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Effect of Four Antioxidants Combination on Number of Sertoli and Leydig Cells, Sperm Quality and Caspase-3 Expression in Rat Exposed to Cigarette Smoke Conita Yuniarifa; Joko Wahyu Wibowo; Taufiqurrachman Nasihun
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol. 11 No. 2 (2020): December 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v11i2.2465

Introduction: The administration of antioxidant combination has been shown to be more effective than that of individual antioxidant in male infertility.Objective: to determine the effect of different combination of antioxidants (vitamin/vit C, vit E, glutathione and zinc) on the number of Sertoli and Leydig cells, sperm quality and texpression of caspase-3 in rats exposed to cigarette smoke.Methods: This was an experimental study using a post test only control group design. Thirty six rats were randomly divided into 6 groups to receive one of the following combinations of antioxidants: vit C and E, glutathione, zinc (T4-G); vit C and E, glutathione (T3-G); vit C and vit E (T2-G); Glutathione, zinc (T1-G); control positive (CP-G) cigarette smoke exposed only, normal (CN-G). The dose of vit C, vit E, glutathion and zinc was 9 mg/day, 1.8 IU/day, 1.8 mg/day, 0.2 mg/day respectively. All groups were exposed to cigarette smoke (3 cigarettes/day for 21 days), except for the CN-G group. On day 22, sperm samples were taken. Testicular tissue was taken for measurements of sperm quality, sertoli and leydig cell.numbers.Results: There was a significant difference in mean number of Leydig cells, sperm motility, sperm count and caspase-3 between groups (p<0.05). Post hoc LSD showed that the administration of combination of four antioxidant agent increasing the number of Sertoli cells and sperm morphology. The results also showed that group treated with 4 antioxidants combination had the highest number of Leydig cells, sperm motility, sperm counts and the lowest expression of caspase-3.Conclusion:The combination of vitC, vit E, glutathione and zinc affects the number of Sertoli and Leydig cells, sperm quality, expression of caspase-3 in rats exposed to cigarette smoke .

The Effect of Administering Lemon Basil (Ocimum sanctum) Leaves on FSH and LH Estrogen Levels and Size of Ovarian Antral Follicle in Female Balb/C Mice Sri Maryanti; Taufiqurrachman Nasihun; Helfi Amalia
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol. 10 No. 2 (2019): December 2019
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v10i2.2612

INTRODUCTION: Ocimum sanctum have been proven effective in decreasing Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH) levels, and sperm number, and also increase male testosterone levels. However, so far no evidence has been found on the effect of Ocimum sanctum leaves on the levels of estrogen, FSH, LH, and the size of ovarian anthral follicles. OBJECTIVES: To evaluate the effect of Ocimum sanctum leaves on estrogen, FSH, LH and the size of antral follicle of female Balb/C mice.METHODS: In the post test only control group design, 12 female Balb/C mice were assigned into 2 groups. The treatment group (OS-G) was given 0.08 gram of ocimum sanctum leaves, three times a day for 30 days and the control group (C-G) received no ocimum sanctum. Estrogen, FSH anduLH levels wereumeasured on the 30th day, the mice were sacrificed and taken up by their ovaries. HE preparationsuwere then madeuand HE was performed, and the size of the antral follicle of the ovary was measured .RESULTS: Mann Whitney analysisuindicateduthat estrogen and FSH levels in OS-G group were significantly higher compared to that of C-G group, p<0.05. Based on independent t-test, the level of LH in OS-G was significantly higher than that of C-Gugroup, p><0.05. On the contrary, uthe size of ovarianuanthralufollicle in OS-Guwas smaller than that of C-Gugroup, p><0.05. CONCLUSION: Ocimum sanctum increased the levels of estrogen, FSH, LH and decreased the size of ovarian antral follicle in female of Balb/C mice.>

The Influence of Administering Propolis CMCE Extract on FSH, LH, and Testosterone Levels in MSG-Induced Male Wistar Rat Eko Naning Sofyanita; Taufiqurrachman Nasihun; Siti Thomas Zulaikhah
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol. 10 No. 2 (2019): December 2019
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v10i2.4033

