<![CDATA[Siti Latipah, Miftahul Jannah & Muhammad Rifqi Hariri 2026. DNA Barcoding and Taxonomic Placement of Alocasia flemingiana Yuzammi & A. Hay based on trnL-trnF intergenic spacer region. Floribunda 9(1) 27—33 — Alocasia flemingiana Yuzammi & A. Hay is a species of the family Araceae for which molecular data remain limited. The high morphological similarity among Alocasia species often complicates species identification and taxonomic classification. This research tended to characterize the trnL-trnF intergenic spacer (IGS) sequence of A. flemingiana and examine its phylogenetic position employing DNA barcoding. A 450 bp trnL-trnF IGS sequence was successfully amplified and examined. BLAST analysis revealed high sequence similarity with several Alocasia species, with query coverage ranging from 98% to 100%, E-values of 0.0, and percent identities of 99.12% to 100%. The highest similarity was observed with Alocasia sp. PSA280 (100% identity, 98% query coverage), while several accessions of A. longiloba also showed high sequence similarity (>99% identity). Phylogenetic reconstruction using the Maximum Likelihood method under the Tamura 3-parameter model with 1,000 bootstrap replicates placed A. flemingiana in a well-supported clade with A. longiloba (ON777914.1), supported by a bootstrap value of 92%. These results demonstrate that the trnL-trnF IGS region is useful for species identification and phylogenetic inference in Alocasia, while providing molecular evidence supporting the taxonomic placement of A. flemingiana. This study contributes to the molecular characterization of A. flemingiana and highlights the value of DNA barcoding in plant taxonomy and biodiversity research. ]]>
