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All Journal Medical Laboratory Technology Journal THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST JPM (Jurnal Pemberdayaan Masyarakat) Journal of Epidemiology and Public Health Medical Technology and Public Health Journal Medicra (Journal of Medical Laboratory Science/Technology) Academia Open Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat Jurnal Abdimas Kartika Wijayakusuma Procedia of Social Sciences and Humanities Prosiding University Research Colloquium

Puspitasari Puspitasari

Universitas Muhammadiyah Sidoarjo

Author-ID : 2505379

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Effects of Education, Nutrition Status, Treatment Compliance, Family Income, and Family Support, on the Cure of Tuberculosis in Mojokerto, East Java -, Puspitasari; Mudigdo, Ambar; Adriani, Rita Benya
Journal of Epidemiology and Public Health Vol 2, No 2 (2017)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.101 KB)

Background: Tuberculosis is an important global public health issue. Countries around the world have committed to control the disease with various programs. However, the cure of Tuberculosis treatment in many countries is still low, which can hamper the success of Tuberculosis control program. Productivity of Tuberculosis patients continues to decrease that leads to socioeconomic burden. This study aimed to examine the effects of education, nutrition status, treatment compliance, family income, and family support, on the cure of Tuberculosis.Subjects and Method: This was an observational analytic study with case control design. The study was conducted in Mojokerto, East Java, from April to May, 2017. A total sample of 108 Tuberculosis patients were selected for this study by fixed disease sampling. The sample consisted of 35 uncured cases of Tuberculosis and 73 cured cases of Tuberculosis. The dependent variable was cure of Tuberculosis. The independent variables were education, nutrition status, treatment compliance, family income, and family support. The data was collected by a set of questionnaire and analyzed using path analysis.Results: Nutritional status (b= 1.31; 95% CI = 0.41 to 2.22; p=0.004) and treatment compliance (b= 1.07; 95% CI= 0.17 to 1.97; p=0.019) directly and positively affect the cure of Tuberculosis. Nutritional status was affected by high education (b=1.62; 95% CI =0.62 to 2.63; p=0.002), family income (b=1.66; 95% CI =0.70 to 2.62; p=0.001), and strong family support (b=1.50; 95% CI =0.36 to 2.63; p=0.010). Treatment compliance was affected by high education (b= 0.84; 95% CI = -0.14 to 1.81; p=0.093), family income (b= 1.36; 95% CI =0.42 to 2.30; p=0.005), and strong family support (b=2.08; 95% CI =0.96 to 3.19; p<0.001).Conclusion: Cure of Tuberculosis is directly affected by nutritional status and treatment compliance. Education, family support, and family income, indirectly affect cure of Tuberculosis.Keywords: cure of Tuberculosis, education, nutrition status, family income, family support, treatment complianceCorrespondence:Ã‚Â Puspitasari. Masters Program in Public Health, Sebelas Maret University, Jl. Ir. Sutami 36 A, Surakarta 57126, Central Java. Email: puspita90@yahoo.co.id. Mobile: +6281333060714.Journal of Epidemiology and Public Health (2017), 2(2): 141-153https://doi.org/10.26911/jepublichealth.2017.02.02.05

Detection of Salmonella typhi Using Multiplex and Monoplex PCR in Tifoid Fever Patients Andika Aliviameita; Meryn Linanda Agustin; Anggie Wedha Puspita; Miftahul Mushlih; Puspitasari Puspitasari; Yanik Purwanti; Arief Wisaksono
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Typhoid fever is a disease caused by Salmonella typhi infection. This disease is still a global problem. The purpose of this study was to develop a rapid detection method for Salmonella using molecular methods utilizing Inv A and Fim-C Genes. Two methods compared, which are Multiplex and monoplex PCR. The sample is in the form of the patient's blood, which is stored at 3 ml EDTA vacutainer tube consisting of 10 positive samples and five negative samples. The criteria for samples used previously have tested by widal/ immunoserology (titer more than 1/160). The results showed that multiplex PCR for detection of Salmonella using the Inv A gene and fim c gene not recommended because too many bands produced. The application of monoplex in the Inv A gene gives better results than the Fim C gene. In conclusion, the monoplex application on Inv A gene recommended than Fim C gene used to detect S. typhi in human blood samples.