Introduction: Monosodium Glutamate (MSG) increase free radicals and effecting on male reproductive hormone balance. CMCE propolis extract can be used as supplement to protect cells from free radical.Objective : To prove the effect of CMCE propolis extract to increase FSH, LH and Testosterone levels in male Wistar rats induced by MSG.Methods : This research was an experimental with post test only control group design used 18 male Wistar rats and divided into 3 groups. Each group on Control (K), P1 and P2 were induced by MSG of 140mg, then on P1 added 8,3mg CMCE propolis extract and P2 10,8mg for 21 days. Then on 22th day of blood was taken through the orbitalis sinus and serum was used for the analysis FSH, LH and Testosteron levels with ELISA method.Results : ANOVA analysis test showed significant differences each group p=0,000(pË‚0,05). Post hoc LSD test showed FSH, LH and Testosterone levels in the 8,3mg dose CMCE propolis extract and 10,8mg were significant different compared with control group p=0,000(pË‚0,05). CMCE propolis extract 10,8mg dose had the significantly highest effect of FSH, LH and Testosterone levels than CMCE propolis extract 8,3 mg.Conclusion : CMCE propolis extract 10,8mg/day for 21 days is proven to increase FSH, LH and testosterone levels in male Wistar rats induced by MSG.

The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate) Rahmawati Rahmawati; Taufiqurrachman Nasihun; Israhnanto Isradji
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol. 10 No. 2 (2019): December 2019
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v10i2.4221

INTRODUCTION: ROS is the most common cause of male infertility. Monosodium glutamate (MSG) is a compound which produces ROS and decreases FSH and LH levels. Continuous Multistage Countercurrent Extraction (CMCE) propolis extract as an antioxidant is evidently capable of reducing ROS concentration.OBJECTIVE: this research aims at evaluating the effect of CMCE propolis extract administration on Leydig cells and Sertoli cells counts and sperm quality of MSG-induced male wistar rats.METHODS: employing the Post Test Only Control Group Design, 18 rats were divided into 3 groups: control group, treated only with MSG (MS-G), and treatment groups, respectively treated with CMCE propolis extract at doses 8.3mg/day (CM8-G) and 10.8mg/day (CM10-G). CMCE propolis extract was given orally for 21 days. At the end of treatment, sperm and testis were taken to evaluate the Leydig cells and Sertoli cells counts and sperm quality. The sperm obtained from epididymis was analyzed using the WHO standards, while Leydig cells and Sertoli cells were counted from preparation using the HE stain method.RESULTS: the Post Hoc analysis result shows that the Leydig and Sertoli cells counts and sperm quality of CM8-G and CM10-G are significantly higher than those of MS-G, p<0.05. The Leydig cells and Sertoli cells counts and sperm quality of CM10-G are significantly higher than those of CM8-G, p><0.05. CONCLUSION: CMCE propolis extract administration evidently increases the Leydig cells and Sertoli cells counts and sperm quality from the perspective of the count, motility and morphology of spermatozoa of MSG-induced male wistar rats.><0.05CONCLUSION: CMCE propolis extract administration evidently increases the Leydig cells and Sertoli cells counts and sperm quality from the perspective of the count, motility and morphology of spermatozoa of MSG-induced male wistar rats.

The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Rats’ Induced with CCl4 Catur Retno Lestari; Titiek Sumarawati; Taufiqurrachman Nasihun
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol. 11 No. 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v11i1.4882

Introduction:Liver exposure to toxic substances will disrupt the metabolic system. Carbon tetrachloride (CCl4) is a hepatotoxic substance causing a liver damage. Continuous multi-stage countercurrent extraction(CMCE) propolis extract contains active balsamic substances, caffeic acid, phenethyl esters and flavonoids.Objective: to prove the effect of CMCE propolis extract on IL-1 levels and the hepatocyte histopathology findingsof male wistar ratsâ€™induced by CCl4.Methods:In the posttest only control group design, the total sample of 28 rats was divided into 4 groups: positive control group (K-0; standard feed); group P-1 (propolis3.6 mg/200 g); P-2 (propolis7.2mg/200gr and P-3 (propolis14.4mg/200g) were respectively given for 14 days,yet those on the 14thday were given CCl4. The IL-1 level was measured with the ELISA method, while the male wistar ratsâ€™hepatocyte histopathology findings was determined by the HE staining methods. IL-1 levelswere analyzed usingOne Way Anova test,followed by the Post Hoc LSD test. Hepatic cell histopathology was analyzed usingKruskal Wallis, followed by Mann-Whitney U.Results:Post Hoc LSD analysis shows that the levels of IL-1 in P-1 (983.54 ±19.67), P-2 (841.58±19.47), and P-3 (755.50±31.63) are significantlylower thanthosein K-0 (1131.69±14.91) withp<0.05. Mann-Whitney U analysis shows that the hepatocyte histopathology in the P-1 group (2.55±0.39), P-2 (1.24±0.94) and P-3 (1.20±0.93) is significantlylower thanthatinK-0 (2.94±0.16) with p<0.05.Conclusion: The administration of CMCE propolis extract may reduce IL-1 levels and improve the male wistar ratsâ€™hepatic cell histopathologyinduced with carbon tetrachloride (CCl4).

