Out-of-Hospital Cardiac Arrest (OHCA) is a life-threatening emergency that requires immediate intervention to improve patient survival. One strategy to increase survival rates is Telephone-Assisted Cardiopulmonary Resuscitation (T-CPR), in which emergency medical dispatchers provide cardiopulmonary resuscitation instructions to bystanders over the telephone. The accuracy of T-CPR implementation is influenced by several factors, particularly the dispatchers' level of knowledge and communication skills. Objective: This study aimed to analyze the relationship between the knowledge level and communication skills of emergency medical dispatchers and the accuracy of T-CPR implementation among Public Safety Center (PSC) dispatchers in South Tangerang City. Methods: This quantitative study employed a cross-sectional design. The study involved 50 PSC dispatchers selected using a total sampling technique. Data were collected using knowledge and communication skills questionnaires, along with an observation checklist to assess T-CPR accuracy. Data were analyzed using univariate analysis and the Chi-Square test with a significance level of α = 0.05. Results: Most respondents demonstrated good knowledge (70.0%), good communication skills (64.0%), and good T-CPR accuracy (60.0%). The Chi-Square analysis revealed a significant relationship between knowledge and T-CPR accuracy (p = 0.009), as well as between communication skills and T-CPR accuracy (p = 0.004). Conclusion: There were significant relationships between the knowledge level and communication skills of emergency medical dispatchers and the accuracy of T-CPR implementation among PSC dispatchers in South Tangerang City. Continuous education and training are recommended to enhance dispatcher competence and improve the quality of T-CPR implementation.