal vollume. Centella asiatica has long been used for various neurological disturbances in Southeast Asian countries. Aims. The research aims to investigate the effects of ethanol extracts of Centella Asiatica to prevent the decrease of hippocampal vollume after chronic stress. Methods. Thirty male adult rats (Sprague Dawley) with body weight of 250-350 gram was randomly subdivided into six groups of treatments: Nonstress control group was given 2% Pulvis Gummi Arabicum (PGA), Stress control groups( PGA 2 %), groups treated with fluoxetine 10mg/kgbw/day , and Ca150,  Ca300, Ca600 containing ethanol extracts of Centella asiatica with doses 150 mg/kgbw/day, 300 mg/kgbw/day, 600 mg/kgbw/day a, respectively followed by restraint stress.  After 21 days of stress, rats underwent Morris Water Maze test for 6 days, perfused, and hippocampus was collected for histological processing. Toluidine blue staining is used to asses the estimation of hippocampal volume. Results. The estimated hippocampal volume were 3.1512 ± 1.01 (nonstress), 2.4736 ± 0.10 (stress), 2.7018 ± 1.06 (Ca150), 2.7405 ± 1.19 (Ca300), 2.2678 ± 0.82  (Ca600) and 2.5818 ± 0.70 (Fluoxetine). Statistic test showed that there were no significant diferrence between stress control group and groups treated. Conclusion. There is no the significant effects of Ethanol extracts of Centella Asiatica to prevent the decrease of hippocampal vollume after chronic stress.
Hubungan Tingkat Kecemasan dengan Tingkat Efikasi Diri Pada Atlet Renang Kusumadewi, Isnindri Annisa; Ghozali, Dhoni Akbar; Hastami, Yunia; Wiyono, Nanang
Journal of Sport Science and Education Vol 6, No 1 (2021)
Publisher : Fakultas Ilmu Olahraga, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jossae.v6n1.p107-114

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Anxiety might give the tremendous impact on swimming athletes such as loss of concentration and motor ability which could result in lowering athlete’s performance level in competition. Therefore, there was self efficacy as a psychological aspect which decreasing anxiety level on swimming athletes. A high self efficacy might enable athletes to face and handle the hard situations and also manage their anxiety in competition. This study aimed to determine whether there was a relationship between anxiety amd self efficacy in swimming athlete. This research was an observational study using a cross sectional approach. The subjects were 40 swimming athletes from Pyramid Swimming Club, Bogor aged 10-18 years old. The dependent variable was anxiety which measured using the Taylor Minnesota Anxiety Scale (TMAS) Questionnaire. The independent variable was self-efficacy which measured using a General Self-Efficacy (GSE) Questionnaire. Statistical analysis was performed using Product Moment Pearson test. The result showed there was correlation between anxiety and self-effcicay in swimming athletes as indicated with significance value of 0.031 and the correlation strength was low at 0.298 and the direction of correlation was negative. Based on these result it can be conclude that, there was negative correlation between anxiety and self-efficacy in swimming athletes. 
Modulasi Neurogenesis untuk Pengembangan Terapi Depresi -, Muthmainah; Wiyono, Nanang
Cermin Dunia Kedokteran Vol 44, No 12 (2017): Neurologi
Publisher : PT. Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.776 KB) | DOI: 10.55175/cdk.v44i12.694

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Depresi merupakan salah satu penyebab morbiditas dan mortalitas yang paling sering, diperkirakan 10-15% penduduk dunia pernah mengalami episode depresi selama hidupnya. Patofisiologi depresi kompleks dan melibatkan tingkat molekuler, seluler dan jaringan. Salah satu teori mekanisme depresi dan kerja antidepresan adalah proses neurogenesis. Neurogenesis pada masa dewasa di gyrus dentatus hippocampus dipengaruhi oleh stres yang berperan dalam patofisiologi depresi; proses neurogenesis ini dapat dimodulasi oleh antidepresan sehingga dapat dimanfaatkan untuk penatalaksanaan depresi. Antidepresan tersebut adalah Selective Serotonine Reuptake Inhibitors (SSRIs) dan Norepinephrine Reuptake Inhibitors (NRIs).Depression is one of the most common causes of mortality and morbidity; it is estimated that 10-15% of world population ever experienced an episode of depression. The pathophysiology of depression is very complex involving mechanism in the molecular and cellular level. The neurogenesis hypothesis postulates that decreased neurogenesis results in depressive phenotype and that neurogenesis is crucial for the behavioural effect of antidepressant. Decreased adult neurogenesis in the dentate gyrus of the hippocampus is induced by stress. Modulation of neurogenesis in this area through administration of antidepressant such as SSRIs and NRIs has been used for the treatment of depression.
THE EFFECT OF ETHANOLIC EXTRACT OF CENTELLA ASIATICA (L) URBAN ADMINISTRATION TOWARDS THE THICKNESS OF PYRAMIDAL LAYER IN THE CA1 REGION OF HIPPOCAMPUS OF SPRAGUE DAWLEY RATS AFTER CHRONIC RESTRAIN STRESS Anggraeni Janar Wulan; Brian Wasita; Nanang Wiyono
JUKE Unila Vol 4, No 8 (2014)
Publisher : Fakultas Kedokteran Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.527 KB)

