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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Jurnal Agrotek Tropika Journal of Tropical Crop Science Menara Perkebunan Jurnal Pusat Inovasi Masyarakat

Ni Made Armini Wiendi

Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

Author-ID : 416879

Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance Social Sciences

Published : 30 Documents Claim Missing Document

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Study of Oil Palm (Elaeis guineensis Jacq) In Vitro Embryogenesis using Young Leaves Explants Wiendi, Ni Made Armini; Constantin, Mondjeli; Wachyar, Ade
Journal of Tropical Crop Science Vol 2 No 2 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

This study reported in vitro embryogenesis of oil palm using young leaves as explants. Explants were grown in solid modified MS or Eeuwens medium containing different concentrations of NAA and 2,4-D, i.e. media C1, C2, C3, C4 and C5, M1, M2, M3 and M4, to induce embryogenic calli. Compact and pearly-white, globular calli were obtained from the youngest leaf explants 28 weeks after culture.C1 media (MS medium + 107.41 µM of NAA + 100 mg.L-1 of asparagine + 100 mg.L of glutamine-1) produced the highest percentage of calli formation (30.56%), whereas C4 media (C1 supplemented with 67.86 µM of 2,-D ) was the optimal media for embryogenic callus induction. Direct embryoids were obtained from slightly older leaf explants on the C3 media containing NAA after 36 weeks of culture. However, four subcultures using the same medium with gradual reduction of auxin concentration were not successful to develop embryogenic callus and embryoid cells during the course of this study.

Proliferation of Protocorm-Like Bodies of Dendrobium mannii and Dendrobium mirbelianum in Chitosan-containing Media In Vitro Rahmah, Syifaur; Wiendi, Ni Made Armini; Suwarno, Willy B.; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 2 No 3 (2015): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Dendrobium mannii and Dendrobium mirbelianum were rare and endangered orchid species. In vitro micro propagation could be used to conserve and proliferate these species. The aims of this study were to determine the effects of chitosan on proliferation of the protocorm-like-bodies (PLBs) of in vitro grown Dendrobium mannii and D. mirbelianum. Chitosan has been reported as an important component to promote orchid growth in vitro. In this study chitosan was included in the in-vitro media at the concentrations of 0, 1, 2, 3, and 4 mg.L-1. The highest PLBs formation, clump diameter, and fresh weight on D. mannii were obtained from explants cultured on medium supplemented with 1 mg.L-1 chitosan. After 14 weeks of culture, fresh weight, clump diameter and average number of PLBs of D.mannii were 29.52 %, 33.00 % and 35.88% greater at chitosan (1 mg.L-1) compared to control media. D. mirbelianum treated with low chitosan concentrations (1 and 2 mg.L-1) had 65.71% more leaves than those without chitosan treatment. Chitosan, however, had an adverse effects on growth of both species at 4 mg.L-1. Medium contain 4 mg.L-1 chitosan induced PLBs proliferation of D.mannii, but inhibited PLBs proliferation of D.mirbelianum. These results suggested that chitosan had important roles in PLB proliferation and could be used for the propagation of D. mannii PLBs in vitro. Keywords: rare orchid species, in-vitro

Evaluation of Growth, Flowering and Seed Morphology of Batflower, Tacca chantrieri Andre Ajisyahputra, Nikko Rizky; Palupi, Endah Retno; Krisantini, Krisantini; Wiendi, Ni Made Armini; Mulyono, Joko
Journal of Tropical Crop Science Vol 4 No 2 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Tacca chantrieri is a perennial plant belongs to Taccaceae family that has the potential to be marketed as an ornamental plant due to its unique shape and dark color inflorescence. To date, very limited publication on T. chantrieri are available. The aim of this research was to examine plant growth pattern and flower phenology of T. chantrieri from West Borneo, Indonesia, and from Queensland, Australia in relation to their potential development as ornamental pot plants. The study was extended to examine T. chantrieri seed morphology with an inclusion of an accession from Thailand. The field experiment was conducted at the Leuwikopo experimental station in Darmaga, Bogor, Indonesia. Seed morphology examination was conducted at the Histology Laboratory of Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. T. chantrieri from West Borneo blooms six times a year whereas the Queensland accession twice a year. West Borneo accession had smaller bractea, fewer numbers of flowers per inflorescence and shorter flower stalk than Queensland accession. In addition, T. chantrieri Queensland grew faster than Borneo with the one leaf formed every two to three weeks, whereas it was at least four weeks in the Borneo accession. Both accessions were susceptible to leaf brown spot disease caused by Cercospora taccae. T. chantrieri Queensland seeds are oval dark brown, whereas West Borneo and Thailand accessions were shaped like kidneys (reniform) with a lighter brown color. This study demonstrated that there is great diversity in T. chantrieri including plant morphology, seed shape and color, which are important for species identification and for development of T. chantrieri as ornamental pot plant.

