This study aimed to evaluate the effects of ZnSO₄ application on biomass accumulation, grainyield, and Zn content in three rice varieties. The research was conducted in paddy fields locatedin Maja Village, Margapunduh Subdistrict, Pesawaran District, Lampung. The treatments werearranged in a factorial design using a strip plot with three replications. The treatments werearranged by a factorial design (4×3) using a strip plot in a completely randomized block design(CRBD) with three replications. The first factors were four application methods of ZnSO₄,consisting of: control (P0), seed priming with 0.25% ZnSO₄ (P1), seed priming with 0.25% ZnSO₄and foliar spray with 0.5% ZnSO₄ at the vegetative stage (P2), seed priming with 0.25% ZnSO₄and foliar spray with 0.5% ZnSO₄ at the generative stage (P3), and seed priming with 0.25%ZnSO₄ and foliar spray with 0.5% ZnSO₄ at both vegetative and generative stages (P4). Thesecond factors were three rice varieties, consisting of Ciherang (V1), Inpari-32 (V2), and InpariIR Nutrizinc (V3). The observed variables included fresh and dry biomass at vegetative andharvest stages, weight of 100 grains, harvest index, and milled dry grain. The results showed thatthe combination of ZnSO₄ seed priming and foliar spray at both vegetative and generative stagessignificantly increased biomass accumulation, grain yield, and Zn content. Ciherang and Inpari-32 varieties produced higher yields compared to Inpari IR Nutrizinc. This study indicates thatagronomic biofortification through ZnSO₄ application is effective in improving rice yield andnutritional quality, particularly Zn content, which plays a vital role in addressing stunting.