Abstract

Background: Stress has been shown can change the structure of the brain, especially in the cornu ammonic (CA) I region of hippocampus. Centella asiatica or pegagan as herbal neuroprotector was investigated. The study aimed to determine the effect of ethanolic extract of C. asiatica to the thickness of pyramidal layer in the CA1 region in rats subjected to chronic restrain stress (CRS). Method: This experimental study used post test only group design. Thirty five rats were divided into 6 groups: non stress group (control) and stress only group pulvis gum arabicum (PGA), positive control group (treated with fluoxetin 10mg/kgBW), and 3 treatment groups are treated with C. asiatica 150, 300, and 600mg/kgBW respectively. All groups except non stress group were subjected to CRS, for 6 hours per day for 21 days. Fluoxetin, PGA, and C. Asiatica were applied 30 minutes before CRS done. Rats were sacrificed at the end of 24th day and were perfused transcardially. Processed brain tissue stained with toluidine blue, and measured with Image Raster program. Result: The thickness of pyramidal layer in the CA1 region are increasing significantly in treatment groups which treated with 150 and 300 kg/BW of C. asiatica, but not significant which treated with 600mg/kgBW ethanolic extract of C. asiatica. Conclusion: Ethanolic extract of C. asiatica can improve thickness of pyramidal layer in the CA1 region of hippocampus of Sprague Dawley rats after chronic restrain stress. [JuKe Unila 2014; 4(8):202-207]
PENGARUH MENDENGARKAN MUROTTAL AL-QUR’AN TERHADAP MEMORI KERJA Istiqomah Nur Aziza; Nanang Wiyono; Afia Fitriani
Psikis : Jurnal Psikologi Islami Vol 5 No 1 (2019): Psikis: Jurnal Psikologi Islami
Publisher : Program Studi Psikologi Islam, Fakultas Psikologi, Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Psikis.v5i1.2547

Abstract

It is important to optimize working memory because it transforms, synergizes and constantly updates new and old information. One way to optimize working memory is to listen to Murottal Al-Qur'an, because it has a harmonious tone which can stabilize the mind to properly process the information. This study aims to determine the effect of listening to the Al-Quran murottal on working memory. The research subjects were 24 students of Psikologi 2017, grouped equally in the control and experimental groups. An experimental intervention was administered for 15 consecutive days lasting 15 minutes and 52 seconds. Measurements in working memory use Operation Span Task, Reading Span Task and Symmetry Span Task. The design of the study used a pre-test post-test control group and the data were analyzed by t-test. The results showed a significant difference between the control group and the experimental group on the symmetry span task subtest (p = 0.044, p <0.05).
POTENTIAL USE OF VIRTUAL REALITY IN OVERCOMING NEEDLE PHOBIA IN COVID-19 VACCINATION Penggalih Mahardika Herlambang; Nanang Wiyono; Hendry Gunawan; Rani Tiyas Budiyanti
JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL) Vol 10, No 6 (2021): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v10i6.31504