In Vitro Adventitious Shoot Proliferation of Three Basil Species (Ocimum sp. L.) by Addition of Naphthalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP) Wiendi, Ni Made Armini; Putri, Devina Daratyama
Journal of Tropical Crop Science Vol 4 No 3 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Basils are mainly used as food flavoring and source of aromatic oil, but the leaves have also been used for herbals and cosmetics. Basil is propagated by seeds but the germination is often low in the field. This study aims to investigate the effect of different concentrations of NAA (Naphthalene Acetic Acid) and BAP (Benzyl Amino Purine) to induce the adventitious shoot proliferation of three species of basil (Ocimumsp. L.) in vitro. The research was conducted at the Tissue Culture Laboratory II of the Department of Agronomy and Horticulture, Bogor Agricultural University (IPB) from November 2016 till October 2017. This research consisted of three separate experiments with one basil species for each experiment, Lemon Basil, “Greek Minette”, and “Thai Siam Queen”. The experiments were organized in a completely randomized block design with two factors: NAA concentrations (0, 0.5, and 1 mg.L-1) and BAP (0, 1, 2, and 3 mg.L-1) using Murashige and Skoog (MS) medium supplied with 25 ml.L-1 coconut water. The three basil species responded differently to NAA and BAP in the media. The optimum concentration of NAA and BAP to induce callus growth in Lemon Basil was 0.5 mg.L-1 and 3 mg.L-1, respectively, with BAP alone showing the best effect on proliferation. In “Greek Minette”, however, the addition of NAA and BAP had no significant effect on the callus growth, proliferation, height, and number of leaf, node and root per explant. Media without growth regulators provide the best results in increasing height, explant proliferation rate, number of nodes and shoots, and percentage of proliferating shoots of “Thai Siam Queen”.Keywords: cytokinin, “Greek Minette”, Lemon Basil, “Thai Siam Queen”, synthetic auxin

In Vitro Polyploidy Induction of Patchouli (Pogostemon Cablin Benth.) by Colchicine Afifah, Ulil Azmi Nurlaili; Wiendi, Ni Made Armini; Maharijaya, Awang
Journal of Tropical Crop Science Vol 7 No 01 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Patchouli (Pogostemon cablin Benth.; 2n = 32) is an aromatic herbaceous plant commonly cultivated for use in the the fragrance industry. As patchouli is propagated by cuttings polyploidization induction by colchicine treatment was conducted to obtain a new genotype with high patchouli alcohol content. This research aimed to increase patchouli genetic diversity with colchicine treatment by the formation of a polyploid plant. Axillary buds from single node cutting of aseptic plantlets were used as the explants. The experiment was arranged using a factorial completely randomized design with two factors, namely the concentration of colchicine and the immersion duration. Genetic diversity of patchouli was successfully enhanced by adjusting the colchicine concentration and immersion treatment. lethal concentration (LC) of 50% in patchouli was 0.132% and the LC 50% for soaking time was 60.16 hours. The number of chloroplasts, stomatal length and chromosome number increased with increasing ploidy, whereas stomatal density and the number of trichomes decreased. The chromosome number of 16 patchouli mutants in generation 4 was still unstable, and a chimera was found with mixoploidy between 20-80. A hierarchical dendogram clustered 16 patchouli mutants into four different groups.

