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All Journal SITAWA : Jurnal Farmasi Sains dan Obat Tradisional
Khiky Dwinatrana
Program Studi Farmasi Klinis, Fakultas Ilmu Kesehatan, Universitas Baiturrahmah, Padang, Sumatera Barat

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Antibacterial Potential Of Endophytic Fungi Isolated From Pachyrhizus Erosus (Bengkoang) Khiky Dwinatrana; Fadilla Lira; Rustini Rustini; Ferdian Arif
SITAWA : Jurnal Farmasi Sains dan Obat Tradisional Vol. 5 No. 1 (2026): SITAWA : Jurnal Farmasi Sains dan Obat Tradisional
Publisher : LPPM Akademi Farmasi Imam Bonjol Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62018/sitawa.v5i1.190

Abstract

The search for new antibacterial agents has increasingly focused on endophytic fungi, which inhabit plant tissues and may complement the bioactivity of their hosts. Pachyrhizus erosus (bengkoang), an edible legume rich in phenolics and flavonoids, remains underexplored as a reservoir of fungal endophytes with antibacterial potential. This study aimed to isolate endophytic fungi from P. erosus and evaluate their antibacterial properties. Tuber and leaf tissues were surface sterilized, yielding five isolates (PE1–PE5) that were cultured on rice medium, extracted with ethyl acetate, and tested against Staphylococcus aureus ATCC 29213 and Escherichia coli ATCC 25922 using the Kirby–Bauer disc diffusion method. All extracts inhibited bacterial growth, with PE2 showing the strongest activity (15.7 ± 1.40 mm for S. aureus and 14.4 ± 0.32 mm for E. coli). TLC profiling of PE2 extract revealed multiple spots under UV (254 and 366 nm), and phytochemical screening indicated the presence of phenolics and triterpenoids. Taken together, the results demonstrate that tuber-associated endophytic fungi of P. erosus are capable of producing antibacterial metabolites comparable to plant-derived compounds, underscoring their potential as sources of antimicrobial agents.
Antibacterial, antioxidant, and cytotoxic potential of an endophytic Fusarium verticillioides from yacon (Smallanthus sonchifolius) leaves Khiky Dwinatrana; Nofrizal; Arif Ferdian; Frisca Rachmadhany; Reka Dwi Aprodita; Putri Aprilia; Dina Dwi Febrina
SITAWA : Jurnal Farmasi Sains dan Obat Tradisional Vol. 5 No. 2 (2026): SITAWA : Jurnal Farmasi Sains dan Obat Tradisional
Publisher : LPPM Akademi Farmasi Imam Bonjol Bukittinggi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62018/sitawa.v5i2.275

Abstract

Endophytic fungi, residing within plant tissues without causing disease, serve as an important reservoir of bioactive compounds with pharmaceutical potential. This research focuses on isolating and identifying endophytic fungi from the leaves of Smallanthus sonchifolius and evaluating their antioxidant, antibacterial, and cytotoxic activities. A total of five fungal isolates (DY1–DY5) were obtained and subjected to in vitro bioassays and molecular identification. Internal transcribed spacer (ITS) sequencing revealed that isolate DY5 corresponded to Fusarium verticillioides, which consistently demonstrated notable bioactivities. Antioxidant activity was confirmed through the DPPH radical scavenging assay, where DY5 exhibited an IC₅₀ value of 241.23 μg/mL. Antibacterial evaluation against Escherichia coli ATCC 25922 revealed an inhibition zone of 12.22 ± 0.98 mm and a minimum inhibitory concentration (MIC) of 500 μg/mL. Cytotoxic screening using the Artemia salina lethality assay indicated moderate toxicity, with LC₅₀ values of 197.54 μg/mL for DY1 and 386.32 μg/mL for DY5, thereby meeting the threshold of bioactive extracts. This study provides evidence that endophytic fungi from S. sonchifolius, notably F. verticillioides (DY5), can serve as potential sources of bioactive compounds with pharmaceutical relevance. Further research focusing on metabolite profiling, compound isolation, and mechanistic evaluation is warranted to elucidate their chemical diversity and therapeutic significance in drug discovery efforts
Co-Authors Arif Ferdian Dina Dwi Febrina Fadilla Lira Ferdian Arif Frisca Rachmadhany Nofrizal Putri Aprilia Reka Dwi Aprodita Rustini Rustini