<![CDATA[The anticoagulant component works by binding to metal ions and removing calcium ions, thereby preventing thrombin formation and the formation of clots. However, excessive use of anticoagulants (EDTA) combined with improper waste management can result in waste ending up in water bodies, where it binds to toxic heavy metal ions, causing the metals to dissolve and be carried by water flow—which in turn triggers contamination and exposure to aquatic ecosystems. Parijoto (Medinilla speciosa) is an endemic plant of Kudus that grows in the Mount Muria region. According to Ismah et al., 2025, this plant contains bioactive compounds such as flavonoids, tannins, and glycosides. Flavonoids possess properties that inhibit blood clot formation (antithrombotic) and inhibit platelet aggregation (antiplatelet), mechanisms that are part of the anticoagulation process. The objective of this study was to determine and evaluate the effectiveness of Parijoto extract as a natural anticoagulant alternative compared to EDTA in vitro using the Lee-White assay. An experimental design was used with 8 treatment groups, each consisting of 3 human blood samples treated with the same extract volumes: 80 μL, 100 μL, 120 μL, 140 μL, 160 μL, 180 μL, and 200 μL, with 3 replicates per treatment. Data were measured using a 1–6 score scale indicating blood clotting time. The normality test revealed that the data were not normally distributed (p = 0.003); therefore, nonparametric Kruskal-Wallis analysis and a follow-up Mann-Whitney test were performed. The results of the Kruskal-Wallis test showed a significant effect (p = 0.002). The follow-up Mann-Whitney test revealed significant differences between the 80 μL–160 μL extracts and the EDTA control (p < 0.05). For the 180 μL (p = 0.114) and 200 μL (p = 0.317) extracts, no significant differences were found compared to the EDTA control. From these results, it can be concluded that Parijoto extract has the potential to be effective as a natural anticoagulant with an optimal concentration of 200 μL, which is comparable to the chemical anticoagulant EDTA.]]>