Optimasi Produksi Bibit Tanaman Kentang (Solanum tuberosum) Kultivar Granola dengan Teknik Fotoautotrofik Sonya Putri Rai; Ni Made Armini Wiendi
Buletin Agrohorti Vol. 3 No. 1 (2015): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Tanaman yang ditumbuhkan dalam kondisi in vitro pada umumnya tidak melakukan fotosintesis, lapisan kutikula dan jaringan pembuluh antara akar dan pucuk tidak berkembang serta stomata belum berfungsi dengan baik sehingga sulit bertahan pada saat aklimatisasi. Teknik fotoautotrofik perlu dikembangkan untuk meningkatkan ketahanan planlet saat dipindahkan ke kondisi ex vitro. Penelitian ini bertujuan mempelajari respon pertumbuhan kentang kultivar Granola yang dikulturkan dengan sistem fotoautotrofik untuk menyediakan bibit kentang yang unggul dan bermutu. Penelitian dilaksanakan di Laboratorium Kultur Jaringan 2, analisis morfologi stomata dilakukan di Laboratorium Mikro Teknik, Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor. Penelitian dilakukan dari bulan November 2014 hingga April 2015. Penelitian ini terdiri dari dua percobaan terpisah. Percobaan pertama menggunakan bahan tanam buku tunggal, percobaan kedua menggunakan bahan tanam pucuk. Rancangan percobaan yang digunakan adalah Rancangan Kelompok Lengkap Teracak (RKLT) dua faktor, yaitu konsentrasi gula dan ventilasi. Pada percobaan pertama diperoleh bahwa peningkatan konsentrasi gula nyata meningkatkan jumlah daun dan buku tunas kentang (Solanum tuberosum). Interaksi gula yang rendah dan penambahan ventilasi menyebabkan peningkatan jumlah stomata dan kloroplas serta mengecilnya diameter stomata daun. Sebanyak 45 % planlet yang ditumbuhkan pada media dengan konsentrasi gula 25 gL-1 dengan ventilasi 1 serta 67 % planlet dari media gula 25 gL-1 dengan ventilasi 2 mampu bertahan selama aklimatisasi dan diduga dapat digunakan untuk produksi bibit. Pada percobaan 2 tidak terdapat planlet yang mampu bertahan pada tahap aklimatisasi.

Pengaruh Pemberian Auksin (NAA) dengan Sitokinin (BAP, Kinetin dan 2ip) terhadap Daya Proliferasi Tanaman Kantong Semar (Nepenthes mirabilis) Secara In Vitro Bagus Setyo Yudhanto; Ni Made Armini Wiendi
Buletin Agrohorti Vol. 3 No. 3 (2015): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Auksin dan sitokinin umumnya digunakan sebagai zat pengatur tumbuh secara in vitro. Sudah ditemukan bahwa kombinasi auksin (NAA) dan sitokinin (BAP, kinetin, dan 2ip) untuk proliferasi tunas Nepenthes mirabilis secara in vitro. Penelitian ini dirancang menggunakan rancangan acak lengkap dua faktor, auksin sebagai faktor pertama dan sitokinin sebagai faktor kedua. Kombinasi auksin dan sitokinin yang digunakan adalah NAA: 1 dan 2 mg / l sedangkan BAP, kinetin, dan 2ip masing-masing sebanyak 0, 2,5, dan 5 mg / l. Kombinasi zat pengatur tumbuh yang ditambahkan ke media Murashige dan Skoog (MS). Hasil terbaik untuk proliferasi tunas yaitu perlakuan tanpa sitokinin dan tambahan NAA 1 mg / l sebanyak 5,2 tunas dalam 10 minggu.