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Background: Vaccination is very important because it can help prevent diseases that can be prevented by immunization (PD3I) such as polio, tetanus, diphtheria, measles, mumps, and rubella. During the Coronavirus Disease -19 (COVID-19) pandemic, the role of vaccination is very crucial, especially to reach herd immunity. However, there are many problems related to the implementation of vaccination, including needle phobia, which is a phobia of needles because it is considered painful. Virtual reality is a technology that develops in the form of a virtual image that can be used in vaccination services. This study aims to determine the potency of virtual reality to solve the needle phobia in vaccination services.Methods: The method used in this study is a literature review with sources from journals in the Scopus, ProQuest, and ScienceDirect database that was published in 2000 until 2020, and also books and articles with the keywords "virtual reality" "AND" "immunization," virtual reality " AND " vaccination " , "Needle phobia" "AND" "vaccination". This research was conducted from January to February 2021.Results: Based on the results obtained, virtual reality can be used in vaccination services, especially in the treatment of needle phobia. Virtual reality can become a distraction so that the patient will be distracted from fear and can lower the pain threshold. In addition, virtual reality can also be used as a means of education about immunization.Conclusion: Product development and clinical research need to be developed to assess the use of virtual reality in vaccination services during the COVID-19 pandemic. The use of virtual reality for other medical procedures that use needles can also be further investigated
The Effects of Reading Activity on Episodic Memory in Elderly Ummu Ma’rifatul Afifah; Nanang Wiyono; Yunia Hastami; Siti Munawaroh
Berkala Kedokteran Vol 17, No 1 (2021)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (247.154 KB) | DOI: 10.20527/jbk.v17i1.10269

Abstract

Abstract: The aging process is a natural event that cannot be avoided. Especially in the elderly, this is accompanied by a decrease in episodic memory function, which can reduce the quality of life. Therefore, efforts are needed to maintain cognitive function in elderly, one of which is through routine cognitive activities such as reading which can affect memory function in elderly through neuroplasticity. This study aims to determine the effect of reading activity on episodic memory function in the elderly. This research is a quasi-experimental study with a pre-test post-test control group design method. The total research subject were 30 people in Desa Demangan Siman, Kabupaten Ponorogo and Kelurahan Ngadirejo, Kota Kediri, with a sample technique using random purposive sampling. The research subjects were then divided into 3 groups with random allocation techniques using microsoft excel. Group 1 was the control group, group 2 was the 15-minute reading aloud intervention group, and group 3 was the 30-minute reading aloud intervention group. The results of the Dependent T-test showed that there was a significant difference in mean scores between the pre-test and post-test scores in group 2 (p = 0.000) and group 3 (p = 0.000), but not significant in group 1 (p = 0.111). The One Way ANOVA test results showed a significant difference in post-test scores in the three groups (p = 0.043), and the Post-hoc LSD test found a significant difference in scores between the post-test scores in group 1 with group 2 (p = 0.025) and group 1 with group 3 (p = 0.034), but there was no significant difference between the post-test scores in group 2 with group 3 (p = 0.883). It can be concluded, there is a significant effect between the duration of reading activity and episodic memory function in the elderly. Keywords: aging, elderly, episodic memory, reading, neuroplasticity.
Centella asiatica increases B-cell lymphoma 2 expression in rat prefrontal cortex Kuswati Kuswati; Djoko Prakosa; Brian Wasita; Nanang Wiyono
Universa Medicina Vol. 34 No. 1 (2015)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2015.v34.10-16

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BACKGROUNDStress is one of the factors that cause apoptosis in neuronal cells. Centellaasiatica has a neuroprotective effect that can inhibit apoptosis. This studyaimed to examine the effect of Centella asiatica ethanol extract on B-celllymphoma 2 (Bcl-2) protein expression in the prefrontal cortex of rats.METHODSAn experimental study was conducted on 34 brain tissue samples from maleSprague Dawley rats exposed to chronic restraint stress for 21 days. Thesamples were taken from following groups: non-stress group K, negativecontrol group P1 (stress + arabic gum powder), P2 (stress + C.asiatica at150 mg/kgBW), P3 (stress + C.asiatica at 300 mg/kg BW), P4 (stress +C.asiatica at 600 mg/kg body weight) and positive control group P5 (stress+ fluoxetine at 10 mg/kgBW). The samples were made into sections thatwere stained immunohistochemically using Bcl-2 antibody to determine thepercentage of cells expressing Bcl-2. Data were analyzed using one wayANOVA test followed by a post - hoc test.RESULTSThere were significant differences in mean Bcl-2 expression between thegroups receiving Centella asiatica compared with the non-stress group andstress-only group (negative control group) (p<0.05). The results werecomparable to those of the fluoxetine treatment group.CONCLUSIONThe Centella asiatica ethanol extract was able to increase Bcl-2 expressionin the prefrontal cortex of Sprague Dawley rats exposed to restraint stress.This study suggests that Centella asiatica may be useful in the treatment ofcerebral stress.
DELPHI TECHNIQUE: CONSENSUS OF ANATOMY CIRCULATORY SYSTEM CORE SYLLABUS FOR MEDICAL STUDENT Siti Munawaroh; Farhah Millata Hanifa; Nanang Wiyono; Yunia Hastami; Nur Dewi Kartikasari; Bulan Kakanita Hermasari
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 7, No 2 (2018): JULI
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (254.034 KB) | DOI: 10.22146/jpki.39101

