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Eva Agustina
Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Ampel Surabaya, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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Effects of Green Tea Kombucha Administration on Sperm Motility and Morphology in Male Mice (Mus musculus) Nova Lusiana; Eva Agustina; Risa Purnamasari
Biota Vol 12 No 2 (2026): Jurnal Biota
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v12i2.31663

Abstract

This study aimed to evaluate the effects of green tea kombucha administration on sperm quality in male mice (Mus musculus), with particular emphasis on sperm motility and morphology. Green tea kombucha is a fermented beverage derived from Camellia sinensis using a symbiotic culture of bacteria and yeast (SCOBY) and contains bioactive compounds with potential antioxidant activity. A completely randomized experimental design was employed, consisting of one control group and four treatment groups that received different oral doses of kombucha for 30 days. Phytochemical analysis confirmed the presence of phenolic compounds, tannins, alkaloids, and terpenoids. The results showed a dose-dependent improvement in sperm motility and the proportion of morphologically normal spermatozoa, with the highest dose producing the greatest effect. Statistical analysis using the Kruskal–Wallis test revealed significant differences among the groups (p < 0.001). These findings suggest that green tea kombucha may improve sperm quality, potentially through antioxidant-related mechanisms, although these mechanisms were not directly investigated in the present study. In conclusion, green tea kombucha shows potential as a functional dietary intervention for supporting male reproductive health.
Co-Authors Nova Lusiana Risa Purnamasari