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Nurhadiyanta
Department of Biology, Indonesia Defense University, Indonesia Peace and Security Center (IPSC) Sentul, Bogor 16810, Indonesia

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Microbial biofertilizer-induced fatty acid and squalene modulation for enhancing plant resilience under CBRNE-related abiotic stress Aditya; Mentari Putri Pratami; Minandre Wiratama; Nurhadiyanta
CBRNE Defense Studies Vol. 1 No. 1 (2026): CBRNE Defense Studies, Vol. 1 No. 1, June 2026
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55749/cds.v1i1.166

Abstract

Abiotic stress is one of the main factors that cause a decline in plant growth and productivity. The use of microorganism-based biofertilizers can be an environmentally friendly alternative to enhance plant resistance to extreme environmental conditions. This study aims to analyze the role of microorganisms in biofertilizers on the profile of fatty acids and squalene related to plant abiotic stress resistance. Metabolite analysis was carried out using a metabolomic approach and metabolic pathway mapping through the KEGG database. Identification results showed the presence of important compounds such as palmitic acid, oleic acid, stearic acid, phytol, squalene, alpha-tocopherol, stigmasterol, and sitosterol. These compounds play a role in cell membrane stability, antioxidant activity, and protection against oxidative stress. Pathway analysis shows the involvement of the Glycosylphosphatidylinositol (GPI)-anchor biosynthesis pathway, which is related to membrane metabolism and the cellular response to environmental stress. The research results indicate that microorganisms in biofertilizers have the potential to enhance plant adaptation to abiotic stress through the regulation of lipid metabolism and the production of bioactive compounds. Thus, microorganism-based biofertilizers can be developed as an environmentally friendly alternative to support sustainable agriculture.
Characterization of bioactive compounds in NPK biofertilizer as a sustainable plant growth promoter to mitigate CBRN environmental and chemical risks Muhammad Arya Nurzabal; Mentari Putri Pratami Pratami; Nurhadiyanta
CBRNE Defense Studies Vol. 1 No. 1 (2026): CBRNE Defense Studies, Vol. 1 No. 1, June 2026
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55749/cds.v1i1.167

Abstract

Metabolite analysis of NPK fertilizers based on biofertilizers was performed using Gas Chromatography-Mass Spectrometry (GC-MS) technique to identify the presence of bioactive compounds inside fertilizers. The identification of the metabolites revealed the presence of various metabolite compounds such as fatty acids, esters, alcohols, and sterol derivatives. Identified compounds were mapped using MetaboAnlayst and showed the association with steroid biosynthesis pathway. This pathway is associated with plant sterol, ergosterol, and brassinosteroid biosynthesis, which play roles in plant growth and soil microorganisms' activity. The existence of compounds like oleic acid and sterol derivatives indicates that NPK fertilizer can be utilized not only as a source of macronutrients but also as a biofertilizer that enhances soil biological activities and plant growth.