Interactive multimedia is a technology-based learning medium that integrates text, video, audio, and animation with interactive elements to create a more engaging and less monotonous learning experience for students. It serves as an alternative technology-based learning medium to support students at SMAN 1 Srengat in learning about Basidiomycota. Therefore, “MoBA” was developed as an interactive learning medium presenting Basidiomycota material by featuring four Basidiomycota species from different families, thereby providing students with broader insights into the diversity of Basidiomycota species. This study aimed to develop interactive Basidiomycota multimedia and evaluate its feasibility in terms of validity, practicality, and effectiveness. The study employed the ADDIE development model, consisting of five stages: analyze, design, development, implementation, and evaluate. Both qualitative and quantitative data were analyzed. The study involved three experts, namely a material expert, a media expert, and a practitioner, as well as 29 students as respondents. The results showed that the validity scores assessed by the media and material experts were 88% and 98%, respectively. The practicality test yielded a score of 97.6%, while the readability test obtained an average score of 91.2%. The effectiveness test indicated a significance value of 0.000 based on the Wilcoxon test, while the N-Gain score was 0.7822, which was categorized as high. Based on the validity, practicality, and effectiveness results, it can be concluded that the interactive Basidiomycota multimedia meets the feasibility criteria and is suitable for use as a learning medium.