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Background: Anatomy is a very important basic science in medical education. However, the advance of science and technology affected medical curriculum. One of the changes is the decreased time available for anatomy learning. Therefore, an anatomy lecturer needs to sort and choose from many existing anatomical materials to adjust with time allocation. The purpose of this study is to gain consensus on what minimum anatomical knowledge is for a physician candidate in order to practice safely. Method: This study was design in qualitative approach with two round Delphi Technique. Using purposive sampling method, the subjects of the study were 20 general-physicians in Indonesia. The questionnare was arranged based on anatomy text book and the research about anatomic core syllabus. The firts Delphi aimed to select the anatomical course material and additional course material. The second Delphi round intended to provide level range from 1-4. The consensus level established by researcher at 60% point.Results: There is not any additional topics in the first Delphi round. So the number of first and second round topics is 270 topics. The Delphi second round agree that anatomic core syllabus of circulation system is 110 of 270 topics. There are 30 topics of cor, 3 topics of pericardium, 10 topics of mediastinum, 37 topics of artery, 9 topics of vena, 9 topics of lymphathic system, 4 topics of postnatal circulation, 5 topics of prenatal circulation, and 3 topics of portal hepatic system.Conclusion: The anatomy core syllabus consists of 110 of the 270 anatomical circulatory system topics.
LEARNING OBJECTIVE OF THE ANATOMY CIRCULATION SYSTEM FOR THE MEDICAL STUDENTS: A DELPHI STUDY Marwatunnisa Al Mubarokah; Siti Munawaroh; Yunia Hastami; Nanang Wiyono
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 9, No 3 (2020): November
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpki.48768

