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Muhammad Rifqi Hariri
Pusat Riset Biosistematika dan Evolusi, -BRIN

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Updated Diversity of Costaceae in Java with the First Record of Monocostus K.Schum. Muhammad Hisyam Baidlowi; Arifin Surya Dwipa Irsyam; Muhammad Rifqi Hariri
Floribunda Vol. 9 No. 1 (2026): Floribunda
Publisher : Penerbit BRIN (BRIN Publishing)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/floribunda.2026.15431

Abstract

Muhammad Hisyam Baidlowi, Arifin Surya Dwipa Irsyam, Muhammad Rifqi Hariri 2026. Updated diversity of Costaceae in Java with the first record of Monocostus K.Schum. Floribunda 9(1) 9—13 — Costaceae Nakai comprises eight genera distributed throughout tropical regions, but in Java the family was previously represented by only two genera, Costus and Hellenia. Recent studies have updated the diversity of Costaceae in Java, particularly within Costus, by documenting newly cultivated, naturalized, and escaped introduced species based on field surveys, herbarium studies, and morphological analyses, increasing the number of recorded species to 15. Field investigations conducted between 2023 and 2025 further revealed the occurrence of an additional genus, Monocostus, in Java, currently known only from cultivated specimens. This finding expands the generic representation of Costaceae on the island and provides an important baseline for monitoring introduced ornamental taxa, assessing their potential for naturalization, and supporting  future floristic, taxonomic, and conservation studies.  
DNA Barcoding and Taxonomic Placement of Alocasia flemingiana Yuzammi & A. Hay Based on trnL-trnF Intergenic Spacer Region Siti Latipah; Miftahul Jannah; Muhammad Rifqi Hariri
Floribunda Vol. 9 No. 1 (2026): Floribunda
Publisher : Penerbit BRIN (BRIN Publishing)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/floribunda.2026.15889

Abstract

Siti Latipah, Miftahul Jannah & Muhammad Rifqi Hariri 2026. DNA Barcoding and Taxonomic Placement of Alocasia flemingiana Yuzammi & A. Hay based on trnL-trnF intergenic spacer region. Floribunda 9(1) 27—33 — Alocasia flemingiana Yuzammi & A. Hay is a species of the family Araceae for which molecular data remain limited. The high morphological similarity among Alocasia species often complicates species identification and taxonomic classification. This research tended to characterize the trnL-trnF intergenic spacer (IGS) sequence of A. flemingiana and examine its phylogenetic position employing DNA barcoding. A 450 bp trnL-trnF IGS sequence was successfully amplified and examined. BLAST analysis revealed high sequence similarity with several Alocasia species, with query coverage ranging from 98% to 100%, E-values of 0.0, and percent identities of 99.12% to 100%. The highest similarity was observed with Alocasia sp. PSA280 (100% identity, 98% query coverage), while several accessions of A. longiloba also showed high sequence similarity (>99% identity). Phylogenetic reconstruction using the Maximum Likelihood method under the Tamura 3-parameter model with 1,000 bootstrap replicates placed A. flemingiana in a well-supported clade with A. longiloba (ON777914.1), supported by a bootstrap value of 92%. These results demonstrate that the trnL-trnF IGS region is useful for species identification and phylogenetic inference in Alocasia, while providing molecular evidence supporting the taxonomic placement of A. flemingiana. This study contributes to the molecular characterization of A. flemingiana and highlights the value of DNA barcoding in plant taxonomy and biodiversity research.  
Co-Authors Arifin Surya Dwipa Irsyam Miftahul Jannah Muhammad Hisyam Baidlowi Siti Latipah