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All Journal Bioscientist : Jurnal Ilmiah Biologi Journal of Sustainable Transformation Jurnal Sains dan Kesehatan
Umul Karimah
Universitas Nahdlatul Ulama Kalimantan Timur
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences

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Pengadaan Awal Fasilitas Pemeliharaan dan Upaya Perolehan Filial 1 (F1) Ikan Zebra (Danio rerio) sebagai Hewan Laboratorium Umul Karimah
Bioscientist : Jurnal Ilmiah Biologi Vol 9, No 1 (2021): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v9i1.3750

Abstract

Zebra fish (Danio rerio) is a laboratory animal that can be used for a wide field of research, testing, and education. Laboratories that wish to use Zebra Fish must have their own Zebra Fish rearing facility. The next step is to breed parental Zebra Fish from external sources until filial 1 (F1) is obtained in the facility which is then used later. This research is descriptive experimental, which describes the practical experience of providing maintenance, breeding, and rearing facilities for embryos and larvae. Quarantine specific facilities, number of tanks, water pH conditions, breeding methods, facility cleaning routines, and special larval feeds need to be considered for the success of early stage rearing facilities. This study showed that the embryo safety rate ranged from 38.2% to 88.9%. Meanwhile, for larvae up to the age of 21 dpf it ranged from 1.5%-9.1%, which indicated that the rearing conditions were still suboptimal, especially larval rearing. Facility procurement and maintenance costs are divided into mandatory and optional costs depending on the needs, location and time of the maintenance facility. Laboratories can independently design maintenance facilities that are effective to meet research needs and are cost efficient.
DETEKSI DAN KUANTIFIKASI RHODAMIN B PADA PRODUK LIPSTIK TANPA NOMOR NOTIFIKASI BPOM YANG BEREDAR DI PASAR MALAM KECAMATAN PALARAN SAMARINDA juliyanti juliyanti; Umul Karimah; Lina Apriyani
Journal of Sustainable Transformation Vol. 1 No. 1 (2022)
Publisher : Universitas Nahdlatul Ulama Kalimantan Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59310/jst.v1i1.6

Abstract

Rhodamine B is one of chemical compound that is the most widely used in lipstick sold in have no a notification number from the drug and food regulatory agency, with physical characteristics that have a very light color and then when applied to the skin it is difficult to remove the traces. This research purposed to determine the presence of Rhodamine B in lipstick from the characteristics of lipstick containing Rhodamine B and to know the concentration of Rhodamine B in the sample. The method used in this study is qualitative and quantitative, with the selection of samples by purposive sampling. Qualitative identification in this study using a Rhodamine B test kit, meanwhile quantitative identification using UV-Vis Spectrophotometry. The results of the qualitative test using the Rhodamine B Test Kit contained 4 positive samples, sample 3,4,5 and 7. Rhodamine B concentration in positive samples were 34.76 ppm (sample 3), 38.30 ppm (sample 4), 74.28 ppm (sample 5), and 108.38 ppm (sample 7). Based on the qualitative analysis, the number concentration of Rhodamine B in the sample did not affect the color of the lipstick product, thus these samples were difficult to identify sample containing Rhodamine B. The results of this study indicated that there are still cosmetic manufacturers using the Rhodamine B as dye in lipstick production. Moreover, these positive samples do not meet the criteria for safe cosmetic products in terms of packaging, incomplete labels, heterogenous texture. Keywords: Lipstick, Rhodamine B, Spectrophotometry Uv-Vis, Test kit
Migration Behavior of Immunoglobulin Y in Native Page Electrophoresis Umul Karimah
Jurnal Sains dan Kesehatan Vol. 2 No. 1 (2019): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v2i1.104

Abstract

ABSTRACT Protein separation with electrophoresis in native state (native Polyacrylamide Gel Electrophoresis, native PAGE) is rarely applied in laboratory which results in limited scientific report. Native PAGE result can be used in further assay analysis or shows important protein-protein interaction. This study aimed to investigate immunoglobulin Y(IgY) behavior in separation using native PAGE. IgY protein is used since many properties of IgY are already known. IgY was isolated from follicles yolk and examined using clear native PAGE (CN-PAGE) and Sodium Dodecyl Sulphate (SDS) PAGE, both in reducing and nonreducing condition. CN-PAGE electropherogram of IgY showed streaked band from ~409-1048 kDa indicating IgY aggregation. Whereas SDS-PAGE in reducing condition showed normal result, ~68 kDa and ~18,7 kDa protein bands correlated with heavy and light chain of IgY respectively. Moreover SDS PAGE in nonreducing condition also resulted in normal IgY band sized ~185 kDa. Dilution and disaggregation using sodium dodecyl sulphate (SDS), sodium deoxycholate (SDC), sarcosyl, urea, and glycerol did not affect the migration behavior. We concluded that IgY is not in aggregate form. Migration behavior of IgY in CN-PAGE which was slow, produced streaked band, and appeared to have very high molecular weight is possibly due to low electronegativity (pI = 6,6±0,9) in CN-PAGE condition (buffer pH 8,3) and planar conformation of IgY. Keywords: electrophoresis, immunoglobulin Y, native PAGE.
Co-Authors juliyanti juliyanti Lina Apriyani