The Effects of Hyperbaric Oxygen Therapy on Blood Glucose Levels and Lipid Profile in Diabetes Mellitus Patients Andika Aliviameita; Nevy Elensya; Puspitasari Puspitasari; Sri Mukhodim Faridah Hanum
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Diabetes mellitus and its complications are still a major health problem in the world. Diabetes mellitus is closely related to the lipid profile, especially concerning the cardiovascular disease. A method that can be used to treat diabetes mellitus is hyperbaric oxygen therapy (HBOT). This study aims to observe changes in blood glucose levels and lipid profiles in patients with diabetes mellitus with hyperbaric oxygen therapy. The determination of blood glucose levels and lipid profiles is done by photometry. A total of 30 patients with diabetes mellitus examined for their blood glucose level and lipid profile before and after hyperbaric oxygen therapy. In the Paired T-test, there were significant decrease in blood glucose levels (p= 0,000), total cholesterol (p= 0,000), and triglycerides (p= 0,000) after hyperbaric oxygen therapy. The conclusion of this study shows there is a significant decrease in blood glucose level and lipid profile (total cholesterol, and triglycerides) after hyperbaric oxygen therapy.

Quality of Genom in Type II Diabetes Mellitus Patients in Viewed of Temperature, Storage Duration, Number of Leukocyte Puspitasari Puspitasari; Evi Rinata; Rohman Dijaya; Siska Aprilia; Dina Trikumalasari; Livia Nur Azzah; Qilmia Fanani; Miftahul Mushlih; Andika Aliviameita; Dian Delta
Medical Laboratory Technology Journal Vol. 5 No. 2 (2019): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Type II Diabetes Mellitus is a disorder caused by genetic and environmental factors. Molecular analysis of T2DM abnormalities has been carried out. But the analysis in sample preparation especially the stages of DNA isolation has not been done much. The aims of this study are to investigate the effect of temperature, storage duration and level of white blood cell (WBC) with Genome Quality in T2DM. The treatment (n:10) which were divided into several tubes and then stored at 4 °Ϲ, 25 °Ϲ and 32 °Ϲ for 21 days. To determining storage duration effect, we use periodically isolation of DNA (3, 15 and 30 days) after sampling. The effect of WBC with DNA quality was carried out using 17 samples. DNA isolation was done by the DNA Isolation Kit manual without modification and then tested qualitatively and quantitatively. Based on this research, it can be concluded there is a correlation between the numbers of WBC with DNA quality. The higher the number of WBC, the higher DNA concentration (r: 0.818. p value: 0.000). The concentration of DNA at a temperature of 4°Ϲ (135.1 ± 165.2 ng / µl) was higher compared with the temperature treatment 25 ° Ϲ (29.7 ± 36.5 ng / µl) and 32 ° Ϲ (22.14 ± 7.13 ng / µl) (p

Korelasi Profil Darah Dengan CRP Serum pada Pasien Diabetes Mellitus dengan Ulkus Diabetikum Andika Aliviameita; Puspitasari Puspitasari; Yanik Purwanti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.7242

