<![CDATA[This study aimed to evaluate the physiological effects of butterfly pea (Clitoria ternatea L.) infusion on hematological parameters, fagosistic activity, and gastrointestinal morphology of male mice (Mus musculus). Butterfly pea is known for its high antioxidant content, particularly flavonoids and anthocyanins, which possess diverse biological activities, including anti-inflammatory and immunomodulatory properties. A post-test only control group and treatment group design was used with 10 male mice aged 2–3 months, divided into a control group administrated plain drinking water and a treatment group receiving a daily infusion of dried butterfly pea flowers for 10–12 days. Blood samples were collected at the end of treatment to assess erythrocyte count, leukocyte count, hemoglobin concentration, and phagocytic activity. Gastrointestinal organs were observed macroscopically to evaluate potential toxic effects. The results showed that butterfly pea infusion significantly increased erythrocyte count by 190.9% compared to the control group (p = 0.0005). Hemoglobin levels also increased by 61.3%, although not statistically significant (p = 0.0666). These improvements suggest erythropoiesis and protection of red blood cells from oxidative damage due to the plant’s antioxidant components. In contrast, leukocyte levels decreased by 33.5% (p = 0.270), indicating a possible anti-inflammatory modulation rather than reduced immune capacity. Phagocytosis analysis revealed a tendency toward increased macrophage activity in the treatment group, demonstrating that the infusion may enhance innate immune function. The leukocyte count tended to decrease insignificantly, indicating anti-inflammatory effects and immunomodulatory activity without compromising the body’s defensive function. Morphometric analysis of the digestive tract showed no abnormalities or toxic effects, with all organs maintaining normal color, structure, and texture. Macrophage phagocytosis activity increased significantly, indicating a strengthening of the innate immune response and confirming the safety of butterfly pea flower tea as a herbal remedy.]]>