Proliferasi Tunas Adventif Tagetes (Tagetes erecta L.) Kultivar African Crackerjack dengan BAP, GA3, dan IAA Secara In Vitro Muhammad Baidowi; Ni Made Armini Wiendi
Buletin Agrohorti Vol. 5 No. 1 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Tujuan penelitian ini mempelajari proliferasi tunas adventif eksplan hipokotil, epikotil, dan kotiledon Marigold (Tagetes erecta L.) secara in vitro dengan kombinasi BAP, GA3, dan IAA. Percobaan pertama adalah mengenai sterilisasi benih. Eksplan percobaan dua dan tiga adalah epikotil, hipokotil, dan kotiledon dari kecambah aseptik. Eksplan epikotil berhasil terproliferasi sedangkan hipokotil dan kotiledon tidak terproliferasi karena oksidasi fenol. Interaksi BAP dan IAA berpengaruh nyata pada induksi kalus epikotil pada semua minggu pengamatan, sedangkan interaksi BAP dan GA3 tidak berpengaruh nyata pada semua parameter. Komposisi media MS ditambah IAA dan BAP berhasil menginduksi kalus 70.7% dari eksplan epikotoil, 49.3% dari eksplan kotiledon, 53% dari hipokotil. Komposisi media MS ditambah GA3 dan BAP berhasil menginduksi kalus 60% dari eksplan epikotoil, 48% dari eksplan kotiledon, 46% dari hipokotil. Pada percobaan dua, media MS dengan 0.87 mg L-1 IAA + 0.5 mg L-1 BAP dan media MS dengan 0.43 mg L-1IAA + 0.5 mg L-1BAP terbaik dalam menginduksi tunas adventif eksplan epikotil. Pada percobaan tiga, proliferasi tunas adventif tertinggi pada media MS dengan 1.73 mg L-1GA3 + 1.5 mg L-1 BAP. IAA berpengaruh nyata pada eksplan bertunas pada satu 1 MSP, tunas per eksplan pada 3 MSP, dan daun pertunas pada 4 MSP dan 5 MSP, sedangkan BAP berpengaruh nyata pada induksi kalus pada 1 MSP, eksplan bertunas pada 3 MSP, dan tunas per eksplan pada 3 MSP. Pada percobaan tiga, GA3 berpengaruh nyata pada pembentukan akar pada 4 MSP dan 5 MSP, sedangkan BAP berpengaruh nyata pada proliferasi tunas per eksplan pada 3 MSP.

Proliferasi In Vitro Plb Anggrek Dendrobium lasianthera Hasil Induksi Mutasi Genetik dengan Kolkisin Melalui Penambahan Benzyl Adenine Gilar Bawonoadi; Ni Made Armini Wiendi; . Krisantini
Buletin Agrohorti Vol. 5 No. 2 (2017): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Dendrobium lasianthera merupakan spesies anggrek yang endemik di Papua. Proliferasi atau perbanyakan tanaman melalui kultur jaringan merupakan salah satu alternatif dalam memperbanyak spesies tanaman dengan jumlah individu yang terbatas. Keragaman karakteristik pada tanaman hias, baik yang bersifat alami atau hasil dari induksi mutasi, dianggap penting dalam menentukan nilai ekonomisnya. Pengembangan protokol produksi membutuhkan penelitian terkait media yang sesuai untuk memperbanyak tanaman. Penelitian dilakukan dengan mengkulturkan eksplan anggrek Dendrobium lasianthera berupa plb (protocorm-like body) dari anggrek D. lasianthera yang telah diinduksi mutasi melalui perendaman dalam larutan mutagen kolkisin pada penelitian sebelumnya. Pengamatan dilakukan terhadap kemampuan pertumbuhan 960 eksplan dari 16 kombinasi perlakuan (termasuk kontrol) perendaman kolkisin ditumbuhkan pada media MS0 yang ditambahkan sitokinin BA (6-Benzyladenine) sebanyak 1 mgL-1 dan 2 mgL-1. Hasil penelitian menunjukkan bahwa eksplan dengan kombinasi perlakuan perendaman yang berbeda memiliki kemampuan pertumbuhan yang berbeda secara nyata, dilihat dari jumlah daun, akar, plb dan tunas baru yang terbentuk selama periode pengamatan.Perbedaan dalam konsentrasi BA dalam media tidak menunjukkan respon yang berbeda nyata dalam jumlah daun dan akar baru yang terbentuk, namun nyata meningkatkan jumlah tunas baru yang terbentuk dan mempercepat pembentukan tunas. Eksplan yang ditumbuhkan pada media BA 1 mgL-1 memiliki rata-rata waktu awal pembentukan tunas yang lebih singkat dibandingkan dengan media BA 2 mgL-1. Beberapa planlet hasil induksi mutasi menunjukkan perbedaan fenotipe dari planlet kontrol berupa bentuk daun yang berbeda.