Diabetes mellitus is a metabolic disorder that experiences hyperglycemia and causes various complications, including diabetic foot ulcers (DFU). Hyperglycemia can cause chronic inflammation and decreased function of immune cells. This study aims to determine the correlation between blood profiles and serum C-Reactive Protein (CRP) in diabetes mellitus patients with diabetic ulcers. A total of 30 blood samples of diabetes mellitus patients with blood tests and serum CRP. Blood profile examination used an automatic method with a hematology analyzer, erythrocyte sedimentation rate (ESR) using the Westergreen method, and serum CRP using a semi-quantitative method. In the correlation regression multiple test knowed that the parameters associated with CRP serum are hemoglobin (r = 23.553; p = 0.015), hematocrit (r = -8.678; p = 0.016), leukocyte count (r = 2.098; p = 0.004), and platelet count (r = -0.112; p = 0.002) because it has significance (p < 0.05). Meanwhile, the parameters of erythrocyte count (r = -8.717; p = 0.359), and ESR (r = -0.142; p = 0.109) had no effect on serum CRP levels because they had significance (p> 0.05). The conclusion of this study shows there is a strong correlation (r = 0.777) between blood profile and serum CRP in diabetes mellitus patients with diabetic foot ulcers.

KORELASI ANTARA PROFIL HEMATOLOGI DENGAN PROCALCITONIN PADA PASIEN TERKONFIRMASI COVID-19 puspitasari Puspitasari; Andika Aliviameita; Evi Rinata; Dira Nadila
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 1 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i1.7290

Covid-19 is an infectious disease caused by SARS-CoV 2 and has spread very quickly. Several laboratory examinations were carried out on Covid-19 patients including molecular examinations, hematological profiles, and serology. This study aims to determine the relationship between leucocyte, d-dimer, hemoglobin and thrombocyte against procalcitonin in patients with Covid-19. This was an observational analytic study with cross sectional design. A sample of 50 was taken selectively at Aisyiyah Siti Fatimah Tulangan Hospital in November 2020 - January 2021. Multiple linear regression test results showed relationship between leucocyte with procalcitonin (b = -0.64; p = 0.426), d-dimer with procalcitonin (b = 2.702; p = 0.010), hemoglobin with procalcitonin (b = 0.656; p = 0.255), and thrombocyte with procalcitonin (b = 0.037; p = 0.002) . Based on the results, it is found that there is relationship between leucocyte, d-dimer, hemoglobin, and thrombocyte againts procalcitonin in Covid-19 patients.

The Effect Of Lemon (Citrus limon) Juice on Serum BUN And Creatiinin Levels In Hyperuricemia Rattus norvegicus Siti Nofiani Mufida; Puspitasari Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol 3 No 1 (2020): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v3i1.655

Hyperuricemia is a condition of increasing levels of uric acid in blood serum above normal values. Citrus limon contains flavonoids which have the ability to regenerate kidney function and play a role in reducing oxidative stress. The purpose of this study is to determine the effect of lemon juice (Citrus limon) on serum BUN levels and creatinine in Rattus norvegicus rats that experienced hyperuricemia. The research design used is quantitative analysis using experimental methods. This study used 24 Rattus norvegicus rats were divided into 6 groups. group 1 as normal control, group 2 as hyperuricemia control, group 3 with chicken liver juice and allopurinol 10 mg / g BB, group 4 with chicken liver juice and lemon juice 0,9 ml / 200g BB, group 5 with giving chicken liver juice and lemon juice a dose of 1.8 ml / 200 g BB, group 6 with the administration of chicken liver juice and lemon juice with a dose of 3.6 ml / 200 g BB orally. White mice are taken blood for BUN (Blood Urea Nitrogen) and creatinine. Based on the results of the One Way ANOVA statistical test, there was an effect of Citrus limon juice on BUN levels in group 5 with a value of 18,425 mg/dl, while at creatinine levels which affected Citrus limon juice in group 5 of 0,250 mg/dl.