Abstract

Background: Anatomy is basic science that is important for preclinical students, clinics, and specialists. The development of medical science led to a reduced allocation of learning time in Anatomy. The imbalance between the amount of learning material with time allocation makes various effective teaching methods carried out and examined. The learning objectives guide the faculty in selecting teaching methods as well as appropriate evaluation to measure the progress of student understanding. This study aims to obtain detailed anatomical learning objectives regarding the circulation system for medical students as part of the development of the medical curriculum in Indonesia.Method: This research is qualitative research with the modified Delphi method. The research subjects were anatomy lecturers in Indonesia who. The first round of the Delphi questionnaire was filled out by selecting the Bloom cognition domain between C1-C6 and the respondent composing the sentence of learning objectives according to the core material. The second round of respondents was asked to choose an important level from each point of learning objectives on the Likert Scale 1-4. The consensus level chosen is that 60% of the panel chooses on level 3 or 4.The results of the second round are then processed to ensure the most appropriate selection of operational verbs and cognitive domains.Results: The first Delphi round resulted in 74 learning objectives from 110 core material points with the cognitive domain between C1-C3. While the second round, Delphi generates 59 learning objectives. The third round which was not part of the Delphi method, these results were then consulted to anatomists of the circulatory system and came up with the 32 learning objectives.Conclusion: The learning objectives of the circulation system that need to be known are 32 learning objectives, with cognitive domains varying between C1-C3, include cor, pericardium, mediastinum, arteries, veins, lymphatics system, prenatal and postnatal circulation, dan hepatic portal  system. Keywords: anatomy of circulatory system, Delphi method, anatomy learning objectives, lecturer consensus 
Co-Authors -, Muthmainah Abdullah Faqih Abdurrahman Afa Haridhi Achmad Nurul Hidayat Adam Fauzi Adhi, Auliya Yasmin Afia Fitriani Agus Supinganto Agus Supriyanto Amaliah, Lili Amru, Khaeriah Ana Tsalitsal Futiya Roisal Ufqi Anggraeni Janar Wulan Annang Giri Moelyo Anugrah, Tessa Septian Ari Handono Ramelan Ari Probandari Aripa, Lusyana Aris Sudiyanto Aryoseto, Lukman Baharuddin Baharuddin Bakti, Alfonsus Aryo Sunu Baskoro, Reza Setyo Brian - Wasita,dr.,Ph.D Brian Wasita Brian Wasita Brian Wasita Brian Wasita Budi Purnama Budiono, Enrico Ananda Budiyanti Wiboworini Bukhari, Agussalim Bulan Kakanita Hermasari Bulan Kakanita Hermasari Devinta, Salzha Dhoni Akbar Ghozali Dhoni Akbar Ghozali Diah Kurnia Mirawati Dian Fikri Rachmawan Diana Nurrohima Dicky Maulana Lazuardi Djoko Prakosa Dorce Sisfiani Sarimin Duwi Handayani, Juleha Dwi Cahyani Ratna Sari Dwi Teguh Raharjo Dyonisa Nasirochmi Pakha Endang Sri Wahyuni Evy Aryanti Fairuz Zamani Faisal Rahutomo Farhah Millata Hanifa Farida Hidayati Ferdian, Hanum Ferianasari, Ineke Winda Firnadi, Jossefa Gabriela Claudia Ginus Partadiredja Hadyan, Rifqi Handayani, Damiana Nirmala Mukti Handayani, Selfi Handayani, Selfi Hanifa, Shafira N. Haridhi, Abdurrahman Afa Hastami, Yunia Hendry Gunawan Herawati, Fitriana Hidayat, Achmad Nurul Hilmy, Farhan Ida Nurwati Ida Nurwati Ilyas, Muhana F. Ilyas, Muhana Fawwazy Ina Kusrini INTAN PRATIWI Irawan, Amelia Tjandra Isna Qadrijati Istiqomah Nur Aziza Joko Hariyono Jossefa Almanita Haepie Firnadi Jusup, Sinu Andhi Kusumadewi, Isnindri Annisa Kusumaningrum, Sulistyani Kuswati Kuswati Lazuardi, Dicky Maulana Lukas, Graciella A. Lutfatul Jannah, Safella M. Nur Dewi Kartikasari Marwatunnisa Al Mubarokah maryatun maryatun Mashudi, Sugeng Mashuri, Yusuf Ari Mohtar Yunianto Muchtar Hanafi Muhammad Fanani Muhammad Farrel Akshya Muhammad Syaifullah Muthaminah, Muthaminah Muthmainah Muthmainah - Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah Muthmainah, Muthmainah Nandyantami, Farah Fauziah Nugroho, Dian Nunqaf, Anjani Maulaya Nurdin, Nugi Nurhadi, Asty A. Nurhani, Ahmad Isnaini Shidqi Nurul Mahmudah, Irvina Nurwijayanti Nuryani Nuryani Nuryani Nuryani Palupi, Pratiwi Indah Paramasari Dirgahayu Penggalih Mahardika Herlambang Pesik, Riza N. Prasetya Utami Pratiaksa, Ardian Pratiwi Indah Palupi Probandari, Ari Rachmi Fauziah Rahayu Raditya Perdana Aryasatya Rahayu, Rachmi F. Rahma, Annisa A. Rahma, Annisa Aghnia Rahmadany, Muhammad Fa'iz Rahmadany, Muhammad Faiz Rahmani, Muhammad Z. Rahmat Hidayat Rahmat Rohmani Rahmatillah, Arif Rani Tiyas Budiyanti Ratih Dewi Yudhani Ratih Puspita Febrinasari Ratih Yudhani Retno Hartati Ridhallah, Muhammad Syauqi Riyanto, Agus Sugeng Riyatun Riyatun Riza Novierta Pesik Rohmaningtyas Hidayah Setyaningrum Sayekti Wahyuningsih Selfi Handayani Selfi Handayani Selfi Handayani Selfi Handayani Selfi Handayani Sentot Budi Rahardjo Shabrina, Syania Shariza Fakurazi Sholikah, Tri Agusti Sinu Andhi Jusup Siswanto, Febby Gunawan Siti Munawaroh Siti Munawaroh Siti Munawaroh Suharno Suharno Sutrisno Ibrahim Suyatmi Suyatmi Tamaulina Br Sembiring Tandiyo, Desy Kurniawati Tedja Danudjo Oepomo Tri Rejeki Andayani Triniputri, Winastari Y. Ummu Ma’rifatul Afifah Utari Utari Utomo, Hernowo Setyo Veronika Ika Budiastuti Wardhani, Lusi O. Wardhani, Lusi Oka Wasita, Brian Wasita,dr.,Ph.D, Brian - Wijayanto, Matthew A. Windy Ayu Lestari Yarsa, Kristanto Y. Yarso, Kristanto Yuli Yenni Zulhamidah, Yenni Yudhani, Ratih D. Yunia Hastami Yuridian Purwoko, Reza Yusuf Anggara Aji Yuyun Yueniwati Zahrah, Fathimah Afifah Zaki, Shifa Nurzahra