Perbanyakan Anggrek Spesies Paphiopedilum glaucophyllum J.J.Smith melalui Proliferasi Tunas Adventif Secara In Vitro Tubagus Kiki Kawakibi Azmi; Ni Made Armini Wiendi
Jurnal Hortikultura Indonesia Vol. 4 No. 3 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTThis research was aimed to determine the effects of BAP and medium on adventitious shoot proliferation of Paphiopedilum glaucophyllum J.J. Smith in vitro. Plantlet of 1 year 9 months old from seed germination in vitro on modified Knudson C medium was used as an explant. This research was arranged in a Factorial Design with three replications. The first factor was different concentration of BAP, consisting of 1 and 2 mg L-1(All combination medium were added with 0.5 mg L-12.4-D). The second factor was different concentration of macro and micro nutrient from MS (Murashige and Skoog) and KC (Knudson C) medium, consisting of 1, ¾, ½, and ¼ concentrationsof macro and micro nutrient. The result showed that plantlets failed to response to the entireadventitious shoot proliferation combination medium within 16 weeks. Concentration of medium affected to leaves and root growth. The optimum growth was achieved at ¾ concentration of macro and micro nutrient from KC medium, combined with 2 mg L-1BAP for leaves growth and 1 mg L-1BAP for root growth. BAP affected callus induction. The highest percentage of callus induction was achieved at 1 mg L-1BAP (48.61%).Key words: BAP, KC, MS, medium, proliferation. ABSTRAKPenelitian dilakukan untuk mempelajari pengaruh zat pengatur tumbuh BAP dan media MS (Murashige & Skoog) dan KC (Knudson C) terhadap kemampuan proliferasi tunas adventif Paphiopedilum glaucophyllum J.J.Smith secara in vitro. Bahan tanaman yang digunakan adalah planlet hasil pengecambahan biji secara in vitro yang telah berumur 1 tahun 9 bulan yang diperoleh dari Pusat Konservasi Tumbuhan, Kebun Raya, Bogor. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan dua faktor perlakuan yang disusun secara faktorial dengan tiga ulangan. Faktor pertama adalah BAP yang terdiri dari 1 dan 2 mg L-1, penggunaan BAP dikombinasikan dengan 0.5 mg L-1 2.4-D. Faktor kedua adalah media (MS dan KC) dengan konsentrasi hara makro dan mikro masing-masing adalah 1, ¾, ½, dan ¼ konsentrasi. Hasil penelitian menunjukkan bahwa proliferasi tunas adventif tidak terjadi pada semua perlakuan. Konsentrasi media (MS dan KC) berpengaruh nyata terhadap pertumbuhan daun dan akar. Pertumbuhan daun dan akar optimum diperoleh pada ¾ konsentrasi hara makro dan mikro pada kedua jenis media. Pertumbuhan daun tertinggi diperoleh pada media KC ¾ konsentrasi hara makro dan mikro ditambah 2 mg L-1. Media KC ¾ konsentrasi hara makro dan mikro ditambah 1 mg L-1menghasilkan jumlah total akar tertinggi. BAP berpengaruh nyata terhadap persentase planlet berkalus. Jumlah planlet berkalus tertinggi diperoleh pada media yang mengandung 1 mg L-1 BAP (48.61%).Kata kunci: BAP, KC, MS, media, proliferasi.

Co-Authors , Krisantini , Nurhasanah , Suharsono Agus Purwito Ajisyahputra, Nikko Rizky Amelia Paramitha Mahanani Awang Maharijaya Bagus Setyo Yudhanto Baidowi, Muhammad Bawonoadi, Gilar Cahyaningsih, Yuni Fitri Constantin, Mondjeli Didy Sopandie Diny Dinarti Edi Santosa Edi Santosa Edi Santosa Endah Retno Palupi Enny Prasetyanti Eny Widajati Fitri Rachmawati Flowrentyka Ferona Haloho G. A. Wattimena Gilar Bawonoadi Hailu, Molla Gebreyohannes Haniefan, Nafarain Agung I Gusti Ngurah Galang Aditya Illahi, Zikril Indah Permata Dewi Irni Furnawanthi Hindaningrum Joko Mulyono Juang Gema Kartika Khalim Rizkiana Bahri Lailatul Qodriyah Agne Verawati Masna Maya SINTA Megayani Sri Rahayu Muhammad Baidowi Musalamah Nurhajati Ansori Mattjik PUTRI, AYU DIAH PUTU LAKSMI Putri, Devina Daratyama Rahmah, Syifaur Rai, Sonya Putri Ratna Trisnawati Sobir Sobir Sonya Putri Rai Sri Rianawati Sudarsono, dan Sugiyanta Syarifah Iis Aisyah Toruan-Mathius, dan Nurita Trikoesoemaningtyas Tubagus Kiki Kawakibi Azmi Ulil Azmi Nurlaili Afifah Wachyar, Ade Willy Bayuardi Suwarno Winarso D. Widodo Winarto, dan Budi Yudhanto, Bagus Setyo

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