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All Journal Jurnal Biologi Tropis
Kuntaman Kuntaman
Universitas Wijaya Kusuma Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

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ESBL-Encoding Gene Patterns in E. coli Isolated from Cat Feces in Surabaya, Indonesia Ema Khoirotun Nisa'; Kuntaman Kuntaman; Masfufatun Masfufatun; Febtarini Rahmawati
Jurnal Biologi Tropis Vol. 26 No. 4 (2026): in Progress
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i4.13422

Abstract

Extended-spectrum beta-lactamase (ESBL)-producing E. coli may be found in cat feces and can act as a reservoir of antimicrobial resistance. This study aimed to determine ESBL-encoding gene patterns in E. coli isolated from cat feces in Surabaya, Indonesia. This descriptive observational laboratory study used 20 ESBL-producing E. coli isolates from cat feces in Surabaya, Indonesia. The blaCTX-M, blaSHV, and blaTEM genes were detected using polymerase chain reaction (PCR), and amplicons were visualized by agarose gel electrophoresis. Data were analyzed descriptively as frequencies and percentages. The blaCTX-M gene was detected in 16 isolates (80%), blaTEM in 6 isolates (30%), while blaSHV was not detected. Gene-pattern analysis showed 13 isolates (65%) carrying blaCTX-M alone, 3 isolates (15%) carrying blaTEM alone, 3 isolates (15%) carrying both blaCTX-M and blaTEM, and 1 isolate (5%) without the three target genes. blaCTX-M was the dominant ESBL gene among E. coli isolates from cat feces. These findings highlight the presence of ESBL-associated genes in E. coli from cat feces and support the inclusion of companion animals in One Health antimicrobial resistance surveillance.
Co-Authors Ema Khoirotun Nisa' Febtarini Rahmawati Masfufatun Masfufatun