<![CDATA[General Background: Hepatitis B virus infection remains a global health problem and liver function tests, including Total Bilirubin, SGOT, and Gamma GT, are used in assessment. Laboratory testing may involve serum or EDTA plasma and can be delayed because of sample delivery, technical problems, or human error. Specific Background: Bilirubin is susceptible to degradation, while SGOT and Gamma GT may vary with specimen handling and delayed processing. Knowledge Gap: The differences in Total Bilirubin, SGOT, and Gamma GT results according to specimen type and specific delay times in suspected Hepatitis B patients required examination. Aims: This study analyzed differences in these three parameters using serum and EDTA plasma at 0, 4, and 6 hours. Results: In an experimental laboratory study conducted at Petrokimia Gresik Hospital in June 2025, 15 serum and 15 EDTA plasma samples were analyzed. Specimen type showed no significant difference in the three parameters (p>0.05). Delay time significantly affected Total Bilirubin (p=0.013), whereas SGOT (p=0.104) and Gamma GT (p=0.192) showed no statistically significant differences. Total Bilirubin decreased with delay in both specimen types. Novelty: The study presents a comparative assessment of three liver function parameters across serum and EDTA plasma with 0-, 4-, and 6-hour delays in suspected Hepatitis B patients. Implications: The findings indicate that delay time should receive attention in Total Bilirubin testing, while SGOT and Gamma GT showed no statistically significant differences under the tested conditions. Key Findings Highlights Specimen type produced no statistically significant variation across the three parameters. Bilirubin values declined progressively from baseline through six-hour observation. The two enzyme measurements remained statistically nonsignificant under the tested conditions. Keywords : Hepatitis B, Specimen Variation, Total Bilirubin, EDTA Plasma, Liver Function Tests]]>
