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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) JURNAL SAINS PERTANIAN EQUATOR
PUTRI, AYU DIAH PUTU LAKSMI
Plant Breeding And Biotechnology Study Program, Graduate School Of IPB University (Bogor Agricultural University), Jl. Meranti, Kampus IPB Dramaga, Bogor 16680, INDONESIA
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science

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Respon Pertumbuhan Anggrek Phalaenopsis Hibrid pada Media MS dan Berbagai Media Alternatif Secara In Vitro AYU DIAH PUTU LAKSMI PUTRI; ASNAWATI ASNAWATI; NUR ARIFIN
Jurnal Sains Pertanian Equator Vol 7, No 1 (2018): Februari 2018
Publisher : Fakultas Pertanian Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jspe.v7i1.23975

Abstract

Media kultur merupakan salah satu faktor utama yang menentukan keberhasilan perbanyakan dengan kultur in vitro. Media dasar kultur in vitro seperti Murashige and Skoog (MS) dalam pembuatannya rumit serta bahan-bahan kimia yang relatif mahal dan terkadang sulit untuk didapat. Media  alternatif pupuk dapat dijadikan sebagai alternatif karena harga bahan-bahan yang murah, mudah didapat dan kandungan unsur haranya lengkap. Penelitian ini bertujuan untuk mengetahui respon pertumbuhan anggrek Phalaenopsis hibrid pada media MS dan berbagai jenis media alternatif secara in vitro. Penelitian dilaksanakan mulai tanggal 27 Maret hingga 27 Juli 2017 di Laboratorium Bioteknologi Fakultas Pertanian Universitas Tanjungpura. Penelitian ini menggunakan pola Rancangan Acak Lengkap (RAL) satu faktor yaitu media MS, Growquick S, Growmore, Hyponex hijau dan Gandasil D dengan penambahan BAP 1,5 ppm dan air kelapa 100 ml/l. Variabel yang diamati dalam penelitian ini meliputi pertambahan jumlah daun (helai), pertambahan jumlah akar (helai), waktu terbentuk tunas (MST), jumlah tunas (tunas) dan persentase eksplan membentuk tunas (%). Hasil penelitian menunjukkan media alternatif lebih mampu mendorong pembentukan tunas dibandingkan media MS pada persentase eksplan membentuk tunas meskipun respon pertumbuhan anggrek Phalaenopsis hibrid secara umum sama baiknya pada media MS maupun media alternatif. Media alternatif pupuk Gandasil D menunjukkan hasil yang lebih baik pada persentase eksplan membentuk tunas, yaitu sebesar 75% dibandingkan dengan media alternatif lainnya. Kata kunci : Anggrek, In Vitro, Media Alternatif, Media MS, Phalaenopsis Hibrid
Proliferation of porang (Amorphophallus muelleri Blume) from bulbils and leaf cutting treated by NAA and BA Putri, Ayu Diah Putu Laksmi; Wiendi, Ni Made Armini; Santosa, Edi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 3 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v51i3.48958

Abstract

Javanese konjac or iles-iles (Amorphophallus muelleri Blume, Araceae) is a tuber crop native to Indonesia as a source of carbohydrat, also contains a lot of glucomannan, which has high economic value as a raw material in industry. The study aimed to develop the propagation method of A. muelleri from bulbils and leaf cuttings through the plant growth regulator (PGR) application of α-naphthaleneacetic acid (NAA) and benziladenin (BA). The research was conducted from August 2020 to December 2021 at IPB University, Bogor. The study consisted of three experiments based on the type of propagation material. Each experiment used a randomized complete block design with three factors, i.e., NAA concentrations (0, 2, and 4 mg L-1), BA concentrations (0, 5, 10, and 15 mg L-1), and propagation materials (Experiment 1: small, medium, large bulbils; Experiment 2: immature and mature peak leaflet cuttings; Experiment 3: immature and mature base leaflet cuttings). The results showed that there was an interaction of three factors in the bulbils and PGR application, which had a significant effect on the percentage of axillary shoot proliferation and growth. In Experiment 2, PGR application was unable to regenerate peak leaflet cuttings, whereas in Experiment 3 PGR encouraged base leaflet cuttings to form shoots, roots, and bulbils, especially at concentrations of 2 mg L-1 NAA+15 mg L-1 BA and 4 mg L-1 NAA+15 mg L-1 BA. Keywords: α-naphthaleneacetic acid, benziladenin, iles-iles, Javanese konjac, plant growth regulators
Co-Authors Asnawati Asnawati Edi Santosa Ni Made Armini Wiendi Nur Arifin