Introduction: Pregnancy and childbirth are natural physiological processes rather than pathological conditions. However, normal pregnancy and labor may develop into complications under certain circumstances. Although childbirth is expected to be a joyful experience, many pregnant women experience fear, anxiety, and emotional distress while anticipating labor. Maternal anxiety during pregnancy may stimulate uterine contractions through neuroendocrine responses, potentially affecting the labor process. Complementary non-pharmacological interventions, such as hypnobirthing relaxation and lavender aromatherapy, have been widely used to promote relaxation and reduce anxiety during pregnancy. This study aimed to determine the effect of hypnobirthing relaxation combined with lavender aromatherapy on anxiety levels among third-trimester pregnant women in preparation for childbirth at PMB Putu Ana. Method: This study employed a quantitative quasi-experimental design using a non-equivalent control group approach. A total of 56 third-trimester pregnant women were recruited through purposive sampling and allocated into two groups: an intervention group (n = 28), which received hypnobirthing relaxation combined with lavender aromatherapy, and a control group (n = 28), which received routine antenatal care without the intervention. Data were analyzed using the Mann–Whitney U test. Result: The Mann-Whitney test yielded p = 0.000 (p < 0.05), indicating that H0 was rejected. Therefore, it can be concluded that hypnobirthing relaxation combined with lavender aromatherapy affects the anxiety levels of third-trimester pregnant women preparing for childbirth at PMB Putu Ana. Conclusion: There is an Effect of Hypnobirthing Relaxation and Lavender Aromatherapy on the Anxiety Level of Pregnant Women in the Third Trimester Facing Childbirth Preparation at PMB Putu Ana.