The Influence of Continuous Multi Stage Countercurrent Extraction Process (CMCE) Propolis Extract Administration on Superoxide Dismutase Activity and Hepatocytes Fibrosis Degree (An Experimental Study on Male Wistar Rats Induced with Carbon Tetrachloride (CCl4)) Elsayanti Wani; Taufiqurrachman Nasihun; Atina Hussaana
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol. 11 No. 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v11i1.5000

Introduction: Continuous Multi Stage Countercurrent Extraction Process (CMCE) propolis extract containing Caffeic Acid Phenethyl Ester (CAPE) may inhibit the formation of lipid peroxidation. However, it is still unclear whether or not CMCE propolis is able to increase SOD activity and reduce acute hepatocytes damages induced by CCl4. Objective: To evaluate the influence of CMCE propolis extract administration in increasing the SOD activity and reducing the hepatocytes fibrosis of male Wistar rats induced with CCl4.Method: an experimental research with a post-test only control group design. Twenty eight male Wistar rats were divided into four groups. Group C-N was only induced with CCl4, while group CM-3, CM-7, and CM-14 were induced with CCl4 and received CMCE propolis extract respectively with 3.6 mg/200 g BW, 7.2 mg/200 g BW, and 14.4 mg/200 g BW. CMCE propolis was administered for 14 days and then CCl4 was administered on the last day. SOD activity was measured using the colorimetric method, while the hepatocytes fibrosis degree was assessed using Image J Software method with NASH score modification.Results: Post Hoc LSD analysis indicated that SOD activity in group CM-3 (51.67 ± 4.20), CM-7 (70.21 ± 6.26), CM-14 (80.85 ± 4.59) was lower than that in group C-N (23.70 ± 5.12) with P <0.05. Meanwhile, the Mann-Whitney U test showed that the hepatocytes fibrosis degree in group CM-3 (0.85 ± 0.69), CM-7 (1.14 ± 0.37), and CM-14 (0.71 ± 0.48) was significantly lower than that in group C-N (2.28 ± 1.25) with p<0.05.Conclusion: The CMCE propolis extract administration was able to increase the SOD activity and reduce the hepatocytes fibrosis degree of male Wistar rats induced with CCl4

CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4 Chalid Firdaus; Chodidjah Chodidjah; Taufiqurrachman Nasihun
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol. 11 No. 2 (2020): December 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v11i2.5190

Introduction: Liver plays an important role in the metabolic processes possibly exposed to the toxic materials. One of those hepatotoxic substances is carbon tetrachloride. The propolis extract contains the balsamic active substances, CAPE compounds and flavonoids. Flavonoids can prevent from the apoptosis and reduce the inflammation.Objective: To validate propolis extractâ€™s (CMCE method) ability in improving caspase 3 expressions of hepatocytes and IL-6 levels in Wistar male rats induced with carbon tetrachloride.Methods: An experimental study with a post-test only control group design applied to the study. The research subjects were 28 male Wistar rats, divided into four groups. The negative control group (CN-G) was only injected with CCl4, while the experimental groups were administered with the CMCE propolis at the dosages of 3.6 mg/200g (CM3-G), 7.2 mg/200g (CM7-G), and 14.4 mg/200g (CM14-G). The CMCE propolis extract was administered for 14 days and on day 14, the CCl4 was then given. The caspase 3 expression of hepatocytes was measured using HE liver cell preparations, while the IL-6 levels were measured using ELISA method.Results: The results of Mann-Whitney U statistical analysis showed that the hepatocytesâ€™ caspase 3 expressions in CM14-G (2.77 ± 0.531), CM7-G (3.14 ± 0.378), and CM3-G (4.22 ± 0.690) were lower than those in CN-G (5.43 ± 0.535), p<0.05. Meanwhile, the Post Hoc LSD analysis Results showed that IL-6 levels in CM14-G (55.032 ± 9.336), CM7-G (78.362 ± 8.313), and CM3-G (114.975 ± 10.359) were lower those in CN-G (180.301 ± 5.428), p<0.05.Conclusion: the administration of propolis extract (CMCE method) improve the caspase 3 expressions of hepatocytes and Il-6 levels in Wistar male rats induced with carbon tetrachloride.

