Biota
Vol 12 No 2 (2026): Jurnal Biota

Effects of Green Tea Kombucha Administration on Sperm Motility and Morphology in Male Mice (Mus musculus)

Nova Lusiana (Department of Nutrition, Faculty of Psychology and Health, Universitas Islam Negeri Sunan Ampel Surabaya, East Java, Indonesia)
Eva Agustina (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Ampel Surabaya, East Java, Indonesia)
Risa Purnamasari (Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Sunan Ampel Surabaya, East Java, Indonesia)



Article Info

Publish Date
25 Aug 2026

Abstract

This study aimed to evaluate the effects of green tea kombucha administration on sperm quality in male mice (Mus musculus), with particular emphasis on sperm motility and morphology. Green tea kombucha is a fermented beverage derived from Camellia sinensis using a symbiotic culture of bacteria and yeast (SCOBY) and contains bioactive compounds with potential antioxidant activity. A completely randomized experimental design was employed, consisting of one control group and four treatment groups that received different oral doses of kombucha for 30 days. Phytochemical analysis confirmed the presence of phenolic compounds, tannins, alkaloids, and terpenoids. The results showed a dose-dependent improvement in sperm motility and the proportion of morphologically normal spermatozoa, with the highest dose producing the greatest effect. Statistical analysis using the Kruskal–Wallis test revealed significant differences among the groups (p < 0.001). These findings suggest that green tea kombucha may improve sperm quality, potentially through antioxidant-related mechanisms, although these mechanisms were not directly investigated in the present study. In conclusion, green tea kombucha shows potential as a functional dietary intervention for supporting male reproductive health.

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Journal Info

Abbrev

biota

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

Description

Jurnal biota is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biology, with particular attention paid to the exploration and biodiversity Wetland tropical—and especially Indonesian—biodiversity. Original research ...