Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal Bioscientist : Jurnal Ilmiah Biologi
Herlina Ekapratama Dewi
Universitas Nahdlatul Ulama Kalimantan Timur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
DNA Barcoding for Species Identification and Antioxidant Evaluation of Kapul (Baccaurea spp.): Implication for The Conservation of Native Plants of Kalimantan Herlina Ekapratama Dewi; Umul Karimah; Putri Rinda Pratiwi; Rizky Amelia; Kinasih Prayuni
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 3 (2026): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i3.20411

Abstract

This study aimed to characterize the molecular identity of three morphologically distinct Kapul types from West Kutai, East Kalimantan, using DNA barcoding based on the matK and rbcL genes, and to evaluate the antioxidant activity of their leaf extracts. Leaf samples were subjected to DNA extraction, PCR amplification, Sanger sequencing, and subsequent BLAST comparison against reference sequences available in the GenBank database. The BLAST analysis revealed high sequence similarity with several Baccaurea species, with sequence identity values generally exceeding 98%. However, the highest similarity matches were not consistently assigned to a single species across different barcode regions, indicating limitations of the matK and rbcL markers in resolving species-level identification among closely related taxa. Antioxidant evaluation of ethanolic leaf extracts demonstrated strong activity, with IC₅₀ values of 6.94 ppm, 10.30 ppm, and 15.08 ppm for Samples 1, 2, and 3, respectively. These findings reveal high molecular similarity among the investigated Kapul types and provide preliminary evidence of their antioxidant potential, while highlighting the need for additional molecular markers to improve species-level identification and support future conservation studies.
Co-Authors Kinasih Prayuni Putri Rinda Pratiwi Rizky Amelia Umul Karimah