Relationship between Blood Glucose, Cholesterol and Blood Pressure in Diabetes Mellitus Patients with Diabetic Ulcers Siti Khosnaini; Puspitasari
Academia Open Vol 4 (2021): June
Publisher : Universitas Muhammadiyah Sidoarjo

Diabetes mellitus is a chronic disease caused by heredity or environment. According to Riskesdas, 2018 states that based on a doctor's diagnosis, the number of diabetes mellitus cases in Indonesia has increased to 2% compared to the results of Rikesdas in 2013 which was only 1.5%. The purpose of this study was to determine the relationship between blood glucose, cholesterol and blood pressure in diabetes mellitus patients with diabetic ulcers. The research design used was cross sectional. The research sample was 30 patients with diabetes mellitus with diabetic ulcers at Wonoayu Luka House. This research was conducted from February to March 2021. The results showed that there was no relationship between blood glucose and cholesterol with blood pressure (r=0.043, r=-0.199) after the Pearson correlation test was performed.

Peningkatan Derajat Kesehatan Masyarakat Melalui Pemeriksaan Kesehatan dan Edukasi Pola Hidup Sehat Puspitasari Puspitasari; Evi Rinata; Agus Salim
Jurnal Pemberdayaan Masyarakat Vol 4 No 1 (2019): Mei
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM)

Improving the level of public health can be started by increasing sufficient knowledge about healthy lifestyles. The period of the elderly is a period of life that needs attention because this period is susceptible to disease. Community service activities aim to broaden the views on the importance of health for the elderly. This activity was carried out at the elderly Posyandu, Jiken Village, Tulangan, Sidoarjo in March to April 2019. The method used was observation, Point of Care Testing (POCT) training for Posyandu cadres, counseling and consultation regarding healthy lifestyles. The result of this activity is that Posyandu cadres can do Point of Care Testing (POCT) and can implement it continuously because the simple medical devices they have are complete. In addition, the elderly people are increasingly enthusiastic to come to the posyandu. This can be seen from the list of visits and activeness of the elderly in participating in the whole series of activities while in the posyandu.

Stabilitas Sampel Darah Terhadap Profil Hematologi Dengan Metode Otomatis puspitasari Puspitasari; Andika Aliviameita
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.12667

Sample stability, part of the pre-analytic phase is an important component that can affect clinical laboratory results. Handling of blood samples and storage methods can significantly affect the results of hematological examinations. This study aims to determine the effect of delay time and storage temperature on the results of the examination of leukocyte count, hemoglobin level, erythrocyte count, hematocrit level, and platelet count. The type of research used is a quantitative analysis using laboratory experimental methods. The research was carried out in the hematology laboratory of the Medical Laboratory Technology Study Program, University Muhammadiyah of Sidoarjo in September - December 2021. The results of the Two Way ANOVA test showed that there was no statistically significant effect between the length of time delay and storage temperature on the results of the examination of leukocyte count, hemoglobin level, number of erythrocytes. Friedman test results showed that there was a statistically significant effect between the length of time of delay and storage temperature on hematocrit levels and platelet counts.

Co-Authors Agus Salim Ahmad Shobrun Jamil Aliviameyta , Andika Ambar Mudigdo Andika Aliviameita Andika Aliviameyta Anggie Wedha Puspita ARDIANSYAH, SYAHRUL Arief Wisaksono Chylen Setiyo Rini Dian Delta Dina Trikumalasari Dira Nadila Evi Rinata Faridah Hanum, Sri Mukhodim Filberta, Yusnita Firmansyah, Nukayo Huesein, Wa Ode Asma`Ul Husna Jamilatur Rohmah Livia Nur Azzah Maulidiyah , Hikmatul Meryn Linanda Agustin Miftahul Mushlih Murosidah, Pratasya Liyaajul Mushlih , Miftahul Nevy Elensya Puspita, Anggun Qilmia Fanani Ramadhan, Syahrul Ibnu Mubaroq Rita Adriani Benya Adriani Rohman Dijaya Silvia Ariyanti Siska Aprilia Siti Khosnaini Siti Nofiani Mufida Siti Zaenab Salim Sofi Rahayu, Bella Sri Mukhodim Faridah Hanum Syabandia, Sitti Nur Qablyatin Tjondro, Lidia Agustin Widowati, Hesty Yanik Purwanti

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