Purpose – This study aims to analyze the effects of workload and work environment on nurses’ performance, with work stress as a mediating variable among nurses at the IRNA II of Dr. Saiful Anwar Malang Regional General Hospital. The research seeks to examine how workload conditions and workplace factors influence nurse performance through the role of work stress. Design/Methodology/Approach – This study employs a quantitative explanatory research design. Data were collected through a Likert-scale questionnaire distributed to 77 nurses working in the Low Care and High Care rooms of IRNA II, selected using a simple random sampling technique. The data were analyzed using path analysis based on Partial Least Squares (PLS) with SmartPLS 3.0 software. Findings – The findings indicate that workload has a positive and significant effect on nurse performance, while the work environment has a positive but insignificant effect on performance. Furthermore, workload and work environment have negative and significant effects on work stress. Work stress is also found to mediate the relationship between workload and work environment with nurse performance. These results highlight that effective workload management and improvements in the work environment are important to reduce work stress and enhance nurse performance. Originality/Value – This study contributes to healthcare human resource management literature by examining the mediating role of work stress in the relationship between workload, work environment, and nurse performance. The findings provide practical implications for hospital management in developing strategies to optimize workload distribution, improve workplace conditions, and maintain healthcare service quality.