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Analysis of Vitamin C Antioxidant Levels in Lotion Products Circulated in Gisting Market Cosmetic Stores Using the DPPH Method Dinda Pemila Tri Aprilia; Iga Mayola Pisacha; Eva Nursoleha; Nita Windi Lestari
JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) Vol. 5 No. 2 (2026): JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI)
Publisher : LPPM Universitas Dhyana Pura

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Abstract

Abstract Vitamin C is an antioxidant compound widely used in skin care products due to its ability to ward off free radicals and help maintain healthy skin. Lotions on the market often list vitamin C as an active ingredient, but the levels and antioxidant activity can vary from product to product. This study aims to determine the vitamin C content and analyze its antioxidant levels and activity. Vitamin C is an antioxidant compound widely used in skin care products due to its ability to ward off free radicals and help maintain healthy skin. However, its antioxidant levels and activity can vary from product to product. Using a purposive sampling technique, the study samples consisted of ten lotions with different trademarks. For qualitative analysis, KMnO4 reagent was used, while quantitative analysis was carried out by determining vitamin C levels and testing antioxidant activity using the DPPH method with UV-Vis spectrophotometry measurements. The results showed that of the 10 samples tested, 5 samples (L/3, L/5, L/8, L/9, and L/10) showed positive vitamin C levels; the color changed to brown after the addition of KMnO4. The highest vitamin C content was obtained in sample L/3 at 0.0015%, while the lowest content was in sample L/10 at 0.0007%. The results of the antioxidant activity test showed that the IC50 value of sample L/3 was 130.80 µg/mL, L/5 was 97.09 µg/mL, and L/10 was 62.15 µg/mL. Based on the results of the study, it can be concluded that lotion products sold in the Gisting Market cosmetic store have different vitamin C content and antioxidant activity in each sample