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All Journal Journal Health Applied Science And Technology (JHAST)
Trisna Citra
Poltekkes Kemenkes Banten
Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Public Health

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The Effectiveness of Neutral Buffered Formalin in Preserving Urine Erithrocytes with Delayed Testing Mardyana Nizar; Trisna Citra
jurnal kesehatan terapan sains dan teknologi Vol 4 No 2 (2026): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v4i2.120

Abstract

Urine erythrocyte examination is one of the important parameters in urinalysis to detect hematuria and abnormalities of the urinary system. Delays in examination often become a challenge in laboratories, as erythrocytes are prone to lysis or morphological changes that may affect result accuracy. This study aimed to determine the effectiveness of Neutral Buffered Formalin (NBF) as a fixative in maintaining the count and morphology of urine erythrocytes at different examination delay times: 0 hours as control, and delays of 3 hours and 6 hours. Urine samples were collected using purposive sampling, and erythrocyte sediment examination was performed using a light microscope. Data analysis employed the Dependent Sample T-Test to compare results across each delay interval. The findings showed that the use of NBF was able to maintain the stability of urine erythrocytes up to a 6-hour delay, with differences in count and morphology that were not statistically significant (p > 0.05). These results indicate that NBF is effective as a fixative to preserve the quality of urine erythrocyte examination despite delays in analysis.
Co-Authors Mardyana Nizar