Propolis Extracted Using CMCE Decreases TNF–α and C-Reactive Protein level of CCl4- Induced Liver Damage in Rats Arief Adi Saputro; Taufiqurrachman Nasihun; Atina Hussaana
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol. 12 No. 1 (2021): June 2021
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v12i1.5467

Introduction: Carbon tetrachloride (CCl4) is a hepatotoxic substance inducing inflammation in liver cells. Inflammation is marked by increase in proinflammatory cytokines level such as tumor necrosis factor alpha (TNF-Î±), and C-reactive protein (CRP) produced by hepatocytes. Propolis extracted using continuous multi-stage countercurrent Extraction (CMCE propolis extract) contains active balsamic, caffeic acid phenetyl ester (CAPE), and flavonoids capable of affecting pro-inflammatory cytokines. Objective: to investigate the effect of CMCE propolis extract on TNF-Î± and CRP levels in CCl4-induced liver damage.Methods: In this study using a post test only control group design, 28 male Wistar rats were divided into four groups. The control group (C-G) was given CCl4 0.4 ml/200 g on day 14. The P3-G, P7-G, P14-G groups were treated with 3.6 mg/200 g, 7.2 mg/200 g, 14.4 mg/200 g CMCE propolis extract for 14 days and given CCl4 0.4 ml/g on day 14. The TNF-Î± and CRP levels were assessed using the ELISA kit one-way ANOVA and Post Hoc test (LSD) were applied for the statistical analysis. Results: TNF-Î± level in P14-G group (3.26 ± 0.93), P7-G (6.17+0.78), and P3-G (10.4±1.34) were significantly lower than that of C-G (17.29+1.56) (p<0.05). CRP levels in P14-G group (0.61±0.09), P7-G (1.38+0.48), and P3-G (12.01±0.65) were significantly lower than those of C-G (2.95+0.668) (p<0.05). The greatest reduction in TNF-Î± & CRP level was found in the group given propolis at a dose of 14.4 mg/200 g.Conclusion: The administration of CMCE propolis extract reduces TNF-Î± & CRP levels in CCl4-induced liver damage.

The Effect of Moderate-Intensity Physical Exercise on Interleukin-6 Level and Lymphocyte Count in Leprosy Reaction Patient Adryan Roy; Taufiqurrachman Nasihun; Titiek Sumarawati
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol. 12 No. 1 (2021): June 2021
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/sainsmed.v12i1.11931

Introduction: Leprosy reaction is an immunity declining reaction marked with inflammation which may cause permanent disability. Physical exercise may improve bodyâ€™s immune system. Objective: To prove the effect of moderate-intensity physical exercise on the interleukin-6 level and lymphocyte count in leprosy reaction patient.Methods: The quasi-experimental research with pretest and posttest and a control group design used 30 respondents consisting of 14 interventions and 16 controls. The intervention group was given moderate-intensity physical exercise 60%-79% in the form of static cycle at a frequency of 4 times weekly, 150 minutes per week. Corticosteroid was always administered to the two groups as a standard therapy. The interleukin-6 level was measured using ELISA method and lymphocyte count was measured using hematology analyzer.Result: The Mann-Whitney analysis shows that the mean difference in IL-6 level between pretest and posttest with the intervention group (-7.1429±25.2369) is significantly lower than that of the control group (8.8125±11.2025), p<0.05. On the contrary, the Independent t-test analysis shows that the mean difference in lymphocyte count between pretest and posttest with the intervention group (0.7407±0.41236) is significantly higher than that of the control group (-0.4500±0.42415), p<0.05. Conclusion: Physical exercise at moderate intensity in the form of aerobics using static cycle at a frequency of 4 times weekly, with duration of 150 minutes per week decreases interleukin-6 level and increases lymphocyte count in leprosy reaction patient.

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